Lca5 (BC052060) Mouse Tagged ORF Clone

CAT#: MG210147

Lca5 (GFP-tagged) - Mouse Leber congenital amaurosis 5 (human) (cDNA clone MGC:62450 IMAGE:5702365)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lca5
• Synonyms: 4930431B11Rik|5730406O13Rik|AV274874|ORF64
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210147 representing BC052060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAAAGAGCCCGAAGTCCAGATATTGAACAAGGAAAAAAAGGAGGCAAACACCCATACTCTCATT
  ATTCGTCTGACCTTGGGTCCTCACCACAGTCCTCTGGCCCCTCATCACCTGTCAACACGACGCCATGTGC
  CAGTACTAGGGAGAAGAATCCCAAAAGACACTTGTCAGACAACCAAGTGCATCACCCAACCGTTAGGAAA
  GTAAGTCCTAAGGCTGTACCCAGCAAAAAGGGCATCCGAGTGGGATTTCGGTCCCAAAGCCTCAACAGAG
  AGCCACTTCGGAAAGATCCAGATATCGTAACAAAGCGGGTTCTTTCTGCCAGACTTCTGAAAATCAACGA
  GCTGCAGAACGAAGTGTCAGAACTACAGGTCAAGTTAGCCCAGCTGCTCAAAGAGAACAAGGCTTTGAAA
  AGCCTTCAGTATCGTCAGGAGAAAGCGCTGAATAAGTTTGAAGATGCGGAGAACGAGATCTCACAACTTA
  TACACCGCCATAACAATGAGATTACTGCGCTCAAAGAACGCTTACGAAAATCTCAGGAGAAGGAGCGGGC
  GACAGAGAAAAGAGTGAAAGAGACAGAAGGTGAACTATTTAGGACGAAATTTTCCTTACAGAAACTGAAA
  AAGATCTCCGAGGCCAGACACCTACCGGAGCGAGACGATTTGGCCAAGAAACTGGTTTCGGCAGAGTTAA
  AATTAGATGACACGGAGAGAAAGATTAAGGAGCTATCGAAGAACCTGGAGCTGAGCACGAACAGTTTCCA
  GCGGCAGCTGCTCGCTGAAAGGAAAAGAGCATTCGAGGCTTACGATGAAAATAAAGTTCTTCAAAAGGAG
  CTGCAGCGGCTCCATCACAAGCTCAAGGAAAAGGAGAAAGAATTGGATATAAAAAACATCTATGCTAATC
  GCCTGCCCAAATCCTCTCCAAAGAAAGAGAAAGAAATCGAAAGGAAACATGTATCCTGCCAGAGTGACTT
  TACAGACCAGTGTACAAAAGGAGTACAGACCGCTGAAGACTTTGAGCTGGAGGACTTTCCTTTCACAGCC
  CAGACAGTTCTCTGCTATGAAAACAGATGGGATGAACCAGAATATCTCTCTTCGTACCTGGAGTACCAGG
  ATCTCAACAAACATGGATCAGAGATGCTAAGTTCAGTGTTGGGACAAGAAGGAAAGTATGATGAAGATGA
  GGACCCATGCTCTGCAAAGCAGGAGGCCAGGAAGCCTGAGAGTGAATGGGCAAGAGAAGAACTTGATAAG
  GTGAAAGGCAAGTCAGCTTTGCTAGGGAGAGCAGAGAAGCTGGCATTGGAAGCTGGGAGATTCCCAACGG
  AAAATTATCAAGCTCAGTCTGTCGATAAATTCGAAGATGAGGCAGAAAGACTAAAGACAGAAATGCTCCT
  TGCCAAACTGAACGAAATCAACAAAGAACTCCAAGATCCTCAGAACCTCGGGCGGGCCCCTTTGCCATTG
  CTGCCCAACTTCGAATCCAAGCTGCACTCTCCAGACAGAAGTACCAGACCTTACTCGTTCCCCGAGTCCT
  TAGACCGATCGTTTAATGGACAACACTTGCAGGACTTGAGTTTTTTAACTCCCAGGGGAGAAGGCGGGAG
  TCCAGGACCGATCCGAAGTCCAGGACAGATCCGAAGTCCAGCCCCTCTAGATGAGTTTTCATTTGGTAGC
  TATGTGCCTTCGTTTGGAAAAACATTAGGGAAGTCAAATCCACCTAGCCAAAAAAGTAGCCTTTTAGATT
  TCCAAAGCAACAGTTCTGAAAGCCCGAGTAAAGACAGTCTTGATTTAATGTCGAGGAAAGAGAAAAAGGC
  GACTCTGATGGAGCAGCTGTTTGGCCCCAGCGCCAGCAACACCAGTGTCTCCTCCAAGAGCACCGACCCA
  CACTTCCCTGCTGCCAGCAGAGGTGACATGGACCCTCTTCATTTCCTCTCTGGGGACAGAAACAGCAGAG
  TCCGGGAGCCTGGCGATGAAGAGGAAGACCTTTTCCTCAGGGAAGGAAGAAGTTTTAATCCAAATAGACA
  CCGGTTGAAGCACGCAAGCAATAAGCCAACAGTCACGGCCGTTGACTCTGTTGATGAGGACATTGAAGAG
  GTTACCCTCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210147 representing BC052060
  Red=Cloning site Green=Tags(s)
  
  MGERARSPDIEQGKKGGKHPYSHYSSDLGSSPQSSGPSSPVNTTPCASTREKNPKRHLSDNQVHHPTVRK
  VSPKAVPSKKGIRVGFRSQSLNREPLRKDPDIVTKRVLSARLLKINELQNEVSELQVKLAQLLKENKALK
  SLQYRQEKALNKFEDAENEISQLIHRHNNEITALKERLRKSQEKERATEKRVKETEGELFRTKFSLQKLK
  KISEARHLPERDDLAKKLVSAELKLDDTERKIKELSKNLELSTNSFQRQLLAERKRAFEAYDENKVLQKE
  LQRLHHKLKEKEKELDIKNIYANRLPKSSPKKEKEIERKHVSCQSDFTDQCTKGVQTAEDFELEDFPFTA
  QTVLCYENRWDEPEYLSSYLEYQDLNKHGSEMLSSVLGQEGKYDEDEDPCSAKQEARKPESEWAREELDK
  VKGKSALLGRAEKLALEAGRFPTENYQAQSVDKFEDEAERLKTEMLLAKLNEINKELQDPQNLGRAPLPL
  LPNFESKLHSPDRSTRPYSFPESLDRSFNGQHLQDLSFLTPRGEGGSPGPIRSPGQIRSPAPLDEFSFGS
  YVPSFGKTLGKSNPPSQKSSLLDFQSNSSESPSKDSLDLMSRKEKKATLMEQLFGPSASNTSVSSKSTDP
  HFPAASRGDMDPLHFLSGDRNSRVREPGDEEEDLFLREGRSFNPNRHRLKHASNKPTVTAVDSVDEDIEE
  VTLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052060
• ORF Size: 2112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052060.1
• RefSeq Size: 4185
• RefSeq ORF: 2114
• Locus ID: 75782
• Gene Summary: Might be involved in minus end-directed microtubule transport. [UniProtKB/Swiss-Prot Function]