Ngef (BC039279) Mouse Tagged ORF Clone

CAT#: MG210191

Ngef (GFP-tagged) - Mouse neuronal guanine nucleotide exchange factor (cDNA clone MGC:37278 IMAGE:4973826)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ngef
• Synonyms: ephexin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210191 representing BC039279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAAAAACTCTGAAGACTGGGGGAAACCTCAGAGGAAATCAGAGAGCAGCTCACGGAAAAGCA
  ATCATGGCCCAGCTGAGATGAGGCCTGCGTTACCTCCGGAGAACAGGGAGGCACCTGAGACTGGCGAGGA
  AACTCAAAACGAAGAACCCCGCCGCCTCATCCCAATCCAGAGGCATTCCCTCTTCAATCGTGCTGTGAGA
  CACAGGCACAAGGCCAGGAGCACGTCTGAGCGGAGAGCCAGTGACCAAGCAGATTTGCCAAAAATGGGGA
  AATCTGTAAATGAACGGTCGGCCTTCAATCTGCCCCAGGGGAGATTGTCTCCCTGGAGAACACCAGCCCA
  GAGGGACACAGGAGCGCAGGAAGCCAGCGAGTCATCCTCCACCCCTGGAAATGGAACCACACCCGAGGAG
  TGCCCAGCCCTCACCGACAGCCCCACCACTCTCACGGAGGCCCTGCAGATGATCCATCCCATTCCCGCCG
  ACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAAGAGTATAGAGACAAATCGACTCTCCA
  AGAAATTGAAACACGGAGGCAGCAGGATGCAGAAATCCAAGGCAACTCCGATGGGTCCCAGGTTGGGGAA
  GACGCTGGAGAGGAGGAGGAGGAGGAGGAGGAGGGAGAGGAGGAGGAGCTGGCCAGCCCTCCTGAGAGGA
  GAGCTCTGCCTCAGATCTGCCTGCTCAGCAACCCCCACTCCAGGTTCAACCTCTGGCAAGACCTTCCTGA
  GATCCAGAGCAGTGGCGTGCTGGACATTCTCCAGCCGGAGGAGATCAGGCTGCAGGAGGCCATGTTTGAG
  TTGGTTACCTCTGAGGCCTCCTACTATAAGAGCCTGAACCTGCTGGTGTCGCACTTCATGGAGAACGAGC
  GTCTGAAGAAGATCCTGCATCCATCCGAGGCCCACATCCTCTTTTCCAATGTCCTGGATGTCATGGCTGT
  CAGTGAGCGGTTTTTGCTGGAGCTAGAGCACCGCATGGAGGAGAACATTGTTATCTCGGATGTGTGCGAC
  ATCGTGTACCGTTACGCAGCTGATCACTTCTCGGTCTATATCACTTACGTCAGTAACCAGACCTACCAGG
  AAAGGACATACAAGCAGCTCCTACAGGAGAAGGCCGCTTTCCGGGAACTGATCGCACAGTTGGAGCTGGA
  CCCCAAATGCAAGGGCCTGCCTTTCTCCTCCTTCCTCATCTTGCCTTTCCAGAGGATCACGAGACTCAAG
  CTGCTGGTCCAGAATATCCTGAAGAGAGTGGAGGAGAGGTCTGAACGTGAAGGCACCGCCTTGGATGCCC
  ACAAGGAGCTAGAAATGGTGGTAAAGGCATGCAATGAGGGTGTCCGGAAGATGAGCCGCACAGAACAGAT
  GATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTACCCATCATCTCACACTCCCGGTGGCTG
  CTGAAGCAGGGTGAGCTGCAGCAGATGTCCGGCCCCAAGACCTCCCGCACCCTGCGGACCAAGAAGCTCT
  TCAGAGAAATTTACCTCTTCCTCTTCAATGACCTGCTGGTGATCTGCCGGCAGATCCCTGGAGACAAGTA
  CCAGGTGTTTGATTCGGCCCCAAGGGGCCTGCTTCGAGTGGAGGAGCTGGAGGACCAGGGTCAAACACTG
  GCTAATGTGTTCATCCTGCGGCTGCTGGAAAATGCAGATGACCGAGAGGCCACCTATATGCTGAAGGCAT
  CCTCCCAGAGCGAGATGAAGCGCTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTTGTATCCTT
  CACATCTCGGCTGTTGGACTGTCCCCAGGTCCAGTGTGTGCACCCGTATGTGGCCCAGCAGCCTGATGAA
  CTGACGCTGGAACTGGCAGATATCCTGAACATCCTGGAGAAGACAGAGGATGGGTGGATCTTTGGTGAGC
  GGCTGCATGACCAGGAGAGAGGCTGGTTCCCCAGTTCCATGACAGAGGAGATCCTGAACCCCAAGATCCG
  CTCCCAGAACCTCAAGGAATGTTTCCGGGTACATAAGATGGAAGACCCTCAGCGCAGCCAGAATAAGGAC
  CGCAGGAAGCTGGGCAGCCGGAATCGTCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210191 representing BC039279
  Red=Cloning site Green=Tags(s)
  
  METKNSEDWGKPQRKSESSSRKSNHGPAEMRPALPPENREAPETGEETQNEEPRRLIPIQRHSLFNRAVR
  HRHKARSTSERRASDQADLPKMGKSVNERSAFNLPQGRLSPWRTPAQRDTGAQEASESSSTPGNGTTPEE
  CPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGNSDGSQVGE
  DAGEEEEEEEEGEEEELASPPERRALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFE
  LVTSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELEHRMEENIVISDVCD
  IVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCKGLPFSSFLILPFQRITRLK
  LLVQNILKRVEERSEREGTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWL
  LKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTL
  ANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDE
  LTLELADILNILEKTEDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMEDPQRSQNKD
  RRKLGSRNRQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC039279
• ORF Size: 2132 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC039279, AAH39279
• RefSeq Size: 3154
• RefSeq ORF: 2132
• Locus ID: 53972