Mark2 (BC058556) Mouse Tagged ORF Clone

CAT#: MG210289

Mark2 (GFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 2 (cDNA clone MGC:68226 IMAGE:5289799)

Product Images

Circular map for MG210289

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mark2
• Synonyms: Par-1, mKIAA4207
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >BC058556 ORF sequence, MG210289 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCAGCGCTCGGACCCCCCTACCCACGCTGAACGAAAGGGACACGGAGCAGCCCACCTTGGGACAC
  CTTGATTCCAAGCCCAGCAGTAAGTCCAACATGCTGCGGGGCCGCAACTCAGCCACCTCTGCTGACGAG
  CAGCCCCATATTGGCAACTACCGGCTCCTTAAGACCATTGGCAAGGGTAACTTTGCCAAGGTGAAGTTG
  GCCCGGCACATCCTGACGGGGAAAGAGGTAGCTGTGAAGATCATCGACAAGACCCAGCTGAACTCCTCC
  AGCCTACAGAAACTGTTCCGAGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTTAAGTTG
  TTTGAAGTGATCGAGACTGAGAAGACTCTCTACCTTGTCATGGAGTATGCCAGTGGCGGAGAGGTGTTT
  GATTACCTAGTGGCCCATGGCAGGATGAAAGAAAAAGAAGCTCGAGCCAAATTTCGCCAGATAGTGTCT
  GCTGTGCAGTACTGTCACCAGAAGTTCATTGTTCATAGAGATCTAAAGGCAGAAAACCTGCTCCTGGAT
  GCTGATATGAACATCAAGATTGCAGACTTTGGCTTTAGCAACGAATTCACCTTTGGGAACAAGCTGGAT
  ACTTTCTGTGGCAGTCCTCCTTATGCTGCCCCAGAACTTTTCCAGGGCAAAAAGTATGATGGTCCTGAG
  GTGGATGTCTGGAGCCTGGGAGTCATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAG
  AACCTCAAGGAGCTGCGGGAACGGGTACTGAGGGGGAAATACCGTATTCCGTTCTACATGTCCACGGAC
  TGTGAAAATCTGCTTAAGAAATTTCTCATACTTAATCCTAGTAAGAGAGGCACTTTAGAGCAAATTATG
  AAAGATCGGTGGATGAACGTGGGGCATGAGGACGATGAGCTAAAGCCTTATGTGGAACCTCTCCCTGAC
  TACAAGGACCCCCGGCGGACAGAGTTGATGGTGTCAATGGGTTACACACGGGAAGAGATCCAGGACTCG
  CTGGTAGGCCAGAGGTACAACGAAGTGATGGCTACCTATCTGCTCCTTGGCTACAAGAGCTCTGAGCTG
  GAAGGTGATACCATCACTTTGAAGCCCCGGCCTTCAGCTGATCTAACCAACAGCAGTGCCCCATCTCCA
  TCCCACAAGGTTCAGCGCAGCGTCTCTGCCAACCCCAAGCAACGACGCTCCAGTGACCAGGCCGTCCCT
  GCCATTCCCACCTCGAATTCCTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAATAAGCGGCCTGAG
  GAAGAGACAGGGCGGAAAGCCAGCAGCACCGCCAAAGTGCCTGCCAGCCCTCTGCCTGGCCTGGACAGG
  AAGAAGACCACTCCTGCCCCCTCCACGAACAGCGTCCTTTCCACCAGCACAAACCGAAGCAGGAACTCC
  CCACTTTTGGACAGGGCCAGCCTTGGCCAGGCCTCCATCCAGAATGGTAAAGACAGCACAGCCCCCCAG
  CGCGTCCCTGTCGCCTCCCCCTCCGCCCACAACATCAGCAGCAGTAGTGGAGCCCCAGACCGAACTAAT
  TTCCCACGGGGTGTGTCCAGTCGAAGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAG
  AATCTACCCTACGGTGTGACCCCAGCCTCTCCCTCTGGCCATAGCCAGGGCCGGCGGGGGGCCTCTGGC
  AGCATCTTCAGCAAGTTCACCTCCAAGTTTGTCCGCAGGAACCTGAATGAACCTGAAAGCAAAGACCGA
  GTGGAGACGCTCAGACCTCACGTGGTAGGCAGTGGAGGCACTGACAAGGACAAGGAGGAGTTTCGGGAG
  GCCAAGCCTCGCTCCCTGCGCTTCACCTGGAGCATGAAGACCACGAGCTCTATGGAGCCCAATGAGATG
  ATGCGGGAGATCCGCAAGGTGCTGGACGCCAACAGCTGCCAAAGCGAGCTGCACGAGCGGTACATGCTA
  CTGTGCGTGCATGGCACACCAGGCCACGAGAACTTTGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCC
  CGGCTGTCTCTCAACGGTGTTCGGTTTAAGCGGATATCGGGCACTTCCATGGCCTTCAAAAACATTGCC
  TCCAAAATAGCCAATGAGCTGAAGCTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by MG210289
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMLRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKL
  ARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVF
  DYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLD
  TFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTD
  CENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDS
  LVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRSSDQAVP
  AIPTSNSYSKKTQSNNAENKRPEEETGRKASSTAKVPASPLPGLDRKKTTPAPSTNSVLSTSTNRSRNS
  PLLDRASLGQASIQNGKDSTAPQRVPVASPSAHNISSSSGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQ
  NLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLNEPESKDRVETLRPHVVGSGGTDKDKEEFRE
  AKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHERYMLLCVHGTPGHENFVQWEMEVCKLP
  RLSLNGVRFKRISGTSMAFKNIASKIANELKL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058556
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3106 bp
• RefSeq ORF: 2168 bp
• Locus ID: 13728
• Cytogenetics: 19 5.32 cM
• MW: 80.9 kDa
• Gene Summary: Serine/threonine-protein kinase. Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.[UniProtKB/Swiss-Prot Function]