Smurf1 (BC029097) Mouse Tagged ORF Clone

CAT#: MG210325

Smurf1 (GFP-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (cDNA clone MGC:28022 IMAGE:3660965)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smurf1
• Synonyms: mKIAA1625
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210325 representing BC029097
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTATTGTGTGCCAAGA
  ACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAGATTGTTGTGGACGGCTCTGGGCA
  GTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGAGCCAGCACTATGACCTGTATGTT
  GGGAAAACGGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGATCCACAAGAAGCAGGGGGCTGGCT
  TCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAAGACACTGGCTACCAGCGTTTGGA
  TCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAATAGTGGTCAGTTTACAGACCCGA
  GACAGAATAGGCGGTGGAGGGTCAGTGGTGGACTGCAGAGGGCTGCTGGAGAACGAAGGAACAGTGTATG
  AAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCTGCCCCGTATACAGATGGTACTGG
  TGCAGCAGCAGGAGGCGGGAACTGCAGGTTTGTGGAGTCTCCAAGCCAAGATCAGAGACTCCTGGTACAG
  CGACTCCGAAATCCTGAGGTTCGAGGGCCCTTACAGACACCCCAGAACCGACCACATGGCCACCAGTCGC
  CAGAGCTGCCTGAAGGCTATGAGCAAAGGACAACAGTGCAGGGACAAGTTTACTTTTTGCACACGCAGAC
  TGGAGTCAGCACATGGCATGACCCCAGGATCCCCAGAGACCTTAACAGTGTGAACTGCGATGAACTTGGG
  CCACTGCCTCCAGGCTGGGAAGTCCGAAGCACAGTGTCGGGAAGAATCTATTTTGTAGATCACAATAATA
  GGACAACCCAGTTTACAGATCCACGGCTTCACCACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAG
  CCAGCCGCTGCAGCTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGCTTCCTGCCCAGAGATATGAGAGG
  GACTTAGTCCAGAAGCTCAAAGTTCTCAGGCACGAGCTCTCTCTTCAGCAGCCCCAGGCTGGTCACTGTC
  GAATAGAAGTCTCCAGAGAAGAGATATTTGAGGAGTCGTATCGCCAGATCATGAAGATGCGGCCAAAAGA
  CCTAAAGAAGCGCCTCATGGTGAAGTTCCGAGGGGAGGAAGGTTTGGACTATGGTGGAGTGGCTCGGGAG
  TGGCTGTATTTGTTGTGCCATGAAATGTTGAACCCGTACTATGGACTCTTCCAGTATTCCACGGACAATA
  TTTACACACTGCAGATCAACCCAGATTCTTCTATCAACCCTGACCATCTGTCTTACTTCCACTTTGTGGG
  TCGCATCATGGGTCTGGCTGTGTTCCACGGACACTACATAAATGGGGGTTTCACAGTTCCGTTCTACAAG
  CAGCTCCTGGGGAAGCCAATCCAGCTGTCGGACCTGGAGTCCGTGGACCCAGAACTGCACAAGAGCTTGG
  TGTGGATTCTAGAGAATGACATCACGCCTGTGTTGGATCATACCTTCTGCGTGGAGCACAACGCTTTCGG
  GCGGATTCTCCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTGTCACTGAGGAGAACAAGAAGGAA
  TACGTCCGGCTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGCCCAGTTCTTAGCACTTCAGAAGG
  GGTTTAACGAACTCATTCCCCAACACTTGCTGAAGCCCTTTGACCAGAAGGAACTAGAGCTGATAATAGG
  TGGGCTGGATAAGATAGACCTGAACGACTGGAAGTCCAACACCCGGCTGAAACACTGTGTGGCAGACAGC
  AACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGAGAGGAGAGCCAGACTCCTGCAGT
  TTGTGACAGGATCCACAAGAGTTCCTCTCCAAGGCTTCAAGGCTCTGCAAGGCGCGGCAGGGCCCCGGCT
  GTTCACCATTCACCTGATAGACGCCAATACAGACAACCTGCCCAAGGCCCATACCTGCTTTAATCGGATC
  GACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCTGACAGCGGTGGAGGAAACCTGTG
  GCTTTGCAGTGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210325 representing BC029097
  Red=Cloning site Green=Tags(s)
  
  MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWSQHYDLYV
  GKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTR
  DRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQ
  RLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELG
  PLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYER
  DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVARE
  WLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYK
  QLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKE
  YVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADS
  NIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRI
  DIPPYESYEKLYEKLLTAVEETCGFAVE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC029097
• ORF Size: 2186 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC029097, AAH29097
• RefSeq Size: 2765
• RefSeq ORF: 2186
• Locus ID: 75788