Polk (BC052820) Mouse Tagged ORF Clone

CAT#: MG210382

Polk (GFP-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polk
• Synonyms: Dinb1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210382 representing BC052820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAACACAAAGGAAAAGGACAACTTCAAAGACGACCTCCTGCTCCGCATGGGACTAAACGATAACA
  AAGCAGGCATGGAAGGGTTGGATAAGGAGAAAATTAACAAAATTATCATGGAAGCCACAAAGGGGTCCAG
  ATTTTATGAATATGATCCCAATTTTATGGCCATGAGTCTGGACGAAGCCTACTTGAATATAACACAGCAC
  TTGCAGGAAAGGCAAGATTGGCCTGAGGACAAAAGAAGATACTTCATCAAAATGGGAAACTACTTAAAAA
  TCGACACACCCAGACAGGAAGCTAACGAGCTGACTGAGTATGAGCGGTCCATCTCCCCGCTGCTTTTTGA
  AGATAGTCCTCCTGATTTGCAACCCCAAGGAAGTCCTTTCCAACTGAACTCTGAAGAACAAAACAATCCT
  CAAATAGCCCAAAATTCAGTTGTTTTTGGAACATCAGCTGAGGAAGTGGTAAAGGAAATTCGCTTCAGAA
  TTGAACAAAAAACAACGCTGACAGCCAGCGCAGGCATCGCCCCCAATACAATGTTAGCAAAAGTGTGCAG
  TGATAAGAATAAGCCAAACGGACAGTACCAGATCCTTCCCAGCAGGAGCGCGGTGATGGACTTCATCAAG
  GACCTGCCTATTAGAAAGGTTTCTGGGATAGGAAAAGTTACAGAGAAAATGTTAATGGCTCTCGGGATTG
  TTACTTGCACAGAACTCTACCAACAGAGAGCGTTGCTGTCTCTCCTTTTCTCTGAAACCTCTTGGCATTA
  TTTTCTTCACATCGCGCTGGGTCTAGGTTCAACAGACCTGGCAAGGGATGGAGAAAGGAAAAGCATGAGT
  GTTGAAAGGACATTCAGTGAGATAAGTAAGACAGAGGAACAGTACAGCCTGTGCCAAGAACTGTGCGCTG
  AGCTCGCCCACGACCTCCAGAAGGAAGGACTTAAGGGAAGAACCGTCACCATTAAGCTGAAGAACGTGAA
  TTTTGAAGTAAAAACTCGTGCATCTACCGTTCCGGCCGCCATTTCTACTGCAGAGGAAATATTTGCCATT
  GCCAAGGAGCTGCTAAGGACAGAAGTTAATGTGGGTTCTCCACACCCCCTGCGGTTAAGACTGATGGGTG
  TCCGAATGTCTACTTTTTCCAGTGAAGATGACAGGAAACACCAACAAAGGAGCATCATTGGTTTCTTACA
  AGCTGGAAACCAAGCTTTGTCATCTACTGGGGGTAGTCTAGACAAAACTGCCAAAACTGAGCTTGCAAAG
  CCCTTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAGCGATCAGAAAGAATCTCCAACTGTCAAG
  ACACATCCAGATGTAAAACTGCGGGTCAGCAAGCTTTACAGATCTTGGAACCATCCCAAGCATTAAAGAA
  GCTGAGCCAGAGTTTTGAAACATCAGAGAATTCAAATGACTGTCAGACATTTATATGTCCAGTTTGCTTT
  AGGGAGCAAGAAGGTGTCAGTCTGGAAGCCTTTAATGAACATGTAGATGAGTGTCTTGATGGACCGTCAA
  CCAGTGAGAACTCAAAAATATCCTGTTACTCACATGCTTCCTCTGCAGACATTGGTCAGAAGGAAGATGT
  ACACCCCTCTATTCCACTGTGTGAGAAACGGGGGCATGAAAATGGAGAGATCACTTCAGTAGATGGTGTA
  GATCTAACAGGGACGGAAGACAGATCATTGAAAGCAGCAAGTATGGACACTCTAGAGAATAATCGCAGCA
  AAGAGGAATGTCCTGATATTCCAGACAAGTCTTGTCCTATATCACTGGCAAATGAAACCATCAGTACATT
  AAGTAGGCAAGAATCTGTCCAGCCTTGTACAGATGAGGTAGTAACAGGACGAGCTCTAGTGTGTCCTGTT
  TGTAACCTAGAACAAGAGACTTCTGATCTTACCCTCTTCAACATACATGTGGATATTTGCTTAAATAAAG
  GTATTATCCAAGAACTGAGAAATAGTGAAGGTAATTCAGTTAAACAACCCAAAGAAAGCTCGAGAAGTAC
  TGACAGACTTCAGAAGGCTTCAGGAAGGACCAAAAGGCCACGTGGAGCAACCTCACCATCAGCTCCTCCA
  TGCTTAGCCACCATGGTACCATTGATGCTTCCGTACCTACCTGTGTGCATCTCTGTGTCCTGTGAGCAGA
  TGCAGTTAGAATTGGCCATGATGTCCATGAAGAACATTAGCTTAACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210382 representing BC052820
  Red=Cloning site Green=Tags(s)
  
  MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYEYDPNFMAMSLDEAYLNITQH
  LQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNP
  QIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIK
  DLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMS
  VERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAI
  AKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGGSLDKTAKTELAK
  PLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSQSFETSENSNDCQTFICPVCF
  REQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITSVDGV
  DLTGTEDRSLKAASMDTLENNRSKEECPDIPDKSCPISLANETISTLSRQESVQPCTDEVVTGRALVCPV
  CNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPRGATSPSAPP
  CLATMVPLMLPYLPVCISVSCEQMQLELAMMSMKNISLT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052820
• ORF Size: 2219 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052820, AAH52820
• RefSeq Size: 3517
• RefSeq ORF: 2219
• Locus ID: 27015