Ezh1 (BC007135) Mouse Tagged ORF Clone

CAT#: MG210461

Ezh1 (GFP-tagged) - Mouse enhancer of zeste homolog 1 (Drosophila) (cDNA clone MGC:5934 IMAGE:3600884)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ezh1
• Synonyms: mKIAA0388
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210461 representing BC007135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAAATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAAGTGAAAT
  CTGAGTATATGCGGCTTCGACAGCTCAAACGGCTCCAGGCAAATATGGGAGCAAAGGCTCTGTATGTGGC
  AAATTTTGCAAAGGTTCAAGAAAAAACCCAAATCCTCAATGAAGAGTGGAAGAAACTTCGTGTCCAGCCT
  GTTCAGCCAATGAAGCCCGTGAGTGGGCACCCTTTTCTGAAAAAGTGTACCATAGAGAGCATTTTCCCAG
  GGTTCGACAGCCAGGATATGTTGATGCGGTCTCTGAACACTGTTGCACTGGTTCCCATCATGTATTCCTG
  GTCCCCTCTCCAGCAGAATTTCATGGTGGAAGATGAGACGGTTTTGTGCAATATTCCATACATGGGTGAC
  GAGGTGAAGGAAGAAGATGAGACTTTCATCGAAGAGCTGATCAATAACTATGATGGCAAAGTCCACGGTG
  AAGAAGAGATGATCCCTGGATCTGTGCTGATCAGCGATGCTGTGTTTCTGGAGCTGGTGGATGCCCTCAA
  CCAGTACTCTGATGAGGAGGAGGACGGGCACAACGACCCCTCCGATGGAAAGCAAGACGACAGCAAAGAG
  GACCTGCCGGTAACAAGAAAACGGAAGCGCCATGCTATCGAAGGCAACAAAAAGAGTTCCAAGAAACAGT
  TTCCAAATGACATGATCTTCAGCGCCATTGCGTCCATGTTTCCTGAGAATGGTGTCCCTGACGACATGAA
  GGAGAGGTATCGAGAGCTGACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATC
  GATGGCCCCAACGCCAAGTCAGTGCAGCGGGAGCAGTCTCTGCACTCTTTCCACACCCTTTTCTGCCGGC
  GCTGTTTTAAATATGACTGCTTCCTTCACCCTTTCCACGCCACCCCAAATGTATATAAGCGCAAGAACAA
  GGAAATCAAGATTGAGCCAGAACCGTGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTAC
  GCCATGCTGCACAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTT
  CCTGCTCCAATGCATCAGCTTCTGCTATGGCTGAAACTAAAGAAGGAGACAGTGATAGAGACACTGGCAA
  TGACTGGGCCTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACCCCCACGAAACAGAAAGCCAGTCCA
  GCCCCAGCTCAGCTCTGTGTTGTGGAAGCCCCCTCAGAGCCGGTGGAATGGACCGGAGCCGAAGAATCTC
  TTTTCCGAGTCTTCCACGGCACCTATTTCAACAACTTCTGCTCAATAGCCAGGCTTCTGGGGACAAAGAC
  ATGCAAGCAGGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTAAAGCTGCCAACAGATGAGCTCATG
  AACCCTGCACAGAAGAAGAAAAGAAAACACAGGTTGTGGGCCGCACACTGCAGGAAAATTCAGCTGAAGA
  AAGATAACAATTCTACACAAGTGTATAACTACCAACCCTGTGACCACCCAGACCGTCCGTGTGACAGCAC
  ATGCCCCTGCATCATGACCCAGAACTTTTGTGAAAAGTTCTGCCAGTGCAGCCCAGACTGCCAGAATCGC
  TTTCCTGGTTGTCGCTGTAAGACTCAGTGCAATACCAAGCAATGTCCATGCTACTTGGCAGTTCGTGAGT
  GTGACCCTGACTTGTGCCTCACCTGTGGGGCCTCAGAGCACTGGGACTGTAAGGTGGTGTCTTGCAAAAA
  CTGCAGCATCCAGCGTGGCCTCAAAAAGCACCTGCTGCTGGCCCCTTCCGATGTGGCCGGATGGGGCACC
  TTCATCAAGGAGTCTGTGCAGAAGAATGAATTCATTTCTGAATATTGTGGTGAGCTCATCTCTCAGGATG
  AGGCTGATCGTCGAGGGAAGGTCTATGATAAATACATGTCTAGCTTCCTCTTCAACCTCAACAATGATTT
  TGTAGTGGATGCTACCCGGAAAGGAAACAAAATTCGCTTTGCAAACCATTCAGTGAACCCCAACTGTTAT
  GCCAAAGTGGTTATGGTGAATGGAGATCACCGCATTGGGATCTTTGCCAAGAGAGCAATTCAGGCTGGCG
  AAGAGCTCTTCTTTGATTATAGGTACAGCCAAGCTGATGCCCTCAAGTATGTGGGCATCGAGAGGGAAAC
  GGACGTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210461 representing BC007135
  Red=Cloning site Green=Tags(s)
  
  MRKMDIASPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQP
  VQPMKPVSGHPFLKKCTIESIFPGFDSQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGD
  EVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEDGHNDPSDGKQDDSKE
  DLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNI
  DGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEY
  AMLHNPRSKCSGRRRRRHPVVSASCSNASASAMAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASP
  APAQLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELM
  NPAQKKKRKHRLWAAHCRKIQLKKDNNSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNR
  FPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGT
  FIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCY
  AKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC007135
• ORF Size: 2252 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC007135, AAH07135
• RefSeq Size: 4179
• RefSeq ORF: 2252
• Locus ID: 14055
• Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. The encoded protein is interchangeable with the related Enhancer of zeste 2 (Ezh2) protein as a core component of the polycomb repressive complex 2 (PRC2), which methylates histone H3 at lysine 27 and results in the transcriptional repression of affected target genes. This complex is involved in carrying out cell-fate decisions during embryonic stem cell differentiation. [provided by RefSeq, Sep 2014]