Pfkl (NM_008826) Mouse Tagged ORF Clone

CAT#: MG210614

Pfkl (GFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pfkl
• Synonyms: AA407869; ATP-PFK; PFK-B; PFK-L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210614 representing NM_008826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCGTGGACCTGGAGAAACTGCGGATGTCGGGGGCTGGCAAGGCCATTGGAGTGCTGACCAGCG
  GCGGTGATGCGCAAGGTATGAATGCTGCTGTCAGGGCTGTGACCCGTATGGGCATATATGTGGGGGCCAA
  AGTCTTCCTCATCTATGAGGGCTACGAGGGCCTTGTGGAAGGAGGCGAGAACATCAAGCCAGCCAACTGG
  CTCAGCGTTTCCAATATCATCCAGCTGGGTGGCACCATTATTGGCAGTGCCCGCTGTAAGGCCTTCACTA
  CGAGGGAAGGCCGCCTGGCTGCAGCCTACAATCTGCTCCAACACGGCATCACCAACCTGTGTGTCATCGG
  TGGCGATGGCAGCCTCACGGGGGCCAACATCTTCCGCAACGAGTGGGGCAGCTTGCTGGAGGAGCTGGTG
  AAGGAAGGCAAGATCTCAGAGTCCACAGCTCAGAACTACGCACACTTGACCATCGCCGGTCTGGTGGGCT
  CCATCGATAATGACTTCTGCGGCACTGACATGACCATTGGCACAGACTCAGCCCTGCACCGCATTATGGA
  GGTCATTGACGCCATCACTACCACTGCCCAAAGTCACCAGAGGACCTTTGTTTTGGAGGTGATGGGACGG
  CACTGCGGGTACCTGGCGCTGGTGTCTGCGCTGGCTTCCGGGGCTGATTGGCTATTCATTCCTGAAGCGC
  CCCCTGAGGATGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGCGAGACTCGGAGCCGAGGCTCTCGGCT
  GAACATCATCATCATCGCAGAGGGCGCCATTGACCGGCATGGAAAGCCTATCTCATCCAGCTACGTGAAG
  GATCTGGTGGTTCAGAGGCTGGGCTTCGATACACGAGTGACTGTGCTGGGTCATGTACAGCGAGGAGGGA
  CGCCTTCAGCCTTCGACCGAATCCTGAGTAGCAAGATGGGTATGGAGGCCGTGATGGCGCTGCTAGAGGC
  CACGCCTGACACGCCGGCCTGTGTGGTCAGCCTCTCCGGGAACCAGTCTGTGAGGCTGCCTCTCATGGAG
  TGTGTGCAAGTGACAAAGGACGTGCAGAAGGCCATGGACGAGGAGAGGTTTGACGAGGCCATCCAGCTCC
  GTGGCAGGAGCTTTGAGAACAACTGGAAAATTTACAAGCTCCTTGCCCACCAGAAGGTGTCTAAAGAGAA
  GTCCAACTTCTCCCTGGCCATCCTGAATGTGGGGGCTCCAGCTGCTGGCATGAATGCAGCTGTGCGCTCC
  GCAGTGCGCACCGGTATCTCCGAGGGACACACGGTATACATCGTGCATGATGGCTTTGAGGGTCTGGCCA
  AGGGTCAGGTGCAAGAAGTGGGCTGGCATGATGTGGCAGGCTGGCTGGGACGTGGTGGCTCGATGCTGGG
  GACCAAGAGGACACTGCCCAAGCCCCATCTGGAGGCCATTGTGGAGAATCTCCGCACCTACAACATCCAC
  GCCCTGCTGGTGATTGGTGGCTTTGAGGCCTACGAGGGTGTGCTGCAGCTGGTGGAGGCCCGGGGGCGCT
  ACGAGGAACTCTGCATTGTCATGTGCGTCATCCCAGCCACCATCAGCAACAATGTGCCTGGCACTGACTT
  CAGCCTGGGCTCAGACACGGCTGTCAACGCTGCAATGGAGAGTTGTGATCGCATCAAGCAGTCAGCCTCA
  GGGACAAAGCGGCGTGTGTTCATTGTAGAGACCATGGGGGGCTACTGTGGCTACCTGGCCACTGTGACCG
  GCATTGCTGTGGGTGCCGATGCTGCCTACGTCTTTGAGGACCCTTTCAACATCCATGACTTAAAGGCCAA
  TGTGGAGCATATGACAGAGAAGATGAAGACAGACATCCAGAGGGGACTGGTGCTCCGGAACGAGAAGTGT
  CACGAACACTACACCACAGAGTTCCTATACAACCTGTACTCCTCAGAAGGCAGGGGCGTGTTTGACTGCA
  GGACCAATGTGCTGGGCCACTTACAGCAGGGTGGTGCTCCAACCCCTTTCGACCGGAACTATGGGACCAA
  ACTGGGGGTGAAGGCCATGTTGTGGGTGTCTGAGAAGCTACGTGATGTCTACCGTAAAGGGCGGGTGTTT
  GCCAATGCTCCAGACTCAGCCTGTGTGATCGGCCTGCGGAAGAAGGTAGTGGCCTTCAGTCCGGTCACAG
  AACTCAAGAAAGAGACTGATTTTGAGCACCGCATGCCCCGGGAGCAGTGGTGGCTGAATCTGCGGCTGAT
  GCTGAAGATGCTGGCACACTATCGCATCAGCATGGCAGACTATGTGTCTGGGGAGCTGGAGCACGTCACA
  CGCCGCACCTTGAGCATAGACAAGGGTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210614 representing NM_008826
  Red=Cloning site Green=Tags(s)
  
  MATVDLEKLRMSGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKPANW
  LSVSNIIQLGGTIIGSARCKAFTTREGRLAAAYNLLQHGITNLCVIGGDGSLTGANIFRNEWGSLLEELV
  KEGKISESTAQNYAHLTIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGR
  HCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHGKPISSSYVK
  DLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVSLSGNQSVRLPLME
  CVQVTKDVQKAMDEERFDEAIQLRGRSFENNWKIYKLLAHQKVSKEKSNFSLAILNVGAPAAGMNAAVRS
  AVRTGISEGHTVYIVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKPHLEAIVENLRTYNIH
  ALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSAS
  GTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKC
  HEHYTTEFLYNLYSSEGRGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWVSEKLRDVYRKGRVF
  ANAPDSACVIGLRKKVVAFSPVTELKKETDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVT
  RRTLSIDKGF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008826
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008826.2, NP_032852.2
• RefSeq Size: 3350
• RefSeq ORF: 2343
• Locus ID: 18641
• Gene Summary: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProtKB/Swiss-Prot Function]