Tle3 (BC056465) Mouse Tagged ORF Clone

CAT#: MG210620

Tle3 (GFP-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (cDNA clone MGC:67232 IMAGE:5709252)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tle3
• Synonyms: Grg3a, Grg3b, ESG
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210620 representing BC056465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCGCAAGGCAGACATCCGGCACCCCATCAACCCGGGCAGCCGGGATTTAAATTCACTGTGGCCG
  AGTCCTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA
  TGACAAGCTGGCTAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC
  TTGAATATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATCCTAGCCCAGATCATGC
  CTTTTTTGTCACAGGAGCATCAGCAGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGAC
  GGAGTTGAACGCCATCATCGGGGTACGTGGACTCCCCAATCTGCCTCTCACCCAGCAGCAACTCCAGGCC
  CAGCACCTCTCCCATGCCACGCATGGTCCCCCGGTCCAGCTGCCACCCCACCCGTCAGGCCTCCAGCCTC
  CTGGGATTCCCCCAGTGACAGGAAGCAGCTCTGGGTTGCTGGCACTTGGTGCCCTGGGAAGTCAAGCTCA
  CTTGGCGGTGAAGGATGAGAAGAACCACCATGAACTGGATCACAGAGAGAGAGAGTCCAGCACGAACAAT
  TCCGTGTCACCCTCTGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCTGCAGACTACAGCATGGAAG
  CCAAGAAGCGGAAGGCGGAAGAGAAAGACAGCCTCAGCAGATACGATAGCGATGGGGACAAGAGTGACGA
  CCTGGTGGTGGATGTCTCTAATGAGGACCCAGCAACACCCCGGGTGAGCCCAGCACACTCCCCTCCTGAA
  AATGGGCTGGACAAAGCCCGTGGTCTGAAGAAAGATGCCCCCACCAGCCCAGCCTCCGTGGCTTCCTCCA
  GCAGCACACCTTCCTCCAAGACCAAAGACCTTGGTCATAATGACAAATCTTCCACACCTGGGCTCAAGTC
  CAACACACCAACGCCAAGAAATGATGCCCCAACTCCAGGCACCAGCACCACCCCGGGACTCCGGTCAATG
  CCGGGCAAACCTCCAGGCATGGACCCGATAGGTATAATGGCCTCGGCCCTGCGAACACCCATCACCCTCA
  CCAGCTCCTATCCAGCACCCTTTGCCATGATGAGCCACCACGAGATGAATGGCTCCCTCACCAGCCCAAG
  CGCCTATGCTGGCCTACACAACATCCCATCCCAGATGAGCGCCGCCGCAGCCGCTGCAGCCGCCGCCTAT
  GGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTTGACCCTCACCCCCCAATGAGGGCCACAGGCC
  TGCCTTCCAGTCTCGCCTCCATTCCTGGAGGGAAACCGGCATACTCCTTCCATGTGAGTGCTGATGGGCA
  GATGCAACCTGTGCCCTTCCCCCATGATGCACTAGCAGGCCCTGGCATTCCCAGGCATGCCCGGCAGATC
  AATACGCTCAGCCATGGAGAGGTGGTATGTGCTGTGACCATCAGCAACCCCACACGACACGTCTACACAG
  GCGGCAAGGGCTGTGTGAAGATATGGGACATCAGCCAGCCGGGCAGCAAGAGTCCCATCTCCCAGCTGGA
  CTGCCTGAACAGGGACAACTACATCCGCTCGTGCAAGCTTCTCCCCGATGGGCGCACGCTCATTGTGGGT
  GGTGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCACCCACACCCCGCATCAAGGCTGAGCTGACGT
  CCTCGGCTCCAGCCTGTTATGCCCTGGCCATCAGTCCTGATGCCAAAGTCTGTTTTTCCTGCTGCAGCGA
  CGGGAACATTGCGGTTTGGGATCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGG
  GCCAGCTGTATAGACATCTCTCATGATGGCACTAAGCTGTGGACCGGGGGCCTGGACAACACCGTGCGCT
  CCTGGGACCTACGTGAAGGACGGCAGTTACAGCAACACGATTTCACCTCCCAGATCTTCTCCCTGGGTTA
  CTGCCCCACTGGGGAGTGGCTGGCCGTGGGCATGGAGAGCAGCAATGTGGAGGTCCTGCACCACACTAAG
  CCCGACAAATACCAGCTGCACCTGCACGAGAGCTGCGTGCTGTCCCTCAAGTTCGCCTATTGTGGCAAGT
  GGTTTGTGAGCACTGGGAAAGACAACCTTCTCAATGCCTGGAGGACGCCTTATGGAGCCAGCATCTTCCA
  GTCAAAAGAATCCTCATCTGTCTTGAGCTGTGACATTTCAGCGGATGACAAATATATTGTAACAGGCTCT
  GGTGACAAGAAGGCCACAGTTTACGAGGTCATCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210620 representing BC056465
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG
  LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQQQLQA
  QHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLAVKDEKNHHELDHRERESSTNN
  SVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPE
  NGLDKARGLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSM
  PGKPPGMDPIGIMASALRTPITLTSSYPAPFAMMSHHEMNGSLTSPSAYAGLHNIPSQMSAAAAAAAAAY
  GRSPMVSFGAVGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQI
  NTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVG
  GEASTLTIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDG
  ASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTK
  PDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGS
  GDKKATVYEVIY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC056465
• ORF Size: 2348 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC056465, AAH56465
• RefSeq Size: 3546
• RefSeq ORF: 2348
• Locus ID: 21887