Bank1 (NM_001033350) Mouse Tagged ORF Clone

CAT#: MG210629

Bank1 (GFP-tagged) - Mouse B-cell scaffold protein with ankyrin repeats 1 (Bank1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bank1
• Synonyms: A530094C12Rik; AI451642; AVIEF; BANK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210629 representing NM_001033350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCTGTGGCTTCTGGCACTAGGGGTAGCACCCAGGATCTGTTCCAGGTTGGCCTAGCACCTCCAG
  GAAATGCAAAAGATATCCTGTTGCTACATGAAGAAGATGCTGAGGAATGGGCTCTATACTTGAGAGAAAT
  ATTTATGCGTGTTGTAGAAAGAGAAGCAATCCTGTTATATCCCTTGCACAGTTTTTCTTCTTCACATTTG
  GAAATGCTAAACTTATGTGCTTACAAATGTAAACTTCTGATAATATCCAATAGTCTACTTAAAGACCTGA
  CGCCAAAGAAGTGTCAGTTTCTGGAGAAGATACTTCACTCAACAGGGAATGTAGTGACTTTGCTCTGTGG
  GATGGAGAGCTCAGATCCTTTCTACCAGTTATTAAGTATCCCCAGAAAGAGATGGGAGATCTCAACAGAG
  CAGGATCCGGATGGTTACATCTCTGTCATCAGACAGATCTTAGACCAAGGTCCTGAAGACTACCTTGAGG
  TCAGCATTCCAACAGACTCAAGAGCCAAGTATCCTGAGGACACAAGTGGACAGAAGGGAACTGACGTCCT
  AGCATCTCTGAGACCATCTGTGCCTCGGGTACTAGTGCTTCCTGGGGAAATTCCATGTGAGAAACCTGGT
  GAGATATTCATTCTGTTGAAAGATGAACTAATTGGCGAAATTCTAGAGGTTGAATTTATATCAACCAACA
  AGCGCCTCAGAGCACGGCCAGCACGTTGGAATAAGAGTGTCTGGCATATGAAAGCTGCAGATTTTCCAGC
  TGGCTCGGTCACTGTCAATATCCACTGTGATGGAATCATCAAGGCCACAACAGAGATTAAATACTGTTCA
  GCAGCAAAAGCAACAGAAAGTCCATTTAGAGTGTCAGACCCGGGCAAGAGTTTGTGCCAGAAAAGCGTCG
  AAGAACTCGATAATGTTCTTGCATCTATATTCAAGCGTGAGATACCATATTATGAATTCAAACATCTCCA
  AGCTGAAACTTACCCTCAAAAAGAACGTACTCACACCACAGAGCTCCCAACACTTCTTCACTGTGCAGCA
  AAATTTGGCTTAAAGAATCTGGCTCTTCATCTGCTGCAGTGTTCAGGAGCAACCAGGGCAGCTAGAATGA
  AGATGACAGATGGTTCAGACCTGCTGCATATTGCTGAAAGGCATGGTCATGAAGAACTCAAGGAAGTCTT
  TGAAGACTTTCTCAGCCAAAACACTGGCAGAAATAGCAAGCAAGAAAATGACTATGAAGAAGATGTAATC
  TCATTTTCCACATATTCACCCTCCATGCCGTCTCCGGCATCCCTTCATGAACTCAGGAAGACACACAGGC
  GGAACACAGACAGATCTGAGGAGCCTGAAAGGTCTGTGGAGATGAAGGAGGAAGAAGCAGGTGCTGAGGC
  AAGACGCAGCCTGTCAGAGGGCGAAAGGGAAAGCTCCGAGAACCAGTATGACGATCTGTATGTTTTCATC
  CCTGGGTTTGACACCGAAGGCAACTCTGAAGAGCCTCTCCCACACTGCAGGCCACCTCTGCTGCCACCAC
  GACCAGGCACTGCTGCCTCCCAGCTAGAAAGACCTCACTTTACCTCACAAGGAAAAGTACTGGAAGACCA
  AATGGAAAGAAGTCAAAACTGGAATGATCTCAATGCAAGACCAGAGACAAGAGAGGAATCCAGCAGAGAA
  GAAAAGAAAGAAGAAGCCCAGGAGGAGGAGGAAGAAGAAGAAAACCCATATGCATTTGCAGAGACTGAAG
  ACAATGAGTATGACCTGATACTGGCCAGTAAGAGTGTCAAGAAAAGAACTGGAAATCGGTCTTTCATTAT
  AAACAGACCACCGGCTCCCACACCCCGGCCCACGCACATCCCTCCCAAAGAAGAAACAACACCTTACATA
  GCTCAAGTGTTCCAACAAAAGGCAGCCAGAAGACAATCTGATGGTGATAAGTTCTACAGTCTCCCTAAGA
  AACCCGACAAAACTCGGATGGAGGGCCCAACCTTTCCTAGTACAAGGGATTATCTGACTACTGGGCAGGA
  AGAACTGATCCTCCTGCAGGAGAGAGTCAAGAATGGGAAAATGTCTGTGGATGAAGCTCTAGAGAAATTT
  AAACATTGGCAGATGGGAAAGAGTGGTCTGGAAATGATTCAGCAGGAAAAGCTACGGCAACTACGAGACA
  ACATTATTGGGAAAAGGCCAGAAGATGAAAATGCCTATGATAAACTGACCATTGTGCACCATCCGAGTGG
  TAATACTGCCCACAATGAAAATATGTTGTACAACAGTCCATTCAACAGTAAGTTTCCTGCTCGAATCCAA
  GTTGAAAAGGAGTTTGGTTTCTGCTGCAAAAAAGATCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210629 representing NM_001033350
  Red=Cloning site Green=Tags(s)
  
  MLPVASGTRGSTQDLFQVGLAPPGNAKDILLLHEEDAEEWALYLREIFMRVVEREAILLYPLHSFSSSHL
  EMLNLCAYKCKLLIISNSLLKDLTPKKCQFLEKILHSTGNVVTLLCGMESSDPFYQLLSIPRKRWEISTE
  QDPDGYISVIRQILDQGPEDYLEVSIPTDSRAKYPEDTSGQKGTDVLASLRPSVPRVLVLPGEIPCEKPG
  EIFILLKDELIGEILEVEFISTNKRLRARPARWNKSVWHMKAADFPAGSVTVNIHCDGIIKATTEIKYCS
  AAKATESPFRVSDPGKSLCQKSVEELDNVLASIFKREIPYYEFKHLQAETYPQKERTHTTELPTLLHCAA
  KFGLKNLALHLLQCSGATRAARMKMTDGSDLLHIAERHGHEELKEVFEDFLSQNTGRNSKQENDYEEDVI
  SFSTYSPSMPSPASLHELRKTHRRNTDRSEEPERSVEMKEEEAGAEARRSLSEGERESSENQYDDLYVFI
  PGFDTEGNSEEPLPHCRPPLLPPRPGTAASQLERPHFTSQGKVLEDQMERSQNWNDLNARPETREESSRE
  EKKEEAQEEEEEEENPYAFAETEDNEYDLILASKSVKKRTGNRSFIINRPPAPTPRPTHIPPKEETTPYI
  AQVFQQKAARRQSDGDKFYSLPKKPDKTRMEGPTFPSTRDYLTTGQEELILLQERVKNGKMSVDEALEKF
  KHWQMGKSGLEMIQQEKLRQLRDNIIGKRPEDENAYDKLTIVHHPSGNTAHNENMLYNSPFNSKFPARIQ
  VEKEFGFCCKKDH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033350
• ORF Size: 2349 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033350.2
• RefSeq Size: 3248 bp
• RefSeq ORF: 2352 bp
• Locus ID: 242248
• Cytogenetics: 3 63.04 cM