Pnpt1 (BC055826) Mouse Tagged ORF Clone

CAT#: MG210634

Pnpt1 (GFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pnpt1
• Synonyms: Old35, PNPase, RP23-313I4.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210634 representing BC055826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTGCAGGCTGTGCTGCTTGTGCCCGTGCCTCCGACCGCTGGGCTGCGGCCCCCTCGGCCGGC
  CCGGGCGGAATCGGGCACTCAGCTATTTGCAGATGCGAGCGTTGTGGAGCAGCACCGGATCCAGAGCGGT
  GACTGTAGACCTGGGACACAGAAAATTAGAAATATCCTCTGGGAAACTGGCAAGATTTGCTGATGGCTGT
  GCTGTGATACAGTCAGGCGATACTGCAGTAATGGTCACGGCAGTCAGCAAAACAAAAGCTTCGCCTTCCC
  AATTCATGCCGTTGGTGGTGGACTACCGACAGAAGGCTGCTGCAGCAGGCAGAATCCCCACAAACTACCT
  TAGAAGGGAGATTGGCTCCTCTGACAGAGAGGTTCTTACAAGTCGAGTAATAGATCGTTCAATTCGCCCT
  CTCTTTCCAGCCGGCTATTTTTATGATACTCAGGTTCTCTGTAATCTGCTAGCAGTAGATGGCATCAATG
  AACCTGATATCCTAGCAGTTAATGGTGCTTCTGTAGCCCTCTCCTTATCAGATATCCCTTGGAATGGACC
  TGTTGGGGCAGTACGAATAGGAATGATTGATGGAGAATGTGTCGTTAACCCAACAAGGAGAGAAATGTCT
  TCTAGCACTTTAAATTTAGTAGTTGCCGGAGCACCTAAAAGCCAAATTGTTATGTTGGAAGCCTCTGCAG
  AAAATATTCTACAGCAGGACTTTTGCCATGCTATCAAAGTTGGGGTGAAGTATACACAGCAGATAATTCA
  AGGCATCCAGCAGTTGGTAAAAGAAATCGGTGTTGCCAAGAGGACACCGCAGAAGATATTTACTCCTTCT
  GCAGAGATTGTGAAGTACACGAAGATAATTGCCATGGAGAAACTCTATGCGGTTTTTACAGATTATGAAC
  ATGATAAAGTTTCCAGGGATGAAGCTGTTAACAAGATAAGATTAGATACAGAGGAGCATCTAAAGGAAAA
  ATTTCCAGAGGTTGACCAATTTGAAATAATAGAATCCTTCAACATTGTTGCAAAGGAGGTTTTCCGAAGT
  ATTATTTTGAATGAATACAAAAGGTGTGATGGAAGAGATCTGACTTCACTTAGGAATATAAGTTGTGAGG
  TCGATATGTTTAAAACACTTCATGGATCAGCATTATTTCAGAGAGGACAAACACAGGTACTCTGTACTGT
  TACATTTGATTCATTAGAATCCAGTATTAAGTCAGATCAAATTATAACAGCTATAAATGGGGTAAAAGAT
  AAAAATTTCATGCTGCACTATGAGTTCCCTCCTTATGCAACCAATGAAACTGGCAAAGTTACTGGTGTGA
  ATCGAAGAGAACTTGGACATGGTGCTCTTGCCGAGAAAGCTTTGTGTCCTGTTATTCCCAAAGATTTTCC
  TTTTACCATAAGAGTTACATCTGAAGTCCTCGAATCAAATGGGTCATCTTCTATGGCATCTGCATGTGGT
  GGAAGTTTGGCATTAATGGATGCAGGGGTCCCAATTTCATCTGCTGTTGCAGGTGTAGCAGTGGGATTGG
  TTACCAAAACCAATCCAGAGAAAGGTGAAATAGAAGACTACCGTTTGCTAACAGATATTCTGGGAATTGA
  AGATTATAATGGTGACATGGATTTCAAAATAGCCGGTACAAATAAAGGAATAACTGCATTACAGGCTGAT
  ATTAAGTTACCTGGAGTACCAATTAAAATTATAATGGAAGCCATCCAACAAGCGTCAGTGGCAAAGAAGG
  AGATACTGCAGATAATGAACAAAACGATTTCAAAACCTCGAGCATCAAGAAAAGAAAATGGACCAGTTGT
  AGAAACAGTAAAGGTTCCATTATCAAAACGAGCAAAATTCGTTGGGCCTGGTGGATATCACTTAAAAAAA
  CTCCAGGCTGAGACAGGTGTAACAATTAGTCAGGTTGATGAAGAAACCTTCTCCATATTTGCACCAACAC
  CTACTGCAATGCATGAAGCAAGAGATTTCATTACAGAAATTTGCAGAGATGATCAAGAGCAACAATTAGA
  ATTTGGAGCAGTTTATACCGCGACAATAACTGAAATCAGAGACACTGGAGTGATGGTAAAACTGTATCCA
  AACATGACTGCAGTGCTGCTTCATAATTCACAACTTGACCAACGAAAGATTAAACATCCCACTGCCCTAG
  GACTAGAGGTTGGCCAAGAAATTCAGGTCAAATACTTTGGCCGTGATCCAGCTGATGGAAGAATGAGGCT
  TTCTCGTAAAGTACTTCAGTCTCCAGCTACAACTGCTCTCAAAACTCTAAATGATAGAAGCAGCATTGTA
  ATGGGAGAGCCTGTCTCACAGTCATCTAACTCTAACCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210634 representing BC055826
  Red=Cloning site Green=Tags(s)
  
  MAACRLCCLCPCLRPLGCGPLGRPGRNRALSYLQMRALWSSTGSRAVTVDLGHRKLEISSGKLARFADGC
  AVIQSGDTAVMVTAVSKTKASPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGSSDREVLTSRVIDRSIRP
  LFPAGYFYDTQVLCNLLAVDGINEPDILAVNGASVALSLSDIPWNGPVGAVRIGMIDGECVVNPTRREMS
  SSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKEIGVAKRTPQKIFTPS
  AEIVKYTKIIAMEKLYAVFTDYEHDKVSRDEAVNKIRLDTEEHLKEKFPEVDQFEIIESFNIVAKEVFRS
  IILNEYKRCDGRDLTSLRNISCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESSIKSDQIITAINGVKD
  KNFMLHYEFPPYATNETGKVTGVNRRELGHGALAEKALCPVIPKDFPFTIRVTSEVLESNGSSSMASACG
  GSLALMDAGVPISSAVAGVAVGLVTKTNPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQAD
  IKLPGVPIKIIMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVKVPLSKRAKFVGPGGYHLKK
  LQAETGVTISQVDEETFSIFAPTPTAMHEARDFITEICRDDQEQQLEFGAVYTATITEIRDTGVMVKLYP
  NMTAVLLHNSQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTALKTLNDRSSIV
  MGEPVSQSSNSNP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC055826
• ORF Size: 2351 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC055826, AAH55826
• RefSeq Size: 2598
• RefSeq ORF: 2351
• Locus ID: 71701