Pfkp (NM_019703) Mouse Tagged ORF Clone

CAT#: MG210641

Pfkp (GFP-tagged) - Mouse phosphofructokinase, platelet (Pfkp)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pfkp
• Synonyms: 1200015H23Rik; 9330125N24Rik; ATP-PFK; PFK-C; PFK-P
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210641 representing NM_019703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACTTGGATTCTTCGAGCTCCAGCGCCTATCCGAAGTACCTGGAGCACCTCTCTGGGGATGGCA
  AAGCTATCGGTGTCCTGACCAGCGGCGGGGATGCCCAAGGCATGAATGCTGCTGTCCGCGCTGTGGTGCG
  CATGGGAATCTACACGGGGGCCAAAGTGTACTTTATATATGAGGGTTACCAAGGCTTGGTGGATGGAGGC
  TCCAATATTGTGGAAGCCAAATGGGACTGTGTCTCCAGCATTCTGCAAGTGGGTGGGACCATCATCGGTA
  GTGCGCGCTGCAAAGCCTTTCGAAGCCGTGAAGGGCGCCTGAAAGCTGCCTGTAACTTGGCGCGCTTGGG
  CATAACCAATCTGTGCGTGATCGGCGGGGACGGAAGTCTCACGGGAGCCAACCTCTTCCGAAAGGAGTGG
  AGCGGACTTCTGGAAGAGCTGGCTCGGAATGGTGATATCGATAACGACACAGTGCAGAAGTATTCCTACC
  TCAACGTGGTGGGTATGGTGGGCTCCATTGACAATGACTTCTGTGGCACAGACATGACCATTGGTACAGA
  TTCAGCCCTGCACCGAATTATTGAAGTTGTTGATGCCATCATGACCACTGCTCAGAGCCACCAGAGGACC
  TTCGTCCTGGAGGTGATGGGGAGACACTGTGGTTACTTGGCCTTGGTGAGCGCCTTGACTTGCGGTGCCG
  ACTGGGTGTTCCTTCCGGAGTCTCCACCTGAGGAAGATTGGGAGGAAAATATGTGCCTCAAACTCTCAGA
  GAACCGAGCCCGAAAAAAGAGGCTGAATATCATCATTGTGTCTGAAGGAGCAATCGACATGCAAAATAAG
  CCAATCACCTCTGAGAAAATCAAGGAGCTTGTAGTGAAGAATTTGGGATTTGACACCCGGGTCACCATCC
  TTGGGCATGTCCAACGAGGAGGGACCCCATCTGCATTTGACAGGATTTTGGCCAGCCGTATGGGAGTGGA
  GGCTGTCATTGCCTTGCTGGAAGCTACCCCCGAGACCCCAGCCTGTGTCGTGTCACTAAGAGGAAACCAA
  GCAGTGCGCCTGCCACTGATGGAGTGTGTGCAAATGACCCAGGATGTACAGAAGGCAATGGATGAAAGAA
  GATTTAAGGAAGCCGTGAAACTCCGAGGAAGGCGTTTTGAGGGAAACCTGAACACCTATAAGCGTCTTGC
  CATTAAACTGCCTGATGAGAAGATTGTCAAGAGCAACTGCAATGTAGCCGTCATCAATGTAGGGGCACCT
  GCTGCCGGAATGAATGCAGCCGTGCGCTCTGCTGTTCGAGTCGGGATTGCAGACGGCCACAAGATGTTTG
  CAATCTATGACGGCTTTGAAGGATTCGCCAATGGCCAAATCAAAGAAATCGGCTGGGCAGATGTCGGAGG
  CTGGACTGGACAAGGAGGGTCCATTCTTGGGACAAAACGCACCCTACCTGGAAAGTACTTGGAGAAGATC
  GCAGAGCAGATGCATTCCCATAGTATCAATGCCCTTCTGATCATTGGAGGATTTGAGGCCTACCTAGGAC
  TCCTAGAGCTGGCAGCTGCCCGGGAGAAACATGAGGCGTTCTGTGTCCCCATGGTTATGGTTCCTGCTAC
  TGTCTCCAACAATGTGCCGGGTTCCGATTTCAGCATCGGGGCAGACACAGCTCTGAACACTATCACAGAC
  ACGTGCGACCGCATTAAACAGTCAGCCAGTGGGACCAAGCGCCGGGTGTTCATCATTGAGACCATGGGTG
  GATACTGTGGCTACCTGGCCAACATGGGGGCCCTTGCAGCAGGAGCTGATGCTGCCTACATCTTTGAAGA
  GCCATTTGATATCGGAGATTTGCAGTCCAACGTCGTGCACTTGACAGAGAAAATGAAGACCAGCATCCAG
  AGGGGCCTCGTACTCAGAAACGAAAGCTGCAGTGTAAATTATACCACTGACTTCATCTACCAGCTCTACT
  CAGAGGAGGGCAAAGGAGTGTTTGACTGCAGGAAAAACGTGCTGGGCCATATGCAGCAGGGGGGAGCACC
  GTCTCCATTCGATAGGAACTTTGGAACCAAGATTTCTGCCAAAGCAATGGAGTGGATCTCGGCCAAACTG
  AAGGGGTCCCAGGGCACAGGGAAAAAATTTGTTAGCGATGATTCCATTTGTGTCCTGGGAATTTGCAAGA
  GAGATCTCCTGTTTCAACCAGTGGCAGAGCTGAAGAAAGTCACTGACTTTGAGCACAGGATCCCCAAGGA
  GCAGTGGTGGCTGAAACTGCGGCCCATCATGAAGATCTTGGCCAAGTATGAGGCGAGCTATGACATGTCT
  GATTCAGGCAAGCTGGAGTCCTTGCAGCACCATGAAGAACTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210641 representing NM_019703
  Red=Cloning site Green=Tags(s)
  
  MSDLDSSSSSAYPKYLEHLSGDGKAIGVLTSGGDAQGMNAAVRAVVRMGIYTGAKVYFIYEGYQGLVDGG
  SNIVEAKWDCVSSILQVGGTIIGSARCKAFRSREGRLKAACNLARLGITNLCVIGGDGSLTGANLFRKEW
  SGLLEELARNGDIDNDTVQKYSYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRT
  FVLEVMGRHCGYLALVSALTCGADWVFLPESPPEEDWEENMCLKLSENRARKKRLNIIIVSEGAIDMQNK
  PITSEKIKELVVKNLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPETPACVVSLRGNQ
  AVRLPLMECVQMTQDVQKAMDERRFKEAVKLRGRRFEGNLNTYKRLAIKLPDEKIVKSNCNVAVINVGAP
  AAGMNAAVRSAVRVGIADGHKMFAIYDGFEGFANGQIKEIGWADVGGWTGQGGSILGTKRTLPGKYLEKI
  AEQMHSHSINALLIIGGFEAYLGLLELAAAREKHEAFCVPMVMVPATVSNNVPGSDFSIGADTALNTITD
  TCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEPFDIGDLQSNVVHLTEKMKTSIQ
  RGLVLRNESCSVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKL
  KGSQGTGKKFVSDDSICVLGICKRDLLFQPVAELKKVTDFEHRIPKEQWWLKLRPIMKILAKYEASYDMS
  DSGKLESLQHHEEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019703
• ORF Size: 2352 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019703.2, NP_062677.1
• RefSeq Size: 3986
• RefSeq ORF: 2355
• Locus ID: 56421
• Gene Summary: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProtKB/Swiss-Prot Function]