Rasa1 (NM_145452) Mouse Tagged ORF Clone

CAT#: MG210785

Rasa1 (GFP-tagged) - Mouse RAS p21 protein activator 1 (Rasa1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rasa1
• Synonyms: Gap; Rasa; RasGAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210785 representing NM_145452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGGCCGAGGCGGGCAGTGAGGAAGGCGGCCCTGCGACGGCGGGCCCCGGCGGCGGCGCGGCCA
  CGGGCTCCAGCGCCTACTCGGCAGCGTGCCGGGTCAAGTTACCCGCGGCCCTGCCGGTGGCAGCCGCCCC
  GTGTCCCGGGCTGGCGGACGCCGACTTGGTTGCGGCCCTCGGTGGCGGAGCCGCGTCGGGGTCCGGGTTC
  CTGGGGACCGGGCCGGTGGCCGGTGTCTTGGGGGGCGCCGCGCTGACCGGCAGTGCTGCTGCTGGCGTGG
  CTGGTGCTGCGGCCGCCGGACCTGCGGGAGACGTCGCTTTCACCAAGGGGACTCTGTCTTTGCCTGCTGA
  GACGCTCGGGCCGGGCGGCGGATTCCCTCCTCTGCCGCCGCCTCCTCAGCTGCCCCCTTTGGGCTCCGGT
  TTGGGGACCGTGGATGAAGGTGACTCCCTGGATGGACCAGAATACGAAGAGGAAGAGGTGGCCATACCGC
  TGACCGCACCTCCCACTAACCAGTGGTATCATGGAAAACTTGATAGAACTATAGCAGAAGAACGCCTCAG
  GCAGGCAGGGAAATCTGGAAGCTATCTTATAAGAGAGAGTGATCGGAGGCCGGGGTCCTTTGTACTTTCA
  TTCCTTAGTCAGACAAATGTTGTCAATCATTTTAGGATTATTGCTATGTGTGGTGATTACTACATTGGTG
  GAAGGCGTTTTTCTTCACTCTCAGACCTAATAGGTTATTACAGTCACGTTTCTTGTTTACTTAAAGGAGA
  AAAATTACTCTATCCAGTTGCACCACCAGAGCCAGTAGAAGATAGAAGGCGTGTACGAGCCATTCTGCCC
  TACACAAAAGTACCAGACACTGATGAAATAAGTTTTTTAAAAGGAGATATGTTCATTGTTCACAATGAGT
  TAGAAGATGGATGGATGTGGGTTACCAATCTAAGAACAGATGAGCAAGGCCTTATTGTTGAAGACCTAGT
  GGAAGAGGTGGGCCGAGAAGAAGATCCACACGAAGGCAAAATATGGTTCCACGGGAAAATTTCCAAACAA
  GAAGCTTATAATTTATTAATGACAGTTGGACAAGTCTGCAGCTTTCTTGTGAGGCCCTCAGATAATACTC
  CTGGAGATTATTCTCTGTATTTTCGGACCAATGAAAATATTCAGCGATTTAAAATTTGTCCAACACCTAA
  CAACCAGTTTATGATGGGAGGCCGCTATTATAACAGTATCGGGGACATCATAGACCATTATCGGAAAGAA
  CAGATTGTTGAAGGCTATTATCTTAAGGAACCTGTACCAATGCAGGATCAAGGGCAAGTACTGAACGACA
  CTGTGGATGGGAAGGAGATCTATAACACAATTCGTCGGAAGACAAAGGATGCTTTTTATAAAAATATTGT
  TAAAAAAGGTTATCTTCTGAAGAAGGGTAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAAGGC
  AGTGATGCTCAACTTATTTATTTTGAAAGTGAAAAACGAGCTACAAAACCAAAAGGGCTAATAGACCTTA
  GTGTGTGTTCTGTCTACGTTGTCCATGACAGTCTCTTCGGCAGGCCAAACTGTTTTCAGATAGTAGTTCA
  GCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATG
  AAAGGTCTGCAAGCATTTTGCAGTTTACGGAAAAGTAGCCCAGGGACATCTAATAAACGCCTTCGTCAGG
  TCAGCAGCCTTGTTTTACATATTGAAGAAGCCCATAAACTACCAGTAAAACACTTTACTAATCCATATTG
  TAACATCTACTTGAATAGCGTCCAAGTAGCAAAAACTCATGCAAGGGAGGGGCAAAACCCAGTATGGTCA
  GAGGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATCACACTTAGTAATAAAACAA
  AGAAAAGCAAAGATCCAGATATCTTATTTATGCGTTGCCAGTTGAGCAGGTTACAGAAAGGACATGCAAC
  AGATGAATGGTTTCTACTAAGTTCCCATATACCACTAAAAGGTATTGAACCAGGATCTTTGCGTGTTCGA
  GCACGATACTCCATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAA
  AGGAACTTCATGTCGTCTATGCTTTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCT
  ACTAAAGATATTTCTTCATGAAAAGCTTGAATCATTGTTGTTATGCACACTAAATGACAGAGAGATAAGT
  ATGGAAGATGAAGCCACTACCCTATTTCGAGCTACAACACTTGCAAGTACCTTGATGGAGCAGTATATGA
  AAGCTACTGCCACACAGTTTGTTCATCATGCTTTGAAAGACTCCATTTTAAAGATTATGGAAAGCAAGCA
  ATCATGTGAGTTAAGTCCGTCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAGCACACCTATTG
  AGCATACTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACACTGAGATATA
  TCTATGGGTGTTTACAGAAATCAGTTCAGCATAAGTGGCCTACAAATAACACCATGAGAACAAGAGTCGT
  TAGTGGTTTTGTTTTTCTTCGCCTTATCTGCCCTGCTATCCTGAATCCACGGATGTTTAATATCATCTCA
  GATTCCCCATCTCCTATTGCTGCAAGAACACTGACATTAGTGGCTAAATCTGTGCAGAATTTAGCAAATC
  TTGTGGAATTTGGAGCTAAGGAGCCTTATATGGAAGGTGTCAATCCATTTATAAAAAGTAATAAACATCG
  CATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACACTACAGAGCATTCTAGAACT
  GACCTGTCTCGTGACTTAGCAGCATTGCATGAGATTTGTGTAGCTCATTCAGATGAACTGAGAACACTCA
  GCAATGAGCGTGGTGTACAGCAGCACGTACTGAAAAAGCTTCTGGCGATAACAGAACTGCTTCAACAAAA
  ACAAAACCAGTATACAAAAACCAATGACATCAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210785 representing NM_145452
  Red=Cloning site Green=Tags(s)
  
  MMAAEAGSEEGGPATAGPGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLADADLVAALGGGAASGSGF
  LGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSG
  LGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLS
  FLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILP
  YTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQ
  EAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKE
  QIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEG
  SDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWM
  KGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWS
  EEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVR
  ARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREIS
  MEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLL
  SILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIIS
  DSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRT
  DLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDIR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145452
• ORF Size: 3114 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145452.3, NP_663427.2
• RefSeq Size: 5101
• RefSeq ORF: 3117
• Locus ID: 218397