Pml (BC020990) Mouse Tagged ORF Clone

CAT#: MG210910

Pml (GFP-tagged) - Mouse promyelocytic leukemia (cDNA clone MGC:13700 IMAGE:4188386)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pml
• Synonyms: Trim19
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210910 representing BC020990
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTGAACCAGTTTCCGTGCAGAAGGTACCTGCACCCCCTGGATCTCCCTGTCGACAACAGGACT
  CTGCCCTGACCCCCACGCCCACCATGCCTCCCCCAGAGGAACCCTCCGAAGACTATGAACACAGCCAAAG
  CCCTGCAGAGCAAGCCATACAGGAGGAATTTCAGTTTCTGCGCTGCCCGAGCTGCCAGGCCCAAGCCAAG
  TGCCCCAAACTGCTGCCTTGCCTGCACACGCTGTGCTCCGGATGCCTGGAGGCGCCTGGCCTGCAGTGCC
  CCATCTGCAAGGCTCCTGGGCAGGCCGATGCTAATGGGGAGGCCCTGGATAACGTGTTCTTCGAGAGCCT
  GCAGCGGCGCCTGGCGGTGTTCCGGCAGATCGTGGATGCTCAGGCTGCGTGCACCCGCTGCAAAGGCTTG
  GCCGACTTCTGGTGTTTCGAGTGTGAACAGCTCATTTGCAGCAAGTGCTTCGAAGCACACCAGTGGTACC
  TCAAGCATGAGGCCCGGCCCCTGGCCGATCTCCGCGACAATTCAGTGAGCAGCTTCCTCGACAGTACGCG
  CAAGTCCAATATCTTCTGCTCCAATACCAACCACCGCAACCCTGCGCTGACTGACATCTACTGCCGAGGC
  TGCGCCAAGCCTCTGTGTTGCACATGCGCGCTCCTGGACCGCAACCACAGCCATCTCCATTGCGATATTG
  GTGAGGAGATTCAGCAGTGGCATGAGGAACTAGGCACCATGACACAGACTCTGGAGGAGCAGGGCAGAAC
  CTTCGACAGTGCCCATGCACAGATGTGCTCAGCTATAGGACAGCTGGACCACGCACGCGCAGACATTGAG
  AAGCAGATCCGCGCACGCGTGCGCCAGGTGGTAGACTACGTGCAGGCTCAGGAGCGCGAGCTGCTCGAGG
  CGGTGAATGACCGCTACCAGCGCGACTACCAGGAAATAGCTGGCCAGCTGAGCTGCCTGGAAGCTGTGCT
  GCAGCGCATCCGCACTAGTGGGGCGCTGGTCAAGAGGATGAAGCTCTATGCCTCCGACCAGGAGGTGCTG
  GATATGCACAGCTTTCTGCGCAAGGCACTCTGTAGCTTGCGCCAGGAGGAGCCCCAGAACCAGAAAGTCC
  AGCTGCTCACCAGAGGTTTCGAGGAGTTCAAGCTGTGCCTGCAGGACTTCATCTCCTGCATCACCCAGAG
  GATAAATGCAGCTGTAGCCAGCCCAGAGGCAGCCAGCAATCAACCAGAGGCAGCCAGCACTCACCCAGTG
  ACAACCAGCACGCCTGAGGACCTTGTGCAGGAGGCTTCTCAGACAGTCGGCTCCATGAAGAGGAAGTGCT
  CCCACGAAGATTGCTCCAGGAAGATCATCAAGATGGAGTCCACAGAGGAGAACGAGGACAGGTTGGCCAC
  AAGCTCCCCCGAGCAGTCCTGGCCCAGCACTTTCAAGGCCACCTCCCCTCCCCATCTGGATGGGACTTCC
  AACCCTGAGAGCACCGTCCCTGAAAAAAAGATCCTCCTGCCCAACAACAATCATGTTACCAGTGACACAG
  GGGAAACAGAGGAGCGAGTTGTGGTGATCAGCAGCTCAGAGGATTCGGACACCGAGAATCTGTCCTCCCA
  CGAGTTGGACGATAGCAGCAGTGAGTCCAGCAGCCTGCAGCTGGAGGGCCCCAATTCCCTCAAGGCCTTG
  GATGAGAGCCTCGCCGAACCCCACTTAGAAGACAGGACCCTGGTTTTCTTTGACCTCAAGATTGACAATG
  AAACCCAGAAAATTAGCCAGCTGGCCGCGGTGAACCGGGAAAGCAAGTTCCGTGTGCTCATCCAGCCCGA
  GGCCTTCAGTGTCTACTCCAAAGCTGTCTCCCTGGAGGCAGGGCTCCGGCACTTCCTCAGCTTCCTCACC
  ACCATGCATCGTCCCATCTTGGCGTGTTCTAGGCTGTGGGGGCCGGGACTCCCCATCTTCTTCCAGACCC
  TGAGTGATATTAACAAGCTGTGGGAATTCCAGGACACCATCTCAGGTTTCTTGGCCGTGCTGCCCCTCAT
  CCGGGAACGCATACCCGGCGCTAGCAGCTTCAAACTTGGGAACCTAGCCAAGACCTACCTGGCGAGAAAC
  ATGAGCGAACGCAGCGCTCTGGCTTCTGTGCTGGCCATGAGGGACTTGTGCTGCCTCCTTGAGATCTCCC
  CAGGACTGCCGCTGGCCCAGCATATCTACTCCTTTAGTAGCTTGCAGTGTTTTGCTTCCCTGCAGCCCCT
  AATTCAGGCCAGCGTCCTGCCACAGTCCGAGGCCCGCCTCTTGGCCCTCCACAATGTGAGCTTTGTAGAG
  TTGCTGAATGCATATCGCACCAACAGGCAAGAAGGCTTGAAGAAGTATGTCCACTATCTGAGCCTGCAGA
  CCACCCCGTTGTCATCGTCGGCTTCCACCCAAGTTGCCCAATTCCTGCAGGCTCTTAGCACCCACATGGA
  AGGACTGTTGGAAGGCCATGCCCCTGCTGGGGCAGAAGGCAAAGCTGAAAGTAAGGGATGCCTGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210910 representing BC020990
  Red=Cloning site Green=Tags(s)
  
  METEPVSVQKVPAPPGSPCRQQDSALTPTPTMPPPEEPSEDYEHSQSPAEQAIQEEFQFLRCPSCQAQAK
  CPKLLPCLHTLCSGCLEAPGLQCPICKAPGQADANGEALDNVFFESLQRRLAVFRQIVDAQAACTRCKGL
  ADFWCFECEQLICSKCFEAHQWYLKHEARPLADLRDNSVSSFLDSTRKSNIFCSNTNHRNPALTDIYCRG
  CAKPLCCTCALLDRNHSHLHCDIGEEIQQWHEELGTMTQTLEEQGRTFDSAHAQMCSAIGQLDHARADIE
  KQIRARVRQVVDYVQAQERELLEAVNDRYQRDYQEIAGQLSCLEAVLQRIRTSGALVKRMKLYASDQEVL
  DMHSFLRKALCSLRQEEPQNQKVQLLTRGFEEFKLCLQDFISCITQRINAAVASPEAASNQPEAASTHPV
  TTSTPEDLVQEASQTVGSMKRKCSHEDCSRKIIKMESTEENEDRLATSSPEQSWPSTFKATSPPHLDGTS
  NPESTVPEKKILLPNNNHVTSDTGETEERVVVISSSEDSDTENLSSHELDDSSSESSSLQLEGPNSLKAL
  DESLAEPHLEDRTLVFFDLKIDNETQKISQLAAVNRESKFRVLIQPEAFSVYSKAVSLEAGLRHFLSFLT
  TMHRPILACSRLWGPGLPIFFQTLSDINKLWEFQDTISGFLAVLPLIRERIPGASSFKLGNLAKTYLARN
  MSERSALASVLAMRDLCCLLEISPGLPLAQHIYSFSSLQCFASLQPLIQASVLPQSEARLLALHNVSFVE
  LLNAYRTNRQEGLKKYVHYLSLQTTPLSSSASTQVAQFLQALSTHMEGLLEGHAPAGAEGKAESKGCLA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC020990
• ORF Size: 2519 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC020990, AAH20990
• RefSeq Size: 4296
• RefSeq ORF: 2519
• Locus ID: 18854