Sh3rf1 (BC060113) Mouse Tagged ORF Clone

CAT#: MG210987

Sh3rf1 (GFP-tagged) - Mouse SH3 domain containing ring finger 1 (cDNA clone MGC:63290 IMAGE:6405370)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sh3rf1
• Synonyms: 2200003J05Rik|Posh|R75531|Sh3md2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210987 representing BC060113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGTCTGCCTTGTTGGACCTTCTGGAGTGCCCTGTGTGTCTAGAACGCCTGGATGCTTCCGCAA
  AGGTCTTACCCTGCCAGCATACCTTTTGCAAACGCTGTTTGCTGGGGATTGTGGGTTCCCGGAATGAACT
  CAGATGTCCCGAATGCCGGACTCTTGTTGGCTCTGGGGTCGACGAGCTCCCCAGTAACATCCTACTGGTC
  AGACTTCTGGATGGCATCAAGCAGAGGCCTTGGAAACCCGGCCCTGGTGGGGGCGGCGGGACCACCTGCA
  CAAACACATTAAGGGCGCAGGGCAGCACTGTGGTTAATTGTGGCTCGAAAGATCTGCAGAGCTCCCAGTG
  TGGACAGCAGCCTCGGGTGCAAGCCTGGAGCCCCCCAGTGAGGGGAATACCTCAGTTACCGTGTGCCAAA
  GCATTATATAACTACGAAGGAAAAGAGCCCGGAGACCTTAAGTTCAGCAAAGGCGACATCATCAAACCTT
  TACCTCAGCCCCCGCCTCAGTGCAAAGCACTTTACGACTTTGAAGTGAAAGACAAGGAAGCTGACAAAGA
  TTGCCTTCCCTTCGCAAAGGACGACGTACTGACCGTGATCCGCAGAGTGGATGAAAACTGGGCTGAAGGA
  ATGCTGGCAGATAAAATAGGAATATTTCCAATTTCATACGTGGAGTTTAACTCAGCTGCCAAGCAGCTGA
  TAGAGTGGGATAAGCCTCCCGTGCCAGGAGTGGACACGGCAGAATGCCCCTCAGCGACGGCGCAGAGCAC
  CTCTGCCTCAAAGCACCCCGACACCAAGAAGAACACCAGGAAGCGACACTCCTTCACCTCCCTCACCATG
  GCCAACAAGTCTTCCCAGGGGTCCCAGAACCGCCACTCCATGGAGATCAGCCCTCCTGTGCTCATCAGTT
  CCAGCAACCCCACAGCCGCAGCCCGCATCAGCGAACTGTCCGGGCTCTCCTGCAGCGCCCCGTCTCAGGT
  CCATATAAGCACCACTGGGTTAATTGTGACCCCACCCCCTAGCAGCCCGGTGACAACTGGCCCTGCGTTC
  ACGTTCCCTTCAGATGTCCCCTACCAAGCTGCCCTTGGAAGTATGAATCCTCCACTTCCCCCACCCCCTC
  TCCTGGCGGCCACCGTACTCGCCTCCACCCCGTCAGGCGCTACTGCTGCTGTTGCTGCTGCTGCTGCCGC
  CGCCGCTGCTGGAATGGGACCCAGGCCTGTGATGGGGTCCTCTGAACAGATTGCACATTTACGGCCTCAG
  ACTCGTCCCAGTGTATATGTTGCTATATATCCGTACACTCCCCGGAAGGAAGACGAACTGGAGCTGAGGA
  AAGGGGAGATGTTTTTGGTGTTTGAGCGTTGCCAGGACGGCTGGTACAAAGGGACATCGATGCATACCAG
  CAAGATAGGCGTTTTCCCTGGCAACTATGTGGCGCCCGTCACAAGGGCGGTGACGAATGCCTCCCAAGCT
  AAAGTCTCTATGTCTACTGCGGGTCAAGCAAGTCGCGGGGTGACCATGGTCAGCCCTTCCACTGCAGGAG
  GACCTACACAGAAGCCCCAAGGAAACGGCGTGGCCGGAAATCCCAGCGTCGTCCCCACGGCTGTGGTGTC
  AGCAGCTCATATCCAGACAAGTCCTCAGGCTAAGGTCCTGCTGCACATGTCTGGGCAGATGACAGTCAAT
  CAGGCCCGCAATGCTGTGAGGACAGTTGCAGCACATAGCCAGGAACGCCCCACGGCAGCAGTGACTCCCA
  TCCAGGTCCAGAATGCCGCCTGCCTCGGTCCTGCATCCGTGGGCCTGCCCCATCATTCTCTGGCCTCCCA
  ACCTCTGCCTCCAATGGCGGGTCCTGCTGCCCACGGTGCTGCCGTCAGCATCAGTCGAACCAATGCCCCC
  ATGGCCTGCGCTGCAGGGGCTTCTCTGGCCTCCCCAAATATGACCAGTGCCGTGTTGGAGACAGAGCCCA
  GTGGTCGCACAGTGACCATCCTCCCTGGACTCCCCACATCTCCAGAGAGTGCTGCATCAGCGTGTGGGAA
  CAGTTCAGCTGGGAAACCAGACAAGGACAGTAAGAAAGAAAAAAAGGGCCTACTGAAGCTGCTTTCTGGT
  GCCTCCACCAAACGCAAGCCCCGAGTCTCCCCTCCAGCATCACCTACCCTGGATGTGGAGCTGGGTGCTG
  GGGAGGCTCCCTTGCAGGGAGCAGTAGGTCCTGAGCTGCCGCTAGGGGGCAGCCACGGCAGAGTGGGGTC
  ATGCCCCACAGATGGTGATGGTCCAGTGGCCGCTGGAACAGCAGCCCTAGCCCAGGATGCCTTCCACCGC
  AAGACAAGCTCCCTGGACTCCGCAGTGCCCATTGCTCCACCACCTCGCCAGGCCTGCTCCTCCCTGGGCC
  CAGTCATGAATGAGGCCCGGCCTGTTGTTTGTGAAAGGCACAGGGTGGTGGTTTCCTACCCTCCTCAGAG
  TGAGGCCGAACTTGAACTCAAGGAAGGAGATATTGTGTTTGTTCATAAGAAACGAGAGGACGGCTGGTTC
  AAAGGCACGTTACAGAGGAATGGGAAGACTGGCCTTTTCCCAGGGAGCTTTGTGGAAAACATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210987 representing BC060113
  Red=Cloning site Green=Tags(s)
  
  MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLV
  RLLDGIKQRPWKPGPGGGGGTTCTNTLRAQGSTVVNCGSKDLQSSQCGQQPRVQAWSPPVRGIPQLPCAK
  ALYNYEGKEPGDLKFSKGDIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEG
  MLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAQSTSASKHPDTKKNTRKRHSFTSLTM
  ANKSSQGSQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPAF
  TFPSDVPYQAALGSMNPPLPPPPLLAATVLASTPSGATAAVAAAAAAAAAGMGPRPVMGSSEQIAHLRPQ
  TRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQA
  KVSMSTAGQASRGVTMVSPSTAGGPTQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMSGQMTVN
  QARNAVRTVAAHSQERPTAAVTPIQVQNAACLGPASVGLPHHSLASQPLPPMAGPAAHGAAVSISRTNAP
  MACAAGASLASPNMTSAVLETEPSGRTVTILPGLPTSPESAASACGNSSAGKPDKDSKKEKKGLLKLLSG
  ASTKRKPRVSPPASPTLDVELGAGEAPLQGAVGPELPLGGSHGRVGSCPTDGDGPVAAGTAALAQDAFHR
  KTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWF
  KGTLQRNGKTGLFPGSFVENI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060113
• ORF Size: 2585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC060113, AAH60113
• RefSeq Size: 5074
• RefSeq ORF: 2585
• Locus ID: 59009