Snap91 (BC031773) Mouse Tagged ORF Clone

CAT#: MG211010

Snap91 (GFP-tagged) - Mouse synaptosomal-associated protein 91 (cDNA clone MGC:25399 IMAGE:4511467)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snap91
• Synonyms: AP180, F1-20, 91kDa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211010 representing BC031773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCCAAACGCTCACGGATCGGATCGCCGCCGCTCAGTACAGCGTGACTGGCTCTGCTGTAGCAA
  GAGCAGTCTGCAAAGCCACCACTCATGAGGTGATGGGCCCCAAGAAGAAGCACCTGGACTATTTGATCCA
  GGCTACCAATGAGACCAATGTCAATATCCCTCAGATGGCCGACACCCTCTTTGAGCGGGCGACAAACAGT
  AGCTGGGTGGTGGTATTTAAAGCTTTAGTGACCACACACCATCTCATGGTGCATGGAAATGAGAGATTTA
  TTCAGTATTTGGCCTCTAGGAATACGCTATTCAATCTCAGCAACTTTCTAGATAAAAGTGGATCCCACGG
  TTATGATATGTCTACGTTCATACGGCGTTACAGTAGATACTTGAATGAAAAGGCTTTCTCCTACAGACAG
  ATGGCATTTGACTTTGCCAGAGTGAAGAAAGGGGCTGACGGTGTCATGAGGACGATGGTTCCTGAAAAGC
  TCCTGAAGAGTATGCCAATCCTGCAGGGGCAGATCGATGCACTGCTGGAGTTTGATGTGCATCCAAATGA
  ACTAACCAATGGTGTCATAAATGCTGCATTTATGCTTCTTTTCAAAGATCTTATCAAACTGTTTGCTTGC
  TACAATGACGGCGTCATTAACTTACTTGAAAAATTTTTCGAGATGAAGAAAGGTCAATGCAAAGACGCGC
  TAGAAATTTACAAGCGATTTCTAACTAGAATGACGAGGGTGTCTGAATTCCTCAAGGTCGCCGAGCAAGT
  TGGTATTGATAAAGGTGACATTCCCGACCTCACACAGGCTCCCAGCAGTCTTATGGAGACCCTTGAACAA
  CATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAATGAAGGATCTGGTGCTCCCTCTCCACTAAGTA
  AGTCTTCTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAACTATCGACACATCCCCGCC
  AGTTGACATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAGCCATCAAGCGATCTCCTT
  GATCTTCAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAGTGCCTCCTTCTGGGGGTG
  CGACCGCCTGGGGAGACCTTTTGGGAGAGGATTCCTTGGCTGCACTTTCCTCTGTTCCCTGTGAAGCACC
  GATTTCAGACCCATTTGCACCAGAGCCTTCCCCTCCTACTACAACCACTGAGCCTGCTTCAGCCTCTGCC
  TCGACCACCACAGCTGTGACGGCTGTCACTACGGAAGTGGATCTCTTTGGAGATGCCTTTGCAGCTTCTC
  CTGGGGAGGCCCCTGCAGCATCCGAAGGGGCTACCGCACCAGCTACCCCGGCCCCAGTGGCTGCAGCTCT
  TGATGCATGCTCAGGAAATGACCCTTTTGCCCCATCTGAAGGTAGCGCAGAGGCTGCACCTGAGCTGGAC
  CTCTTTGCAATGAAGCCACCTGAGACCAGCGCTCCTGTAGTTACCCCTACAGCTAGCACAGCCCCTCCAG
  TTCCCGCAACTGCTCCTTCTCCTGCTCCCACGGCTGTGGCAGCCACTGCTGCCACCACCACCGCCGCCGC
  TGCAGCTACCACCACTGCCACCACCTCTGCTGCTGCTGCCACCACCGCCGCTGCTCCTCCTGCTCTAGAT
  ATCTTTGGTGATTTGTTTGATTCTGCTCCTGAAGTTGCTGCAGCACCTAAGCCAGACGCGGCTCCTAGCA
  TAGACCTGTTTGGCACAGATGCTTTCTCCTCCCCGCCACGAGGGGCCTCTCCGGTGCCTGAGAGTTCTCT
  CACTGCTGACCTCTTATCTGTGGACGCATTTGCAGCGCCGTCTCCTGCATCCACTGCCTCTCCTGCAAAG
  GCGGAGTCCTCGGGTGTCATAGACCTTTTTGGGGATGCGTTTGGAAGTGGTGCTTCTGAAACCCAGCCAG
  CACCACAGGCTGTTTCTAGTTCATCAGCATCGGCAGATCTACTAGCTGGATTTGGGGGTTCTTTCATGGC
  CCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCTGCTGCAACCCAGTTTCGAGGCAGCTTTTGGA
  ACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCATCAGGCGATCTTCTGATGCCAACCATGGCAC
  CATCCGGGCAGCCTGCCCCTGTCTCAATGGTCCCACCCAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGG
  AAGTGACCTTGACTCGTCTCTGGCCAGTTTAGTAGGCAATCTTGGGATTTCTGGTACCACATCAAAAAAG
  GGAGATCTCCAGTGGAATGCTGGGGAGAAAAAGCTGACTGGTGGAGCCAACTGGCAACCGAAAGTCACTC
  CAGCCACATGGTCAGCGGGTGTTCCCCCGCCTCCTTCAGGGACAGGCATGACCATGATGTCTCAGCAGCC
  AGTCATGTTTGCACAGCCCATGATGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCGGCACACAGCTTTCT
  CCAAGCCCTACACCTGCCACTCAGAGTCCCAAGAAACCTCCAGCCAAGGACCCGTTAGCGGATCTTAACA
  TCAAGGATTTCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211010 representing BC031773
  Red=Cloning site Green=Tags(s)
  
  MSGQTLTDRIAAAQYSVTGSAVARAVCKATTHEVMGPKKKHLDYLIQATNETNVNIPQMADTLFERATNS
  SWVVVFKALVTTHHLMVHGNERFIQYLASRNTLFNLSNFLDKSGSHGYDMSTFIRRYSRYLNEKAFSYRQ
  MAFDFARVKKGADGVMRTMVPEKLLKSMPILQGQIDALLEFDVHPNELTNGVINAAFMLLFKDLIKLFAC
  YNDGVINLLEKFFEMKKGQCKDALEIYKRFLTRMTRVSEFLKVAEQVGIDKGDIPDLTQAPSSLMETLEQ
  HLNTLEGKKPGNNEGSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDIFATASAAAPVSSAKPSSDLL
  DLQPDFSGAAAGAAAPVVPPSGGATAWGDLLGEDSLAALSSVPCEAPISDPFAPEPSPPTTTTEPASASA
  STTTAVTAVTTEVDLFGDAFAASPGEAPAASEGATAPATPAPVAAALDACSGNDPFAPSEGSAEAAPELD
  LFAMKPPETSAPVVTPTASTAPPVPATAPSPAPTAVAATAATTTAAAAATTTATTSAAAATTAAAPPALD
  IFGDLFDSAPEVAAAPKPDAAPSIDLFGTDAFSSPPRGASPVPESSLTADLLSVDAFAAPSPASTASPAK
  AESSGVIDLFGDAFGSGASETQPAPQAVSSSSASADLLAGFGGSFMAPSTTPVTPAQNNLLQPSFEAAFG
  TTPSTSSSSSFDPSGDLLMPTMAPSGQPAPVSMVPPSPAMAASKGLGSDLDSSLASLVGNLGISGTTSKK
  GDLQWNAGEKKLTGGANWQPKVTPATWSAGVPPPPSGTGMTMMSQQPVMFAQPMMRPPFGAAAVPGTQLS
  PSPTPATQSPKKPPAKDPLADLNIKDFL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031773
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC031773.1
• RefSeq Size: 3333
• RefSeq ORF: 2606
• Locus ID: 20616
• Gene Summary: Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats. [UniProtKB/Swiss-Prot Function]