Gprasp1 (BC027187) Mouse Tagged ORF Clone

CAT#: MG211032

Gprasp1 (GFP-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (cDNA clone MGC:25275 IMAGE:4505972)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gprasp1
• Synonyms: GASP1, GASP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211032 representing BC027187
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAGGATGAGGTCGTTATTGGGT
  CCTGGTTCTGGGCAGATGATGAAGAGATCAACTTACAGGCTGATGACGAGTCTATTTTTGGATCTTGGTT
  CTGGGGCACTGGTGAGAACAGCCTTAGATCTGTTGGAGTCAATTGTGAGAAGATGCCGAAGGCTGGAGAA
  AAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGATGTCAATACAGAGGCTGAGGTTGAAGAACAGGCCA
  GGTCGGCATCTACAAAAGCAACAATCTTTGTGCCTTGGTTTTGGTCTGAAAAGCAGCCGAACATGGATTT
  AGGGTCTGAACCTTGCTCAGATATCATGGCAGGAGCTGAGGAGGAGCCCATAATTGGGCCCTGGTTCTGG
  GCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAACAGTAAGTCTAGCCTTGAGGATGAGGAAGAACCCA
  TTAGATCCCCTTGGTTTGGGGCCAGAGAACAAACCGATATGAAGTATGCAGCTGGTATCAGATACAAGCC
  TATGGCAGAAGCTGAGGATGCTAACAAAAAGTCTTGTGTCTGGGCAAAAGAACCCTGTTTGTATCCTACC
  AATAGAGAATGCTTGAAATCTACTCTGGGAGAAAAAGAAGACACTGTTGATCCATGGCTCTGGTCCAATA
  ATTATCCAAGGACCAAGACCATCACAGGGTCCTGGCTATGGGCTGCAGAAGAGGGAAATATAGATGATGA
  GACTGGAGAAAAGATTAAACTACCAACTTTAGAGGACAATGCATTCAATTCTTGGTTCTGGAAAGAAAAT
  GAAGAAAGCATTGTAGAGGCTCCCAAGAGAGAAGAATTCAGGCCAGAAGCGGAAGAGGAAGACATTATTG
  GTTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTGAGCCAGCTGCTAAGATTAATGAAGAGAACAAGAT
  AGCATCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAATGAAGAGGCCAGTCTAGAGGCAGTG
  AGAAGGGGTACTTTTGAGTCTGCTCCTGGGATTAAGGAAGAGAAAGTTACTGGGTCATGGTTCTGGACTG
  ATAAGGCCAAAGTAGGGGCTGGGTCCCAGACTGTCGAAACTGGGTCAGAAACTGAAGAGGAAGCAATTTT
  TGAGTCCTTGATCTGGGCTGCAAAAAAGGACAGTATTCAAGCAGGAGTAAAGCGTGTGTCCAAGCCAAAA
  GATGACGGTAACATAGCTGTTGGGTCCTGGCTCTGGTCTAGTGACAAGGCCACAAAAGAAGCTAAAACTC
  TGATTGTCAGTGAGGCCAGTCCAGAAAATGGGAAAGAGTCAGTTGTTAAGTTTGGGTCAAGAGCAAAAGA
  TGAGGTGATTAACAAGACTGGCAGTGGTGACAATTGTAAACATAGTACAGAAGCTGAAACCATAGTGGGA
  GCCTGGTTCTGGGAAGGAGATGAAGCTAGCTTTGAATCAAATCCTGTACCTGTATGCAAGGCCGTTTGTG
  AACCCGAGTCTTCAGCTGAGCACGAACCTGACCCTTCCCGCAGGCCTCAGAGCTGGGATGAGGTCACTGT
  TCAGTTTAAGGCTGGTCCGTGGGGAAAAGCTGGCTTCCCACCCATGAACCCCTTCAGATTCCCCAAAGAA
  GCGGCATCTCTGTTTGCAGAAATGTTTGGGGGAAAGCCTAAGCTTGTGGAAGTTGGTCCAGAAAGGGAGC
  CTGAACCCCAGTTCCCATTTCAGTATGATCCCTCTTACCGCTCAGTCCGAGAAATTCGCGAGCATCTTAA
  GGCCAGGGAAAGTGCACAGCCAGAGAATTGGTCTTGCAACTGCATCCAGTGTGAGCTTAGAATTGGTTCT
  GAGGAGTTTGAGGAGCTTCTTTTACTGATGGACAGAAATCGTGATCCTTTTATCCATGAGATATCTAAGA
  TTGCAATGGGCATGCGAGGTGCTTCTCAGTTTACCCGGGATTTCATTCGAAACTCGGGCGTTGTCTCCCT
  GATTGAAGCCTTACTTAACTATCCGTCCTCCCGAGTGAGGACAAGGTTTTTGGAAAATATGGTCCGCATG
  GCTCCCCCTTATCCAGACCTGAACATGATTGAGACATACGTGTGTCAGATTTGCGAGGACACCTTTGACT
  ATGACTTGGATTCCCCTGATCAGTTGTCCGGATTGACAATGATTACACACCTCACTGCTACTTCTGACTA
  TCACAAGGTGGTTGTCAATTACTTGGCTGGGTTTTTCTACTTACTGAATTCAGGCAATACCAAAACAAGG
  TTTCATGTTCTGAAACTGCTACTGAATTTGTCTGAAAACCTTGTCATGACAAAACGCCTACTTGTTACTG
  ATTCGGTGTCAGAATTTATGGACCTTATTAATAGGGAGGAGTCAGATGAAAACATTCAAATTGTTCTTGC
  AATATTTGAGACTATCAGTAAACATATTCAAAAAGAGGCACTGTTTTCTGATGATGATGATGATGATGAA
  GAAGAAGATGCAGTGAATCTTGAACCATTTATTTCTGCATTCCGTGAGGCTGAAAAAATTGCCAAGGAAC
  TAAAACGCAAACCTGGCAATCAAAAAGCACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211032 representing BC027187
  Red=Cloning site Green=Tags(s)
  
  MESGPKATSKSMPVAKEDEVVIGSWFWADDEEINLQADDESIFGSWFWGTGENSLRSVGVNCEKMPKAGE
  KEVTDSWFWAGDVNTEAEVEEQARSASTKATIFVPWFWSEKQPNMDLGSEPCSDIMAGAEEEPIIGPWFW
  AKVDNSVEAEVNSKSSLEDEEEPIRSPWFGAREQTDMKYAAGIRYKPMAEAEDANKKSCVWAKEPCLYPT
  NRECLKSTLGEKEDTVDPWLWSNNYPRTKTITGSWLWAAEEGNIDDETGEKIKLPTLEDNAFNSWFWKEN
  EESIVEAPKREEFRPEAEEEDIIGSWFWAGDEDRFEPAAKINEENKIASEDEDTVGSWFWGNEEASLEAV
  RRGTFESAPGIKEEKVTGSWFWTDKAKVGAGSQTVETGSETEEEAIFESLIWAAKKDSIQAGVKRVSKPK
  DDGNIAVGSWLWSSDKATKEAKTLIVSEASPENGKESVVKFGSRAKDEVINKTGSGDNCKHSTEAETIVG
  AWFWEGDEASFESNPVPVCKAVCEPESSAEHEPDPSRRPQSWDEVTVQFKAGPWGKAGFPPMNPFRFPKE
  AASLFAEMFGGKPKLVEVGPEREPEPQFPFQYDPSYRSVREIREHLKARESAQPENWSCNCIQCELRIGS
  EEFEELLLLMDRNRDPFIHEISKIAMGMRGASQFTRDFIRNSGVVSLIEALLNYPSSRVRTRFLENMVRM
  APPYPDLNMIETYVCQICEDTFDYDLDSPDQLSGLTMITHLTATSDYHKVVVNYLAGFFYLLNSGNTKTR
  FHVLKLLLNLSENLVMTKRLLVTDSVSEFMDLINREESDENIQIVLAIFETISKHIQKEALFSDDDDDDE
  EEDAVNLEPFISAFREAEKIAKELKRKPGNQKAP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027187
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC027187.1
• RefSeq Size: 3139
• RefSeq ORF: 2624
• Locus ID: 67298