Hmgcr (NM_008255) Mouse Tagged ORF Clone

CAT#: MG211067

Hmgcr (GFP-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (Hmgcr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hmgcr
• Synonyms: HMG-CoAR; Red
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211067 representing NM_008255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCAAGACTTTTCCGGATGCATGGCCTCTTCGTGGCCTCCCACCCCTGGGAAGTTATTGTGGGAA
  CAGTGACACTTACAATCTGTATGATGTCCATGAACATGTTCACCGGCAACAACAAGATCTGTGGCTGGAA
  TTATGAGTGCCCCAAATTTGAAGAGGACGTGCTGAGCAGCGACATCATCATCCTGACGATAACGCGGTGC
  ATCGCCATCCTGTACATTTACTTCCAGTTCCAGAATCTACGGCAGCTTGGGTCCAAGTACATTCTGGGTA
  TTGCTGGCCTCTTCACAATTTTCTCAAGCTTTGTCTTCAGTACAGTCGTCATTCATTTCCTCGACAAAGA
  ACTGACAGGCTTAAATGAAGCTTTGCCCTTTTTTCTGCTCTTGATTGACCTTTCTAGAGCGAGTGCATTA
  GCAAAGTTTGCCCTCAGTTCAAATTCACAGGATGAAGTAAGGGAAAATATAGCTCGTGGAATGGCAATCC
  TGGGCCCCACATTCACTCTTGACGCTCTTGTGGAATGCCTTGTGATTGGAGTTGGCACCATGTCAGGCGT
  CCGCCAGCTGGAGATCATGTGCTGCTTCGGCTGCATGTCAGTGTTGGCCAACTACTTTGTGTTCATGACA
  TTCTTCCCGGCCTGTGTGTCGCTGGTCCTAGAGCTTTCTCGTGAAAGCCGTGAGGGTCGTCCAATTTGGC
  AGCTCAGCCATTTTGCCAGAGTTTTAGAAGAAGAAGAAAATAAACCAAACCCCGTAACCCAAAGGGTCAA
  GATAATTATGTCTTTAGGCTTGGTCCTTGTTCACGCTCATAGTCGCTGGATAGCTGATCCTTCTCCTCAG
  AACAGCACAGCGGAGCAGGCTAAGGTTTCCTTGGGTCTGGACGAAGATGTGTCCAAGAGAATTGAACCAA
  GTGTTTCTCTCTGGCAGTTTTATCTCTCCAAGATGATCAGCATGGACATTGAGCAAGTGATTACCCTGAG
  TTTAGCCTTCCTTTTGGCTGTCAAGTATATTTTCTTTGAACAAGCAGAGACAGAATCGACACTGTCATTA
  AAAAATCCTATCACGTCTCCCGTCGTGACCTCAAAGAAAGCTCAAGACAACTGTTGTAGACGTGAGCCTC
  TGCTTGTGAGAAGGAACCAGAAGCTTTCGTCAGTAGAGGAGGATCCAGGAGCGAACCAAGAGAGAAAAGT
  TGAGGTTATAAAACCATTAGTGGTGGAAGCAGAGACTACAAGCAGAGCTACGTTTGTGCTTGGGGCTTCT
  GTAGCCAGCCCTCCATCCGCCCTGGGGACACAGGAGCCTGGAATTGAACTCCCCATCGAGCCACGACCTA
  ATGAAGAATGTCTGCAGATACTGGAGAATGCAGAGAAAGGTGCAAAGTTCCTTAGTGATGCAGAGATCAT
  CCAGTTGGTCAATGCCAAGCATATCCCAGCTTACAAATTGGAAACTCTAATGGAAACTCATGAACGTGGT
  GTGTCTATTCGCCGGCAGCTCCTCTCCACAAAGCTTGCAGAGCCGTCCTCTCTACAGTACCTGCCTTACA
  GAGATTATAATTATTCCTTGGTGATGGGAGCTTGCTGTGAGAATGTGATCGGATATATGCCCATCCCTGT
  TGGAGTGGCAGGGCCTCTGTGCCTGGATGGGAAGGAGTACCAGGTGCCGATGGCAACGACGGAAGGCTGT
  CTTGTGGCCAGCACTAACAGAGGCTGCAGAGCCATAAGCCTTGGTGGAGGTGCCAGCAGCCGGGTCCTTG
  CAGATGGGATGACTCGAGGCCCAGTGGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTAAAGACCTG
  GCTTGAAACACCTGAAGGGTTTGCAGTGATAAAGGAGGCCTTTGATAGCACCAGCAGATTTGCTCGTCTA
  CAGAAACTCCACGTGACGATGGCAGGACGCAACCTCTATATCCGTTTCCAGTCCAGGACGGGGGACGCCA
  TGGGCATGAACATGATCTCTAAGGGTACGGAGAAAGCACTGCTGAAGCTTCAGGAGTTCTTTCCTGACAT
  GCAGATTCTGGCAGTCAGTGGGAACTATTGCACCGACAAGAAGCCTGCTGCCATAAACTGGATCGAAGGA
  CGAGGAAAGACTGTGGTTTGTGAAGCCGTCATTCCAGCCAAGGTGGTGAGAGAGGTGTTAAAGACAACTA
  CGGAAGCTATGGTTGACGTAAACATTAACAAGAATCTTGTGGGCTCGGCCATGGCTGGGAGCATAGGCGG
  CTACAACGCCCACGCAGCAAACATTGTCACTGCTATCTACATCGCATGTGGCCAGGATGCAGCACAGAAT
  GTGGGGAGTTCAAACTGTATTACTTTAATGGAAGCCAGTGGTCCCACAAATGAAGACTTATATATCAGCT
  GCACCATGCCATCGATAGAGATAGGAACCGTGGGTGGTGGGACCAACCTTCTACCTCAGCAAGCCTGCCT
  GCAGATGCTAGGTGTTCAAGGAGCATGCAAAGACAATCCTGGAGAAAACGCACGGCAGCTTGCCCGAATT
  GTATGTGGCACTGTGATGGCTGGTGAGCTGTCCTTGATGGCAGCCTTGGCAGCAGGACATCTTGTCAGAA
  GTCACATGGTTCACAACAGATCAAAGATAAATTTACAAGATCTTCAAGGAACGTGCACCAAGAAGGCAGC
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211067 representing NM_008255
  Red=Cloning site Green=Tags(s)
  
  MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRC
  IAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASAL
  AKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMT
  FFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKIIMSLGLVLVHAHSRWIADPSPQ
  NSTAEQAKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLAFLLAVKYIFFEQAETESTLSL
  KNPITSPVVTSKKAQDNCCRREPLLVRRNQKLSSVEEDPGANQERKVEVIKPLVVEAETTSRATFVLGAS
  VASPPSALGTQEPGIELPIEPRPNEECLQILENAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERG
  VSIRRQLLSTKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGC
  LVASTNRGCRAISLGGGASSRVLADGMTRGPVVRLPRACDSAEVKTWLETPEGFAVIKEAFDSTSRFARL
  QKLHVTMAGRNLYIRFQSRTGDAMGMNMISKGTEKALLKLQEFFPDMQILAVSGNYCTDKKPAAINWIEG
  RGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQN
  VGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARI
  VCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008255
• ORF Size: 2661 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008255.1, NP_032281.1
• RefSeq Size: 3996
• RefSeq ORF: 2664
• Locus ID: 15357
• Gene Summary: Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins. [UniProtKB/Swiss-Prot Function]