Gria1 (NM_008165) Mouse Tagged ORF Clone

CAT#: MG211142

Gria1 (GFP-tagged) - Mouse glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gria1
• Synonyms: 2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; HIPA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211142 representing NM_008165
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTACATCTTTGCCTTTTTCTGCACCGGTTTTCTAGGTGCGGTTGTGGGTGCCAATTTCCCCAACA
  ATATCCAGATAGGGGGATTATTTCCAAACCAACAATCACAGGAACATGCGGCTTTTAGGTTTGCTTTGTC
  ACAACTCACGGAGCCCCCCAAGCTGCTTCCCCAGATCGATATTGTGAACATCAGCGACAGCTTTGAGATG
  ACTTACCGATTCTGTTCCCAGTTCTCCAAAGGAGTGTACGCCATCTTTGGATTTTATGAACGAAGGACTG
  TCAACATGCTGACCTCCTTCTGTGGGGCCCTCCATGTGTGCTTCATCACTCCAAGTTTTCCCGTTGACAC
  ATCCAATCAGTTTGTCCTTCAGCTGCGCCCGGAACTACAGGAAGCTCTCATTAGCATTATCGACCATTAC
  AAGTGGCAGACTTTTGTCTACATTTATGATGCTGACCGGGGCCTGTCAGTCCTGCAGAGAGTCTTGGATA
  CAGCCGCCGAGAAGAACTGGCAGGTGACGGCTGTCAACATTCTAACAACCACGGAGGAAGGATACCGGAT
  GCTCTTTCAGGACCTGGAGAAGAAAAAGGAGAGGCTGGTGGTGGTGGACTGTGAATCAGAACGCCTCAAC
  GCCATCCTGGGCCAGATTGTGAAGCTAGAAAAGAACGGCATCGGGTACCACTACATCCTCGCCAACCTGG
  GCTTCATGGACATTGACTTAAATAAGTTCAAGGAGAGTGGAGCCAATGTGACAGGTTTCCAACTGGTGAA
  CTACACAGACACGATCCCAGCCAGAATCATGCAGCAGTGGAGGACAAGTGACGCTCGGGACCACACCAGG
  GTGGACTGGAAGAGGCCAAAGTACACTTCTGCTCTTACCTATGATGGTGTGAAGGTGATGGCGGAGGCCT
  TCCAGAGCCTGCGGAGGCAGAGGATTGACATATCCCGGCGAGGGAATGCTGGGGACTGTCTGGCTAACCC
  AGCTGTGCCCTGGGGCCAAGGGATCGACATCCAGAGAGCCCTGCAGCAGGTGCGCTTTGAAGGTTTGACA
  GGAAATGTGCAGTTTAACGAGAAAGGGCGCCGGACCAACTACACCCTCCATGTGATCGAAATGAAGCATG
  ATGGAATCCGCAAGATTGGTTACTGGAATGAAGATGATAAATTTGTCCCCGCAGCCACGGACGCTCAGGC
  TGGAGGGGACAACTCAAGCGTCCAGAATAGAACCTACATCGTCACGACTATCCTCGAAGATCCTTACGTG
  ATGCTTAAAAAGAATGCCAACCAATTTGAAGGCAATGACCGCTATGAGGGCTACTGCGTGGAACTGGCTG
  CGGAGATCGCCAAGCACGTGGGCTATTCCTACCGACTTGAGATTGTCAGCGACGGCAAATACGGAGCCCG
  GGATCCTGACACAAAGGCCTGGAATGGCATGGTGGGAGAGCTAGTCTATGGAAGAGCAGATGTGGCGGTG
  GCCCCCTTGACCATAACCTTGGTCCGGGAGGAAGTCATCGACTTCTCCAAGCCATTCATGAGTTTGGGAA
  TCTCCATTATGATTAAGAAGCCACAGAAGTCCAAGCCAGGTGTCTTCTCCTTTCTTGACCCTTTGGCCTA
  CGAGATCTGGATGTGTATAGTGTTTGCCTACATTGGAGTGAGCGTCGTCCTCTTCCTGGTCAGCCGTTTC
  AGTCCTTATGAATGGCACAGTGAAGAGTTTGAAGAAGGACGAGATCAGACAACCAGTGACCAGTCAAATG
  AGTTTGGCATATTCAACAGCCTGTGGTTCTCGCTGGGGGCCTTCATGCAGCAAGGATGTGACATTTCCCC
  CAGGTCCCTGTCTGGACGCATCGTCGGCGGTGTCTGGTGGTTCTTCACTTTGATTATCATCTCCTCATAC
  ACAGCCAACCTGGCTGCCTTCCTGACTGTGGAAAGGATGGTGTCTCCCATCGAGAGTGCAGAGGACCTGG
  CAAAGCAGACGGAAATTGCTTATGGGACATTGGAAGCAGGATCCACTAAGGAGTTCTTCAGGAGGTCTAA
  AATCGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCTGCAGAACCGTCTGTGTTTGTTCGGACCACA
  GAGGAGGGCATGATCAGAGTGAGAAAGTCTAAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGAATG
  AGTACATTGAGCAACGCAAGCCCTGTGACACCATGAAAGTGGGAGGTAACTTGGATTCCAAAGGCTATGG
  CATTGCAACACCCAAGGGGTCCGCCCTGAGAAATCCAGTAAACCTGGCAGTGTTAAAACTGAACGAGCAG
  GGGCTTTTGGACAAATTGAAAAACAAATGGTGGTACGACAAGGGCGAGTGCGGCAGCGGGGGAGGTGACT
  CCAAGGACAAGACCAGTGCTCTGAGCCTGAGCAATGTGGCAGGCGTGTTCTACATCCTGATTGGAGGGCT
  GGGATTGGCCATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGCGAGTCGAAGCGGATGAAG
  GGTTTCTGTTTGATTCCACAGCAATCCATCAATGAAGCCATACGGACATCGACCCTCCCCAGGAACAGCG
  GGGCAGGAGCCAGCGGAGGAAGTGGCAGTGGAGAGAATGGCAGAGTGGTCAGCCAGGACTTCCCCAAGTC
  CATGCAATCCATTCCCTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACAGGATTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211142 representing NM_008165
  Red=Cloning site Green=Tags(s)
  
  MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEM
  TYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHY
  KWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLN
  AILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTR
  VDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLT
  GNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYV
  MLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAV
  APLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRF
  SPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSY
  TANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTT
  EEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQ
  GLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMK
  GFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008165
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008165.2, NP_032191.2
• RefSeq Size: 5434
• RefSeq ORF: 2724
• Locus ID: 14799
• Gene Summary: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. [UniProtKB/Swiss-Prot Function]