Pld2 (BC068317) Mouse Tagged ORF Clone

CAT#: MG211273

Pld2 (GFP-tagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pld2
• Synonyms: PLD1C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211273 representing BC068317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTAACCCAGAAGAACCTCTTTCCCTATGGGGACTATCTGAACTCCAGCCAGTTGCACATGGAGC
  CAGATGAGGTTGACACTCTGAGGGAAGGAGAGGATCCAGCTGATCGAATGCATCCCTATCTGGCCATCTA
  TGACCTTCAGCCTCTGAAAGCACACCCCTTGGTGTTCGCCCCTGGGGTCCCTGTTATAGCCCAGGTGGTG
  GGCACCGAAAGATACACCAGCGGATCCAAGGTGGGAACCTGTACTCTATATTCTGTTCGCTTGACGCATG
  GTGACTTTACCTGGACAACCAAGAAGAAGTTCCGACACTTTCAGGAGCTGCATCGGGACCTCCAGAGACA
  CAAAGTCTTGATGAGTCTGCTCCCTTTGGCTCGCTTTGCTGTGACCCATTCTCCAGCCCGAGAGGCAGCC
  GCCGAGGATATACCCTCCCTACCCCGAGGAGGTTCTGAGGGCTCTGCCAGACACACAGCCAGCAAACAGA
  AATACTTGGAAAATTACCTCAACCGCCTCCTGACCATGTCTTTCTATCGCAATTACCACGCCATGACAGA
  ATTTCTGGAAGTCAGTCAACTTTCCTTTATCCCAGACCTTGGCTCCAAAGGACTGGAAGGGGTGATCCGG
  AAGCGCTCGGGCGGGCATCGAGTTCCCGGCTTCACCTTCTGTGGCCGAGACCAAGTTTGTTATCGATGGT
  CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGCGCCCGGAGACCGGCGCCATCTCATT
  TGTTCAGCTTTTTGACCCTGGCTTTGAGGTCCAGGTCGGAAAAAGGAGCACAGAGACGCGGTATGGGGTG
  AGGATCGACACCTCCCACAGGTCCCTGATTCTCAAATGCAGCAGCTACCGGCAGGCACGGTGGTGGGGCC
  AGGAGATCACGGAGCTGGCACAGGGTTCGGGCAGAGATTTTCTACAGCTACATCAGCATGACAGCTATGC
  CCCACCCCGGCCCGGCACCCTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT
  GCCATCCTGCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAAATTTACCTGAAGC
  GTCCAGCCCATTCCGACGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAAGAAGGTGTCCGAGT
  TTCCATACTGCTGTTTAAGGAAGTGGAGCTGGCCTTGGGCATCAACAGTGGCTACAGCAAGAGGACGCTG
  ATGCTGCTGCATCCCAACATAAAGGTGATGCGACACCCAGACCTTGTGACACTGTGGGCTCATCACGAGA
  AGCTCCTGGTGGTAGACCAAGTGGTGGCATTCTTGGGCGGGCTGGACCTGGCCTTCGGCCGCTGGGATGA
  CGTGCAATACCGACTGACTGACCTGGGTGACCCCTCTGAACCTGTACATTTACAGACTCCCACACTAGGT
  TCAGACCCTGCAGCCACTCCAGACCTCTCGCATAACCAATTCTTCTGGCTGGGAAAGGACTACAGCAACC
  TCATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTTGAAGATTTCATCGACAGGGAGACCACACCCAG
  GATGCCATGGAGGGATGTTGGAGTGGTTGTACACGGAGTAGCTGCCAGGGACCTTGCCCGGCACTTCATC
  CAGCGCTGGAATTTCACCAAGACCACCAAGGCCAGGTATAAGACACCTTTGTACCCCTACCTGCTGCCCA
  AGTCCACCAGCACTGCAAACAATCTCCCCTTCATGATCCCAGGCGGGCAGTGTGCCACTGTGCAGGTCTT
  GAGGTCTGTGGATCGATGGTCAGCAGGGACATTGGAGAACTCCATCCTCAATGCCTACCTACATACCATT
  CGAGAGAGCCAGCACTTTCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGAACAGTTC
  TGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCTCACGAACAGGGGCAGTGTTTCCGAGT
  CTACTTGCTTCTGCCTTTGCTCCCTGGCTTTGAGGGGGACATCTCCACAGGGGGTGGTAACTCCATCCAG
  GCTATTCTGCACTTCACCTACAGCCTCTGCCTCCTTCACCCTTTCTTCTCTCTCAGGACCCTGTGTCGTG
  GGGAACATTCAATCCTACATCGTCTCAAAGCAGCCATGGGGACTGCGTGGCGAGATTACATGTCCATCTG
  TGGGCTTCGCACCCATGGAGAGCTGGGCGGGCACCCAATCTCTGAGCTCATCTATATCCACAGCAAGATG
  CTCATTGCGGATGACAGAACAGTCATCATTGGTTCTGCGAACATCAATGACAGGAGCTTGCTGGGGAAGC
  GTGACAGTGAGCTAGCCATCCTGATCAAGGACACAGAAATGGAACCATCCCTCATGGATGGGGTGGAGTA
  CCAGGCAGGCAGATTTGCCTTGAGTTTGCGGAAGCACTGTTTCAGTGTGATTCTTGGGGCAAATACCTGG
  CCAGACCTGGATCTCCGAGACCCTGTCTGTGATGACTTCTTCCAGCTGTGGCAAGAAACAGCGGAGAACA
  ATGCCACCATCTATGAGCAGATCTTCCGCTGCCTGCCGTCCAATGCTACCCGTTCCCTGCGGGCTCTCCG
  GGAGTATGTGGCTGTGGAGTCCTTGGCTACAGTCAGCCCTTCTTTGGCTCAGTCTGAGCTTGCCCACATC
  CAGGGCCACCTAGTTCACTTCCCCCTCAAGTTTCTGGAGGACGAGTCCTTGTTGCCCCCACTGGGGAGTA
  AAGAAGGGATGATACCTTTAGAAGTGTGGACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211273 representing BC068317
  Red=Cloning site Green=Tags(s)
  
  MTVTQKNLFPYGDYLNSSQLHMEPDEVDTLREGEDPADRMHPYLAIYDLQPLKAHPLVFAPGVPVIAQVV
  GTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLPLARFAVTHSPAREAA
  AEDIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIR
  KRSGGHRVPGFTFCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTETRYGV
  RIDTSHRSLILKCSSYRQARWWGQEITELAQGSGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVAD
  AILRAQEEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTL
  MLLHPNIKVMRHPDLVTLWAHHEKLLVVDQVVAFLGGLDLAFGRWDDVQYRLTDLGDPSEPVHLQTPTLG
  SDPAATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFI
  QRWNFTKTTKARYKTPLYPYLLPKSTSTANNLPFMIPGGQCATVQVLRSVDRWSAGTLENSILNAYLHTI
  RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYLLLPLLPGFEGDISTGGGNSIQ
  AILHFTYSLCLLHPFFSLRTLCRGEHSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKM
  LIADDRTVIIGSANINDRSLLGKRDSELAILIKDTEMEPSLMDGVEYQAGRFALSLRKHCFSVILGANTW
  PDLDLRDPVCDDFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHI
  QGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC068317
• ORF Size: 2832 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC068317.1
• RefSeq Size: 3671
• RefSeq ORF: 2834
• Locus ID: 18806
• Gene Summary: This gene is a member of the phospholipase D (PLD) superfamily. The encoded protein catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. Phosphatidic acid is an essential intracellular lipid second messenger for many signaling pathways and has been implicated in a variety of physiological processes including cytoskeletal organization and cell proliferation. A similar gene in human may also function as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]