Gtf2i (BC053044) Mouse Tagged ORF Clone

CAT#: MG211413

Gtf2i (GFP-tagged) - Mouse general transcription factor II I (cDNA clone MGC:62296 IMAGE:5704935)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gtf2i
• Synonyms: TFII-I, WBSCR6, BAP-135, GtfII-I, Spin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211413 representing BC053044
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAAGTAGTGATGTCTGCCTTGCCTGCCGAAGATGAAGAGTCTTCAGAGAGCAGGATGGTGGTGA
  CCTTCCTCATGTCTGCCCTGGAGTCCATGTGTAAAGAGCTGGCCAAGTCCAAGGCTGAGGTGGCCTGCAT
  TGCTGTGTATGAGACCGACGTGTTTGTTGTTGGAACCGAAAGAGGGCGTGCTTTTGTCAATACCAGAAAA
  GATTTCCAAAAAGACTTTGTGAAGTATTGTGTTGAAGAAGAAGAAAAGGCCGCCGAGATGCATAAGATGA
  AATCTACCACCCAGGCCAATCGGATGAGTGTGGATGCTGTAGAAATTGAAACACTCAGAAAAACGGTGGA
  GGACTATTTCTGTTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTCCCTGTGCCATATGAGAAG
  ATGCTGCGCGACCAGTCGGCTGTGGTGGTGCAGGGGCTTCCCGAGGGGGTTGCCTTCAAGCACCCCGAAC
  ATTACGACCTCGCAACCTTGAAGTGGATTTTGGAGAACAAGGCAGGGATTTCCTTCATCATTAAGAGACC
  TTTCCTCGAGCCGAAGAAACACCTCGGTGGTCGAGTGCTGGCGGCCGAGGCTGAGAGGTCCATGCTGTCT
  CCTAGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCTCTGGAAA
  TGGCCGCTGTGACAGTGAAGGAGGAGTCAGAAGACCCTGATTACTATCAGTATAACATTCAAGGCCCTTC
  TGAAACTGATGGTGTTGATGAAAAGCTCCCCCTTTCGAAGGCTTTGCAAGGAAGCCATCACTCCTCAGAA
  GGCAACGAGGGAACGGAAGTGGAAGTGCCAGCAGAAGATTCTACTCAACATGTCCCTTCAGAAACCAGTG
  AGGACCCCGAAGTTGAGGTGACCATTGAAGATGATGACTATTCTCCACCCACCAAGAGGCTAAAGAGCAC
  GGAGCCGCCCCCGCCCCCGCCCGTCCCGGAGCCCGCCAACGCTGGCAAGCGCAAAGTGAGGGAGTTCAAC
  TTTGAGAAATGGAACGCACGCATCACTGACCTACGGAAACAAGTTGAAGAGTTGTTCGAAAGAAAATATG
  CTCAAGCCATCAAAGCCAAAGGCCCGGTGACGATCCCGTACCCTCTTTTCCAGTCCCATGTTGAAGATCT
  TTACGTAGAAGGGCTTCCTGAAGGGATTCCTTTTAGAAGGCCGTCGACGTACGGCATTCCTCGCCTTGAG
  AGGATATTGCTGGCGAAGGAAAGGATCCGCTTCGTGATTAAGAAACATGAGCTTCTGAACTCAACACGAG
  AAGATTTACAGCTTGATAAACCAGCCTCAGGAGTGAAAGAAGAGTGGTACGCTCGCATCACCAAACTGAG
  GAAGATGGTTGACCAGCTCTTCTGCAAGAAATTTGCCGAGGCCTTGGGAAGCACGGAAGCCAAGGCTGTA
  CCGTACCAAAAATTTGAGGCCCATCCCAATGATCTCTATGTGGAAGGACTTCCAGAAAACATTCCTTTCC
  GAAGCCCCTCGTGGTATGGAATCCCAAGACTGGAAAAAATCATCCAAGTGGGCAATCGAATTAAATTTGT
  TATCAAGAGACCAGAGCTGCTCACTCACAGCACAACTGAAGTGACTCAGCCACGGACAAACACACCAGTC
  AAAGAAGATTGGAATGTCAGAATCACCAAGCTCCGAAAGCAAGTGGAAGAGATCTTTAATTTGAAGTTTG
  CTCAGGCTCTGGGCCTCACAGAGGCTGTGAAGGTGCCATATCCTGTGTTCGAATCCAACCCGGAGTTTCT
  GTATGTGGAAGGGCTCCCCGAAGGGATCCCCTTCCGAAGCCCTACCTGGTTTGGGATTCCACGCCTCGAG
  AGGATTGTCCGTGGCAGCAATAAGATCAAGTTTGTTGTTAAAAAGCCTGAGCTAGTTGTGTCCTATTTGC
  CTCCTGGGATGGCTAGCAAAATCAACACTAAAGCATTGCAGTCCCCAAAACGACCACGAAGCCCTGGGAG
  CAACTCCAAGGTTCCTGAAATTGAGGTCACTGTGGAAGGCCCCAACAACAGCAGTCCTCAGACCTCTGCG
  GTTCGAACTCCTACCCAGACCAATGGTTCAAATGTTCCCTTCAAGCCTCGAGGGAGAGAGTTTTCCTTTG
  AGGCCTGGAATGCTAAGATCACAGACCTGAAGCAGAAAGTGGAGAACCTCTTCAATGAAAAGTGCGGGGA
  AGCGCTTGGCCTTAAGCAGGCTGTGAAAGTCCCGTTTGCGTTGTTCGAGTCCTTCCCAGAAGACTTCTAT
  GTGGAAGGCTTGCCCGAGGGGGTGCCCTTCCGAAGGCCGTCCACATTTGGCATTCCCAGGCTGGAGAAGA
  TACTCCGGAACAAGGCCAAGATAAAGTTCATCATTAAAAAACCTGAGATGTTCGAGACGGCCATCAAAGA
  GAGCACCTCTTCCAAAAGCCCTCCAAGAAAGATAAACTCATCACCCAACGTTAATACTACTGCATCAGGT
  GTGGAAGACCTGAACATCATCCAGGTGACAATTCCAGATGACGATAATGAACGACTGTCCAAAGTTGAAA
  AGGCCAGGCAGCTGCGGGAGCAGGTCAACGACCTCTTCAGTCGGAAGTTTGGTGAAGCTATTGGGATGGG
  CTTCCCGGTGAAGGTCCCCTACAGGAAGATCACCATCAACCCTGGCTGCGTGGTGGTCGATGGCATGCCC
  CCCGGGGTGTCCTTCAAAGCCCCCAGTTACCTGGAGATCAGCTCCATGAGGAGGATCTTAGACTCTGCGG
  AGTTTATCAAGTTCACTGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGCTGGTTGATCAGAA
  CGAGTCTGAAGGCCCTGTGATACAAGAATCAGCCGAGGCAAGCCAGTTGGAAGTCCCAGTAACAGAAGAA
  ATTAAAGAGACAGATGGGAGTTCACAGATCAAGCAGGAGCCAGACCCCACGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211413 representing BC053044
  Red=Cloning site Green=Tags(s)
  
  MAQVVMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK
  DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK
  MLRDQSAVVVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVLAAEAERSMLS
  PSGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGPSETDGVDEKLPLSKALQGSHHSSE
  GNEGTEVEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPTKRLKSTEPPPPPPVPEPANAGKRKVREFN
  FEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLE
  RILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAV
  PYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPV
  KEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLE
  RIVRGSNKIKFVVKKPELVVSYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNSSPQTSA
  VRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFY
  VEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASG
  VEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMP
  PGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPVTEE
  IKETDGSSQIKQEPDPTW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053044
• ORF Size: 2996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC053044, AAH53044
• RefSeq Size: 4461
• RefSeq ORF: 2996
• Locus ID: 14886