Llgl1 (BC055399) Mouse Tagged ORF Clone

CAT#: MG211565

Llgl1 (GFP-tagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (cDNA clone MGC:62651 IMAGE:6308689)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Llgl1
• Synonyms: Mgl1, Llglh
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211565 representing BC055399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTTAAGCAGGAGCTCTTCG
  CCTTCCACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCTTGGCCTTCGATCCTGAGCTCCG
  CATCATGGCCATCGGCACCAGATCTGGGGCCGTCAAGATCTATGGTGCACCCGGAGTGGAATTTACAGGC
  CTACATCGGGACGCAGCCACCGTCACCCAGATGCATTTCCTCCCTGGTCAGGGCCGCCTCCTGACCCTGC
  TAGATGACAGCAGCTTGCATCTCTGGGAGATCATCCATCATAATGGCTGCGCCCACCTGGAGGAAGGCCT
  CAGCTTCCACCCACCCAGCAGACCCAGTTTTGACAATGCCAGTTTCCCTGCCAGTCTAACACGTGTCACT
  GTGGTCCTGCTCGTAGCTGGCAATACAGCAGCCCTGGGAACTGAGAGTGGTAGCATATTCTTCCTGGATG
  TAGCCACCCTGGCACTGCTGGAGGGGCAGACTCTCAGCCCAGATGTGGTTCTGCGCAGTGTGCCAGATGA
  TTACCGGTGTGGAAAGGCCTTGGGCCCTGTGGAGTCACTCCAGGGACATCTGCAAGACCCCAGCAAGATC
  CTCATAGGCTACAGTCGGGGTTTACTGGTCATCTGGAGCCAGGCCACACAGTCTGTGGACAACGTTTTCC
  TAGGTAACCAGCAGCTGGAGAGCCTGTGTTGGGGCCGTGATGGCAGCAGCATTATCAGCTCACACAGTGA
  TGGCAGCTATGCCATCTGGTCCACAGACACTGGCAGCCCCCCAACGCTGCAGCCCACTGTAGTGACCACA
  CCCTACGGCCCCTTCCCCTGCAAGGCCATCAACAAGATTCTGTGGCGGAGCTGTGAGTCAGGAGACCACT
  TTATCATCTTCAGTGGTGGCATGCCTCGAGCCAGCTATGGTGACCGCCACTGTGTGAGTGTACTGCGAGC
  AGAGACACTGGTGACCCTGGACTTCACCTCTCGTGTCATTGACTTCTTCACGGTGCACAGCACACAGCCA
  GAGGATGAGTGTGACAACCCCCAGGCCTTAGCCGTGCTTCTGGAGGAGGAGCTGGTGGTGCTGGACCTGC
  AGACACCAGGCTGGCCAGCTGTGCCCGCCCCTTACCTGGCCCCACTGCATTCGTCAGCTATCACCTGCTC
  TGCCCATGTTGCCAATGTCCCCAGCAAGCTGTGGGCCCGCATTGTAAGTGCTGGTGAGCAGCAGAGCCCA
  CAGCCTGCCTCCAGTGCCTTGAGTTGGCCCATTACCGGGGGCCGGAACTTGGCCCAGGAACCCTCGCAGC
  GTGGGCTGCTGCTCACTGGCCATGAGGATGGCACTGTGCGGTTCTGGGACGCCTCTGGTGTGGCGCTAAG
  GCCACTCTACAAACTGAGCACAGCTGGCCTCTTCCAGACGGACTGTGAACATGCTGACAGCCTGGCCCAG
  GCTGTGGAGGATGACTGGCCGCCCTTCCGCAAGGTGGGCTGCTTTGATCCCTACAGTGATGACCCCCGGC
  TAGGAATCCAGAAGGTTGCGCTTTGCAAGTACACAGCCCAGATGGTGGTAGCTGGCACTGCAGGCCAGGT
  GCTGGTGCTGGAGCTCAGTGAGGTCCCAGCAGAGCATGCCGTCAGTGTGGCCAACGTGGATCTTCTTCAG
  GATCGCGAGGGCTTCACGTGGAAGGGTCACGAGCGGCTGAACCCACACACGGGGCTGCTGCCGTGGCCTG
  CCGGATTCCAGCCCCGCATGCTGATACAGTGCCTCCCACCCGCCGCTGTCACTGCTGTCACACTCCATGC
  TGAGTGGAGCCTCGTGGCCTTTGGTACCAGTCATGGCTTTGGCCTTTTTGACTACCAGCGCAAGAGCCCT
  GTGCTGGCTAGGTGCACCCTTCACCCCAATGATTCTTTGGCCATGGAGGGGCCACTGTCACGGGTGAAGT
  CCCTCAAGAAGTCACTGAGACAGTCATTCCGGCGAATCCGCAAGAGCCGTGTCTCAGGCAAAAAACGGAC
  TCCTGCTGCCAGTAGCAAGTTGCAGGAGGCCAATGCCCAGCTGGCCGAGCAGACCTGCCCACACGACCTG
  GAGATGACACCCGTGCAGCGCCGCATTGAGCCTCGGTCTGCTGACGACTCGCTCTCCGGTGTTGTACGCT
  GCCTCTACTTTGCTGACACGTTCCTTCGAGATGCGACCCACCACGGGCCCACCATGTGGGCGGGCACCAA
  CTCGGGCTCTGTGTTCGCCTATGCGCTGGAGGTTCCAGCAGCCACAGCAGGCGGAGAGAAGCGGCCTGAG
  CAGGCAGTGGAGGCGGTGCTGGGCAAGGAGGTGCAGCTAATGCACCGAGCACCTGTGGTGGCCATTGCTG
  TGCTGGATGGTCGTGGCCGGCCACTGCCTGAGCCCTATGAGGCTTCCCGGGACCTGGCCCAGGCGCCAGA
  CATGCAAGGCGGCCATGCTGTGCTCATTGCATCTGAGGAACAATTCAAGGTGTTCACACTACCCAAGGTG
  AGTGCTAAGACTAAATTCAAGCTTACAGCCCATGAAGGCTGTCGTGTGCGGAAGGTAGCCCTGGCTACAT
  TTGCCAGCGTGATGTCTGAGGACTATGCCGAGACCTGCCTTGCCTGCCTCACCAACCTGGGTGATGTCCA
  CGTCTTCTCGGTGCCTGGCCTGAGGCCTCAGGTGCACTACTCCTGTATCCGGAAGGAGGACATCAGTGGC
  ATCGCTTCCTGTGTCTTCACACGTCACGGCCAGGGCTTTTACTTGATTTCTCCATCGGAATTTGAGCGCT
  TCTCACTGAGTGCTCGCAACATCACAGAACCACTATGTTCTCTGGATATAAGCTGGCCCCAAAATGCCAC
  CCAGCCCAGGCTTCAAGAGTCACCCAAGCTGAGCCAGGCTAATGGGACCAGAGACATCATTCTGGCCCCA
  GAGAGCTGCGAAGGAAGCCCTAGCTCTGCCCACAGCAAGCGAGCTGATACCATGGAACCACCCGAGGCCG
  CTCTCTCGCCTGTGTCCATTGATTCAGCTGCTAGTGGGGATACCATGCTGGACACAACAGGGGATGTCAC
  CGTGGAATATGTGAAGGATTTTCTGGGCTCTCCTGAGGACTCTGAGAAGAACCTGCGGAACCTGGAGGCA
  GATGATGCTTGTCGGGCCTACACCCTTCTGATTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211565 representing BC055399
  Red=Cloning site Green=Tags(s)
  
  MMKFRFRRQGADPQREKLKQELFAFHKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTG
  LHRDAATVTQMHFLPGQGRLLTLLDDSSLHLWEIIHHNGCAHLEEGLSFHPPSRPSFDNASFPASLTRVT
  VVLLVAGNTAALGTESGSIFFLDVATLALLEGQTLSPDVVLRSVPDDYRCGKALGPVESLQGHLQDPSKI
  LIGYSRGLLVIWSQATQSVDNVFLGNQQLESLCWGRDGSSIISSHSDGSYAIWSTDTGSPPTLQPTVVTT
  PYGPFPCKAINKILWRSCESGDHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRVIDFFTVHSTQP
  EDECDNPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVANVPSKLWARIVSAGEQQSP
  QPASSALSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLAQ
  AVEDDWPPFRKVGCFDPYSDDPRLGIQKVALCKYTAQMVVAGTAGQVLVLELSEVPAEHAVSVANVDLLQ
  DREGFTWKGHERLNPHTGLLPWPAGFQPRMLIQCLPPAAVTAVTLHAEWSLVAFGTSHGFGLFDYQRKSP
  VLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRTPAASSKLQEANAQLAEQTCPHDL
  EMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDATHHGPTMWAGTNSGSVFAYALEVPAATAGGEKRPE
  QAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPKV
  SAKTKFKLTAHEGCRVRKVALATFASVMSEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDISG
  IASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDISWPQNATQPRLQESPKLSQANGTRDIILAP
  ESCEGSPSSAHSKRADTMEPPEAALSPVSIDSAASGDTMLDTTGDVTVEYVKDFLGSPEDSEKNLRNLEA
  DDACRAYTLLIK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC055399
• ORF Size: 3188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC055399, AAH55399
• RefSeq Size: 4320
• RefSeq ORF: 3188
• Locus ID: 16897
• Gene Summary: Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane. [UniProtKB/Swiss-Prot Function]