Nlrp5 (BC053384) Mouse Tagged ORF Clone

CAT#: MG211648

Nlrp5 (GFP-tagged) - Mouse NLR family, pyrin domain containing 5 (cDNA clone MGC:60553 IMAGE:30049556)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nlrp5
• Synonyms: PAN11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211648 representing BC053384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCCTCCAGAAAAAGAAAGTAAAGCAATCTTGAAAGCACGTGGATTGGAAGAGGAACAGAAGTCAG
  AAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCAGAAAGACCAAGGACCAGAGCAGGAGCA
  GACATCAGAAAGCACAATGGGTCCTCCAGAAAAAGACAGTAAAGCAATCTTGAAAGCACGTGGATTGGAA
  GAGGAACAGAAGTCAGAAAGCACAATGTCTCCTTCAGAAAATGTCAGTAGAGCAATCCTGAAAGACAGTG
  GATCAGAAGAAGTGGAACAGGCGTCAGAAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCA
  GAAAGACCAAGGACCAGAGCAGGAGCAGACATCAGAAACCTTACAATCTAAGGAAGAAGATGAAGTGACA
  GAGGCAGATAAAGATAATGGAGGTGACTTACAAGACTACAAGGCCCATGTGATTGCTAAGTTCGACACAA
  GTGTGGATCTACACTATGACAGCCCAGAGATGAAATTATTGTCTGATGCTTTTAAACCATACCAGAAAAC
  CTTCCAGCCTCACACCATTATCCTACATGGAAGACCAGGAGTTGGGAAGTCAGCTTTGGCCAGAAGTATT
  GTTCTTGGCTGGGCACAGGGTAAACTCTTCCAAAAAATGTCCTTTGTCATCTTCTTCTCTGTTAGAGAAA
  TAAAGTGGACAGAGAAGAGCAGTTTGGCACAGCTGATTGCTAAGGAGTGTCCAGACTCCTGGGATCTAGT
  GACAAAGATCATGTCCCAACCAGAAAGACTCTTGTTTGTCATAGATGGCTTGGATGATATGGACTCTGTC
  CTCCAACATGATGATATGACACTATCCAGAGACTGGAAGGATGAACAGCCCATATACATCCTGATGTACA
  GCCTCCTGAGGAAGGCTCTCCTACCTCAGTCCTTTCTCATCATTACCACCAGAAACACAGGCTTAGAAAA
  ACTCAAGTCAATGGTTGTGTCCCCCCTCTATATACTGGTTGAAGGACTGTCTGCATCAAGGAGATCTCAG
  CTGGTCCTCGAGAACATCTCCAATGAGTCTGATAGAATACAAGTCTTCCATTCTCTGATAGAAAATCACC
  AGCTGTTTGACCAATGCCAGGCCCCCTCTGTGTGCTCCCTGGTCTGTGAGGCTCTACAGCTACAGAAGAA
  ACTGGGAAAGAGATGCACCCTACCCTGCCAGACTCTCACCGGTTTGTATGCCACGTTGGTGTTTCACCAG
  CTCACCTTGAAAAGGCCTTCCCAGAGCGCTCTCAGTCAGGAAGAACAGATTACTCTAGTGGGTTTGTGCA
  TGATGGCAGCTGAAGGAGTGTGGACCATGAGGTCGGTATTCTATGATGATGACCTGAAGAACTATAGCCT
  AAAGGAGTCTGAGATCTTGGCCCTCTTTCACATGAACATCCTTCTCCAGGTTGGCCACAACAGTGAGCAG
  TGTTATGTTTTCTCCCACCTCAGCCTGCAGGATTTCTTTGCTGCCTTATATTATGTTTTAGAAGGGCTGG
  AGGGATGGAATCAGCATTTTTGCTTCATTGAAAACCAAAGGAGCATCATGGAGGTGAAGAGAACTGACGA
  CACTCGCCTCCTCGGGATGAAGCGTTTCTTATTTGGCCTCATGAACAAGGATATCTTGAAGACTCTGGAG
  GTTCTGTTTGAATATCCCGTGATTCCAACTGTTGAGCAGAAGCTCCAACACTGGGTCTCTCTGATAGCTC
  AGCAGGTCAATGGCACCAGCCCAATGGACACCCTGGATGCCTTCTATTGTCTATTTGAGTCTCAGGATGA
  AGAGTTTGTTGGCGGGGCTCTCAAACGCTTCCAAGAAGTGTGGCTGCTGATTAACCAGAAGATGGACTTG
  AAGGTCTCTTCCTACTGTCTCAAGCACTGTCAGAACTTGAAGGCAATCCGGGTGGATATCAGAGACCTCC
  TCTCGGTAGATAATACTCTCGAGCTGTGCCCTGTTGTTACTGTCCAGGAGACACAATGTAAGCCCCTCCT
  CATGGAGTGGTGGGGAAACTTCTGCTCTGTGCTTGGCAGCCTCCGGAACTTGAAGGAGCTGGACTTGGGC
  GACAGCATCCTGAGTCAACGGGCCATGAAGATACTGTGCCTCGAGCTGCGGAATCAGTCCTGCAGAATAC
  AGAAGCTGACGTTTAAGAGTGCAGAGGTAGTGTCTGGCCTGAAACATCTCTGGAAGCTCCTTTTTAGCAA
  TCAAAACTTAAAGTACCTCAATCTAGGGAACACTCCCATGAAGGATGATGACATGAAGTTAGCCTGCGAA
  GCGCTGAAACATCCAAAGTGCTCCGTGGAGACTCTGAGGTTGGATTCCTGTGAGTTAACCATCATTGGTT
  ATGAGATGATCTCCACGCTTCTTATTTCAACCACCAGGCTAAAGTGTCTCAGCCTGGCCAAAAATAGAGT
  GGGAGTAAAAAGCATGATATCCCTTGGGAATGCCTTGAGTAGCTCAATGTGTCTACTGCAAAAGTTGATA
  CTGGACAACTGTGGCCTCACACCTGCCAGCTGCCACCTTCTGGTCTCAGCCCTTTTCAGCAACCAGAACT
  TGACACACCTGTGCCTGTCAAACAACAGCCTGGGGACTGAAGGAGTGCAACAGCTGTGTCAGTTCCTGAG
  GAATCCAGAATGTGCTCTCCAGCGGCTGATACTGAATCACTGCAACATTGTAGATGATGCTTATGGCTTC
  CTGGCAATGAGACTTGCAAACAACACAAAGCTGACCCACCTGAGCCTGACCATGAACCCCGTAGGGGATG
  GTGCAATGAAGCTACTGTGTGAAGCTTTAAAGGAACCTACTTGTTACCTTCAAGAACTGGAACTAGTGGA
  CTGCCAACTCACACAGAACTGCTGCGAGGACCTGGCCTGTATGATCACAACAACCAAGCACTTAAAAAGT
  TTGGATCTTGGTAACAACGCCCTGGGTGACAAAGGAGTCATAACCCTGTGTGAGGGACTGAAGCAAAGTA
  GCAGCTCCCTGAGGAGACTTGGGTTGGGGGCATGTAAGTTGACTTCCAATTGCTGTGAGGCATTGTCATT
  GGCCATCTCTTGCAACCCTCACCTGAACAGCCTAAACCTGGTGAAGAATGACTTCAGTACATCGGGGATG
  TTGAAGCTGTGCTCTGCGTTCCAATGCCCTGTCTCTAACCTGGGGATAATTGGCCTGTGGAAGCAGGAGT
  ACTATGCCCGAGTGAGAAGACAGCTGGAGGAAGTTGAGTTTGTCAAGCCCCACGTGGTGATTGATGGTGA
  TTGGTATGCTAGTGATGAAGATGACCGAAACTGGTGGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211648 representing BC053384
  Red=Cloning site Green=Tags(s)
  
  MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLE
  EEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSETLQSKEEDEVT
  EADKDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSI
  VLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSV
  LQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQ
  LVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQ
  LTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQ
  CYVFSHLSLQDFFAALYYVLEGLEGWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLE
  VLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDL
  KVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLG
  DSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACE
  ALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLI
  LDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGF
  LAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKS
  LDLGNNALGDKGVITLCEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGM
  LKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053384
• ORF Size: 3335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC053384, AAH53384
• RefSeq Size: 3534
• RefSeq ORF: 3335
• Locus ID: 23968
• Gene Summary: This gene encodes a member of the NACHT, leucine-rich repeat, and pyrin domain containing family. Members of this family have a pyrin domain at the N-terminus, a central NACHT domain, and a C-terminal leucine-rich repeat domain. This gene encodes a maternal-effect factor that is essential for early embryonic development in the mouse. Homozygous null mutant females are sterile, and embryos die following the first cleavage. This gene is required for endoplasmic reticulum redistribution and calcium homeostasis in oocytes. In addition, ovulated oocytes mutant for this gene have abnormal mitochondrial localization and increased mitochondrial activity, which results in mitochondrial damage and early embryonic lethality. Pseudogenes of this gene have been found on chromosomes 7 and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]