Polr3b (NM_027423) Mouse Tagged ORF Clone

CAT#: MG211682

Polr3b (GFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (Polr3b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr3b
• Synonyms: 2700078H01Rik; A330032P03Rik; C128; C85372; RPC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211682 representing NM_027423
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTTCTAGCCGAGGAGTTCGGGAGCCTGACTCCGGAGCAGCTGACTGCGCCGATCCCGACTGTGG
  AGGAAAAATGGAGGCTGCTTCCAGCATTTTTAAAGGTTAAAGGCCTGGTGAAACAGCACATAGACTCCTT
  TAACTATTTCATTAATGTAGAGATAAAGAAGATAATGAAAGCCAATGAAAAGGTTACAAGTGATGCTGAC
  CCCATGTGGTACTTAAAATACCTTAATATCTATGTCGGACTTCCTGATGTTGAAGAAAGCTTCAATGTAA
  CTAGACCAGTGTCCCCTCATGAGTGCCGTCTGAGGGACATGACGTACTCCGCCCCAATCACAGTGGACAT
  TGAGTATACCCGAGGCAGCCAGAGGATAATCCGCAACGCCTTACCCATTGGCAGAATGCCCATCATGCTC
  CGAAGCTCGAACTGTGTTCTAACAGGGAAAACGCCGGCAGAGTTTGCCAAACTGAATGAGTGTCCACTAG
  ACCCAGGTGGCTACTTCATTGTTAAAGGAGTGGAAAAAGTCATTCTTATCCAAGAGCAGCTGTCCAAGAA
  CAGGATCATTGTGGAGGCCGACAGGAAAGGAGCCGTTGGAGCCTCAGTTACCAGCTCCACCCATGAGAAG
  AAAAGCAGAACCAACATGGCCGTGAAACAGGGTCGATTCTATCTGAGGCACAACACCCTGTCTGAAGATA
  TCCCCATTGTCATCATATTTAAGGCCATGGGTGTGGAGAGTGACCAGGAAATCGTGCAGATGATTGGAAC
  AGAGGAGCACGTGATGGCTGCATTTGGGCCCAGCTTGGAGGAGTGCCAGAAAGCTCAGATCTTCACCCAG
  ATGCAGGCATTGAAGTATATAGGGAATAAAGTAAGAAGACAAAGAATGTGGGGAGGTGGACCAAAGAAAA
  CCAAAATAGAAGAAGCAAGAGAACTCCTGGCTTCTACCATTCTGACCCACGTACCAGTGAAGGAATTCAA
  TTTCCGAGCTAAGTGTATCTACACTGCAGTGATGGTGCGAAGGGTGATCCTGGCCCAAGGAGATAACAAG
  GTCGATGACAGAGACTATTATGGCAACAAACGACTGGAATTGGCAGGACAGCTCTTATCTCTTCTTTTTG
  AAGATTTGTTTAAAAAGTTTAATTCAGAAATGAAGAAGATTGCAGACCAAGTGATTCCTAAACAGAGAGC
  AGCCCAGTTTGACGTCGTTAAACACATGCGCCAAGACCAGATTACCAACGGCATGGTCAATGCCATCTCC
  ACGGGAAATTGGTCTCTGAAGAGATTTAAGATGGACCGACAGGGTGTGACCCAGGTGCTGTCTCGCTTGT
  CATATATATCTGCACTCGGCATGATGACAAGAATCTCTTCCCAGTTTGAAAAGACGAGGAAAGTGAGTGG
  CCCTCGTTCTCTGCAGCCGTCTCAGTGGGGAATGCTCTGTCCCTCCGACACACCTGAAGGGGAGGCCTGT
  GGTTTGGTTAAGAACTTGGCCCTCATGACACATATAACAACTGACATGGAAGATGGACCCATTATTAAGT
  TAGCTGGGAACTTGGGAGTAGAAGATGTGAATTTATTATGTGGAGAAGAGCTCTCTTACCCAAATGTGTT
  TCTTGTCTTCCTGAATGGTAACATCCTTGGTGTCATTCGAGACCATAAAAAGCTTGTCAGCACATTCCGG
  CTGATGCGAAGAGCAGGCTATATCAATGAATTTGTTTCTATCTCCACAAACCTTACGGATCGCTGTGTTT
  ATATTTCCTCTGATGGCGGGCGCTTGTGCAGGCCTTACATAATTGTTAAGAAACAAAAGCCAGCTGTCAC
  AAATAAGCACATGGAAGAGTTGGCTCAAGGCTACAGGAATTTTGAAGATTTCTTACATGAAAGTCTAGTT
  GAATACTTAGATGTGAATGAAGAAAATGACTGTAACATTGCGCTCTACGAACATACAATTAATAAAGACA
  CCACCCACTTGGAGATTGAACCCTTCACTCTGCTTGGTGTTTGTGCCGGCCTGATCCCATACCCTCATCA
  CAACCAGTCTCCAAGAAACACCTACCAGTGTGCTATGGGCAAGCAAGCCATGGGTACCATTGGCTACAAT
  CAACGGAACAGAATTGATACACTTATGTATCTACTAGCATATCCACAAAAGCCCATGGTTAAAACAAAAA
  CAATCGAGTTGATAGATTTTGAGAAGTTGCCGGCTGGCCAGAACGCAACTGTTGCCGTGATGAGCTACAG
  CGGCTATGACATTGAGGACGCGCTGGTTTTGAACAAGGCCTCTCTGGACAGAGGTTTTGGGCGTTGTCTT
  GTGTATAAAAATGCTAAGTGCACCTTGAAACGATACACCAATCAGACCTTTGATAAGGTGATGGGTCCCA
  TGTTGGATGCTGCTACCAGAAAACCCATCTGGCGACATGAAATTTTAGATGCAGATGGCATTTGTTCTCC
  AGGTGAGAAAGTGGAAAACAAACAGGTGCTTGTAAACAAGTCCATGCCCACAGTGACACAGATTCCTTTG
  GAAGGGAGCAACGTGCCACAGCAACCACAGTATAAAGATGTGCCTATCACGTACAAAGGTGCGACAGACT
  CCTACATCGAGAAAGTGATGATCTCTTCCAACGCCGAGGATGCTTTCCTCATCAAGATGCTCCTGAGACA
  GACGCGGCGCCCAGAGATTGGAGACAAATTCAGCAGTCGCCATGGACAAAAGGGTGTCTGTGGCCTGATC
  GTCCCCCAGGAAGACATGCCGTTCTGTGATTCTGGCATCTGTCCCGACATCATAATGAACCCACACGGCT
  TCCCATCAAGAATGACGGTTGGAAAGTTAATTGAGCTGCTGGCTGGCAAGGCAGGCGTGTTAGACGGCAG
  GTTCCACTACGGCACTGCCTTTGGAGGGAGTAAAGTGAAGGATGTGTGTGAAGATCTCGTTCGCCATGGT
  TACAACTACCTGGGGAAGGACTACGTCACCTCAGGCATCACAGGTGAGCCCTTAGAAGCATACATCTATT
  TTGGACCTGTGTATTATCAGAAACTGAAACACATGGTGCTGGATAAAATGCACGCCCGGGCCCGAGGACC
  ACGAGCTGTCCTTACCAGGCAGCCCACTGAAGGGCGGTCGCGCGATGGTGGCTTGCGACTTGGAGAAATG
  GAACGCGACTGTCTAATCGGCTATGGAGCGAGTATGCTTTTGCTGGAGAGACTAATGATCTCAAGTGATG
  CCTTTGAGGTTGACGTCTGCGGGCAGTGTGGACTCCTGGGATATTCTGGCTGGTGCCACTACTGCAAATC
  TTCGTGCCACGTGTCCTCCCTCCGCATCCCGTATGCCTGCAAGCTGCTCTTCCAGGAGTTACAGTCCATG
  AACATCATCCCCAGGCTGAAACTGGCCAAGTACAACGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211682 representing NM_027423
  Red=Cloning site Green=Tags(s)
  
  MDVLAEEFGSLTPEQLTAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDAD
  PMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIML
  RSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEK
  KSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQ
  MQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNK
  VDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAIS
  TGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEAC
  GLVKNLALMTHITTDMEDGPIIKLAGNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVSTFR
  LMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLV
  EYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYN
  QRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCL
  VYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPL
  EGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLI
  VPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHG
  YNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEM
  ERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSM
  NIIPRLKLAKYNE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027423
• ORF Size: 3399 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027423.1, NP_081699.1
• RefSeq Size: 4858
• RefSeq ORF: 3402
• Locus ID: 70428