Srebf1 (BC056922) Mouse Tagged ORF Clone

CAT#: MG211688

Srebf1 (GFP-tagged) - Mouse sterol regulatory element binding factor 1 (cDNA clone MGC:66503 IMAGE:6824948)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Srebf1
• Synonyms: D630008H06, ADD-1, SREBP-1, SREBP-1a, SREBP-1c, SREBP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211688 representing BC056922
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGCTGGCCTTCGGTGAGGCGGCTCTGGAACAGACACTGGCCGAGATGTGCGAACTGGACACAG
  CGGTTTTGAACGACATCGAAGACATGCTCCAGCTCATCAACAACCAAGACAGTGACTTCCCGGGCCTGTT
  TGACGCCCCCTATGCTGGGGGTGAGACAGGGGACACAGGCCCCAGCAGCCCAGGTGCCAACTCTCCTGAG
  AGCTTCTCTTCTGCTTCTCTGGCCTCCTCTCTGGAAGCCTTCCTGGGAGGACCCAAGGTGACACCTGCAC
  CCTTGTCCCCTCCACCATCGGCACCCGCTGCTTTAAAGATGTACCCGTCCGTGTCCCCCTTTTCCCCTGG
  GCCTGGGATCAAAGAGGAGCCAGTGCCACTCACCATCCTACAGCCTGCAGCGCCACAGCCGTCACCGGGG
  ACCCTCCTGCCTCCGAGCTTCCCCGCACCACCCGTACAGCTCAGCCCTGCGCCCGTGCTGGGTTACTCGA
  GCCTGCCTTCAGGCTTCTCAGGGACCCTTCCAGGAAACACTCAGCAGCCACCATCTAGCCTGCCGCTGGC
  CCCTGCACCAGGAGTCTTGCCCACCCCTGCCCTGCACACCCAGGTCCAAAGCTTGGCCTCCCAGCAGCCG
  CTGCCAGCCTCAGCAGCCCCTAGAACAAACACTGTGACCTCACAGGTCCAGCAGGTCCCAGTTGTATTGC
  AGCCACACTTCATCAAGGCAGACTCACTGCTGCTGACAGCTGTGAAGACAGATGCAGGAGCCACCGTGAA
  GACTGCAGGCATCAGCACCCTGGCTCCTGGCACAGCCGTGCAGGCAGGTCCCCTGCAGACCCTGGTGAGT
  GGAGGGACCATCTTGGCCACAGTACCTTTGGTTGTGGACACAGACAAACTGCCCATCCACCGACTCGCAG
  CTGGCAGCAAGGCCCTAGGCTCAGCTCAGAGCCGTGGTGAGAAGCGCACAGCCCACAATGCCATTGAGAA
  GCGCTACCGGTCTTCTATCAATGACAAGATTGTGGAGCTCAAAGACCTGGTGGTGGGCACTGAAGCAAAG
  CTGAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATCCGCTTCTTGCAGCACAGCAACCAGAAGC
  TCAAGCAGGAGAACCTGACCCTACGAAGTGCACACAAAAGCAAATCACTGAAGGACCTGGTGTCAGCTTG
  TGGCAGTGGAGGAGGCACAGATGTGTCTATGGAGGGCATGAAACCCGAAGTGGTGGAGACGCTTACCCCT
  CCACCCTCAGACGCCGGCTCACCCTCCCAGAGTAGCCCCTTGTCTTTTGGCAGCAGAGCTAGCAGCAGTG
  GTGGTAGTGACTCTGAGCCCGACAGTCCAGCCTTTGAGGATAGCCAGGTCAAAGCCCAGCGGCTGCCTTC
  ACACAGCCGAGGCATGCTGGACCGCTCCCGCCTGGCCCTGTGTGTACTGGCCTTTCTGTGTCTGACCTGC
  AATCCTTTGGCCTCGCTTTTCGGCTGGGGCATTCTCACTCCCTCTGATGCTACGGGTACACACCGTAGTT
  CTGGGCGCAGCATGCTGGAGGCAGAGAGCAGAGATGGCTCTAATTGGACCCAGTGGTTGCTGCCACCCCT
  AGTCTGGCTGGCCAATGGACTACTAGTGTTGGCCTGCTTGGCTCTTCTCTTTGTCTATGGGGAACCTGTG
  ACTAGGCCACACTCTGGCCCGGCTGTACACTTCTGGAGACATCGCAAACAAGCTGACCTGGATTTGGCCC
  GGGGAGATTTCCCCCAGGCTGCTCAACAGCTGTGGCTGGCCCTGCAAGCGCTGGGCCGGCCCCTGCCCAC
  CTCAAACCTGGATCTGGCCTGCAGTCTGCTTTGGAACCTCATCCGCCACCTGCTCCAGCGTCTCTGGGTG
  GGCCGCTGGCTGGCAGGCCAGGCCGGGGGCCTGCTGAGGGACCGTGGGCTGAGGAAGGATGCCCGTGCCA
  GTGCCCGGGATGCGGCTGTTGTCTACCATAAGCTGCACCAGCTGCATGCCATGGGCAAGTACACAGGAGG
  ACATCTTGCTGCTTCTAACCTGGCACTAAGTGCCCTCAACCTGGCTGAGTGCGCAGGAGATGCTATCTCC
  ATGGCAACACTGGCAGAGATCTATGTGGCAGCGGCCCTGAGGGTCAAAACCAGCCTCCCAAGAGCCCTGC
  ACTTCTTGACACGTTTCTTCCTGAGCAGCGCCCGCCAGGCCTGCCTAGCACAGAGCGGCTCGGTGCCTCT
  TGCCATGCAGTGGCTCTGCCACCCTGTAGGTCACCGTTTCTTTGTGGACGGGGACTGGGCCGTGCACGGT
  GCCCCCCCGGAGAGCCTGTACAGCGTGGCTGGGAACCCAGTGGATCCGCTGGCCCAGGTGACCCGGCTAT
  TCCGTGAACATCTCCTAGAGCGAGCGTTGAACTGTATTGCTCAGCCCAGCCCAGGGGCAGCTGACGGAGA
  CAGGGAGTTCTCAGATGCCCTTGGATATCTGCAGTTGCTAAATAGCTGTTCTGATGCTGCCGGGGCTCCT
  GCTTGCAGTTTCTCTGTCAGCTCCAGCATGGCTGCCACCACTGGCCCAGACCCAGTGGCCAAGTGGTGGG
  CCTCACTGACAGCTGTGGTGATCCACTGGCTGAGGCGGGATGAAGAGGCAGCTGAGCGCTTGTACCCACT
  GGTAGAGCATATCCCCCAGGTGCTGCAGGACACTGAGAGACCCCTGCCCAGGGCAGCTCTGTACTCCTTC
  AAGGCTGCCCGGGCTCTGCTGGACCACAGAAAGGTGGAATCTAGCCCAGCCAGCCTGGCCATCTGTGAGA
  AGGCCAGTGGGTACCTGCGGGACAGCTTAGCCTCTACACCAACTGGCAGTTCCATTGACAAGGCCATGCA
  GCTGCTCCTGTGTGATCTACTTCTTGTGGCCCGTACCAGTCTGTGGCAGCGGCAGCAGTCACCAGCTTCA
  GTCCAGGTAGCTCACGGTACCAGCAATGGACCCCAGGCCTCTGCTCTGGAGCTGCGTGGTTTCCAACATG
  ACCTGAGCAGCCTGCGGCGGTTGGCACAGAGCTTCCGGCCTGCTATGAGGAGGGTATTCCTACATGAGGC
  CACAGCTCGGCTGATGGCAGGAGCAAGTCCTGCCCGGACACACCAGCTCCTGGATCGCAGTCTGAGGAGG
  AGGGCAGGTTCCAGTGGCAAAGGAGGCACTACAGCTGAGCTGGAGCCACGGCCCACATGGCGGGAGCACA
  CCGAGGCCCTGCTGTTGGCATCCTGCTATCTGCCCCCTGCCTTCCTGTCGGCTCCTGGGCAGCGAATGAG
  CATGCTGGCCGAGGCGGCACGCACCGTAGAGAAGCTTGGCGATCACCGGCTACTGCTGGACTGCCAGCAG
  ATGCTCCTGCGCCTGGGCGGCGGAACCACCGTCACTTCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211688 representing BC056922
  Red=Cloning site Green=Tags(s)
  
  MDELAFGEAALEQTLAEMCELDTAVLNDIEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGANSPE
  SFSSASLASSLEAFLGGPKVTPAPLSPPPSAPAALKMYPSVSPFSPGPGIKEEPVPLTILQPAAPQPSPG
  TLLPPSFPAPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQPPSSLPLAPAPGVLPTPALHTQVQSLASQQP
  LPASAAPRTNTVTSQVQQVPVVLQPHFIKADSLLLTAVKTDAGATVKTAGISTLAPGTAVQAGPLQTLVS
  GGTILATVPLVVDTDKLPIHRLAAGSKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAK
  LNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTP
  PPSDAGSPSQSSPLSFGSRASSSGGSDSEPDSPAFEDSQVKAQRLPSHSRGMLDRSRLALCVLAFLCLTC
  NPLASLFGWGILTPSDATGTHRSSGRSMLEAESRDGSNWTQWLLPPLVWLANGLLVLACLALLFVYGEPV
  TRPHSGPAVHFWRHRKQADLDLARGDFPQAAQQLWLALQALGRPLPTSNLDLACSLLWNLIRHLLQRLWV
  GRWLAGQAGGLLRDRGLRKDARASARDAAVVYHKLHQLHAMGKYTGGHLAASNLALSALNLAECAGDAIS
  MATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPLAMQWLCHPVGHRFFVDGDWAVHG
  APPESLYSVAGNPVDPLAQVTRLFREHLLERALNCIAQPSPGAADGDREFSDALGYLQLLNSCSDAAGAP
  ACSFSVSSSMAATTGPDPVAKWWASLTAVVIHWLRRDEEAAERLYPLVEHIPQVLQDTERPLPRAALYSF
  KAARALLDHRKVESSPASLAICEKASGYLRDSLASTPTGSSIDKAMQLLLCDLLLVARTSLWQRQQSPAS
  VQVAHGTSNGPQASALELRGFQHDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRR
  RAGSSGKGGTTAELEPRPTWREHTEALLLASCYLPPAFLSAPGQRMSMLAEAARTVEKLGDHRLLLDCQQ
  MLLRLGGGTTVTSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC056922
• ORF Size: 3404 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC056922, AAH56922
• RefSeq Size: 4306
• RefSeq ORF: 3404
• Locus ID: 20787
• Gene Summary: This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. Alternatively spliced transcript variants have been characterized for this gene. [provided by RefSeq, Nov 2017]