Nos2 (BC062378) Mouse Tagged ORF Clone

CAT#: MG211704

Nos2 (GFP-tagged) - Mouse nitric oxide synthase 2, inducible, macrophage (cDNA clone MGC:68162 IMAGE:4978648)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nos2
• Synonyms: iNOS, NOS-II, Nos2a, i-NOS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211704 representing BC062378
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAGGAAAAGGACA
  TTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGCCCAACAATACAAGATGACCCTAAGAGTCA
  CCAAAATGGCTCCCCGCAGCTCCTCACTGGGACAGCACAGAATGTTCCAGAATCCCTGGACAAGCTGCAC
  GTGACTCCATCGACCCGTCCACAGTATGTGAGGATCAAAAACTGGGGCAGTGGAGAGATTTTGCATGACA
  CTCTTCACCACAAGGCCACATCGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCC
  CAAGAGTTTGACCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAG
  TTCATCAACCAGTATTATGGCTCCTTTAAAGAGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTG
  TAACAAAGGAAATAGAAACAACAGGAACCTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGAT
  GGCCTGGAGGAATGCCCCTCGCTGCATCGGCAGGATCCAGTGGTCCAACCTGCAGGTCTTTGACGCTCGG
  AACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACTTTATGCCACCAACAATGGCA
  ACATCAGGTCGGCCATCACTGTGTTCCCCCAGCGGACTGACGGCAAACATGACTTCAGGCTCTGGAATTC
  ACAGCTCATCCGGTACGCTGGCTACCAGATGCCCGATGGCACCATCAGAGGGGATGCTGCCACCTTGGAG
  TTCACCCAGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCT
  TGCAAGCTGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGA
  GCATCCCAAGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACATG
  CTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGATTG
  GAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGAAGTGGGCCGAAGGATGGGCCTGGA
  GACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGGAGATCAATGTGGCTGTGCTCCATAGT
  TTCCAGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGA
  ATGAGTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCAT
  CACCCCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGATCGAGCCC
  TGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAGAGAGATCCGATTTAGAGTCTTGG
  TGAAAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCT
  CTTTGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTC
  AACACCAAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAACTACTGCTGGTGG
  TGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGACTCTGAAGAAATCTCTGTTCATGCT
  TAGAGAACTCAACCACACCTTCAGGTATGCTGTGTTTGGCCTTGGCTCCAGCATGTACCCTCAGTTCTGC
  GCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTGGGAGCCTCTCAGCTTGCCCCAACAGGAGAAG
  GGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCTGGGCTGTACAAACCTTCCGGGCAGCCTGTGA
  GACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAACGCTTCACTTCCAATGCAACATGGGAG
  CCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCTCAACAGAGCCCTCAGCAGCATCCATG
  CAAAGAATGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGCCGCACCAC
  CCTCCTCGTTCAGCTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATC
  TTCCCAGGCAACCAGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACC
  AAACTGTGTGCCTGGAGGTTCTGGATGAGAGCGGCAGCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTG
  CTCACTCAGCCAAGCCCTCACCTACTTCCTGGACATTACGACCCCTCCCACCCAGCTGCAGCTCCACAAG
  CTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAGAGATTGGAGGCCTTGTGTCAGCCCTCAGAGTACA
  ATGACTGGAAGTTCAGCAACAACCCCACGTTCCTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCC
  CGCTGCCTTCCTGCTGTCGCAGCTCCCTATCTTGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGAC
  CACACCCCCTCGGAGGTTCACCTCACTGTGGCCGTGGTCACCTACCGCACCCGAGATGGTCAGGGTCCCC
  TGCACCATGGAGTCTGCAGCACTTGGATCAGGAACCTGAAGCCCCAGGACCCAGTGCCCTGCTTTGTGCG
  AAGTGTCAGTGGCTTCCAGCTCCCTGAGGACCCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGC
  ATTGCTCCCTTCCGAAGTTTCTGGCAGCAGCGGCTCCATGACTCCCAGCACAAAGGGCTCAAAGGAGGCC
  GCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTCTATCAGGAAGAAATGCAGGAGAT
  GGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGCTGCCCGGCAAACCCAAGGTCTAC
  GTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCCACGGGGAGCAGGGCCACC
  TCTACGTTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACGTTGAAGAAGCTGGTGGCCACCAA
  GCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGAGCCAGAAACGTTATCATGAA
  GATATCTTCGGTGCAGTCTTTTCCTATGGGGCAAAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGA
  GGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211704 representing BC062378
  Red=Cloning site Green=Tags(s)
  
  MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH
  VTPSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIE
  FINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDAR
  NCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRTDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLE
  FTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANM
  LLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHS
  FQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEP
  WKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAF
  NTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFC
  AFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWE
  PQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGI
  FPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHK
  LARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQD
  HTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTG
  IAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVY
  VQDILQKQLANEVLSVLHGEQGHLYVCGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHE
  DIFGAVFSYGAKKGSALEEPKATRL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC062378
• ORF Size: 3435 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC062378, AAH62378
• RefSeq Size: 4112
• RefSeq ORF: 3437
• Locus ID: 18126
• Gene Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]