Heph (BC048237) Mouse Tagged ORF Clone

CAT#: MG211720

Heph (GFP-tagged) - Mouse hephaestin (cDNA clone MGC:54662 IMAGE:6474714)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Heph
• Synonyms: mKIAA0698, Cpl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211720 representing BC048237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCAGGCCATCTTCTCTGGGCTTTACTGTTGATGCACTCCTTGTGGTCTATACCAACTGATGGGG
  CCATTCGAAACTACTACTTGGGCATCCAGGATATGCAGTGGAACTATGCTCCCAAAGGAAGAAATGTCAT
  CACAAATCAGACTCTCAACAATGACACAGTGGCTTCCAGTTTCCTGAAGTCTGGCAAAAACAGGATAGGG
  AGTAGTTACAAGAAGACTGTTTATAAGGAATACAGTGATGGCACATACACTGAAGAAATAGCCAAGCCTG
  CCTGGTTGGGCTTCCTAGGACCACTGTTACAGGCTGAGGTGGGGGATGTCATCTTGATTCACCTGAAGAA
  TTTTGCGAGCCGACCTTACACCATTCACCCTCACGGTGTTTTTTATGAGAAGGACTCAGAAGGCTCACTA
  TACCCAGATGGTTCTTCTGGGTATCTGAAAGCGGATGATTCTGTCCCCCCTGGGGGCAGCCATGTCTACA
  ACTGGAGTATTCCAGAAAGTCATGCCCCCACTGAGGCAGACCCAGCATGCCTCACCTGGATTTACCACTC
  GCATGTAGATGCTCCAAGAGACATTGCAACTGGTCTCATTGGACCTCTTATCACCTGTAAAAGAGGGACC
  CTGGATGGTAATTCCCCACCTCAGAGGAAGGATGTGGACCATAATTTCTTCCTCCTCTTCAGTGTGATAG
  ATGAGAACCTTAGCTGGCACCTTGATGACAACATTGCTACTTACTGCTCAGACCCTGCCTCGGTGGACAA
  AGAAGATGGAGCCTTTCAAGACAGCAACAGGATGCATGCAATCAATGGGTTTGTCTTTGGGAACTTACCA
  GAGTTGAGCATGTGTGCACAGAAGCATGTGGCCTGGCACTTGTTTGGCATGGGCAATGAAATAGATGTCC
  ACACAGCTTTCTTCCATGGACAGATGCTGAGTATCCGTGGACACCACACTGATGTTGCAAACATTTTTCC
  AGCTACCTTTGTGACTGCTGAGATGGTGCCCCAAAAGTCTGGAACCTGGCTAATTAGCTGTGAAGTGAAC
  AGCCACTTGAGAAGTGGCATGCAGGCCTTCTACAAGGTTGACTCTTGCTCCATGGACCCACCTGTGGACC
  AGCTCACTGGCAAAGTTCGTCAGTACTTCATTCAGGCCCATGAGATTCAATGGGACTATGGTCCAATAGG
  GTATGATGGCAGAACTGGGAAGAGTTTGAGAGAGCCAGGAAGTGGCCCAGATAAGTACTTCCAGAAGAGC
  TCTAGTCGAATTGGAGGTACTTACTGGAAAGTTCGATATGAAGCCTTTCAAGATGAGACATTCCAGGAAA
  GGGTACATCAGGAAGAAGAAACACATCTTGGAATACTGGGACCAGTGATAAGGGCTGAAGTGGGTGACAC
  CATCCAGGTGGTCTTCTATAACCGTGCCTCCCAGCCATTCAGCATACAGCCCCATGGTGTCTTTTATGAG
  AAAAACTCTGAGGGCACCGTGTACAATGATGGCACATCTCATCCCAAAGTAGCCAAGTCATTTGAAAAAG
  TCACATACTACTGGACGGTTCCTCCCCATGCTGGGCCCACTGCTCAGGATCCTGCCTGTCTAACCTGGAT
  GTACTTCTCTGCTGCAGATCCCACAAGAGATACAAATTCTGGCCTGGTGGGCCCTCTACTGGTGTGCAAG
  GCTGGGGCCTTGGGTGCAGATGGCAAGCAGAAAGGAGTGGATAAAGAATTTTTTCTCCTCTTCACTGTGT
  TTGATGAGAATGAGAGCTGGTACAACAGTGCCAATCAGGCAGCTGGTATGTTGGATTCCCGACTGCTCTC
  AGAGGATGTCGAGGGCTTCCAGGACTCCAATCGAATGCATGCTATTAATGGATTTCTGTTCTCTAACCTG
  CCCAGGCTGGACATGTGCAAGGGTGATACTGTGGCCTGGCACCTGCTTGGCCTGGGCACAGAGACTGATG
  TACATGGGGTAATGTTCGAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAAGGTGCAGTCATGCTCTT
  TCCTCACACCTTTGTGACGGCCATCATGCAGCCTGACAATCCTGGAATATTTGAAATCTACTGCCAAGCA
  GGCAGCCACCGAGAGGAAGGGATGCAGGCAATTTATAATGTCTCTCAGTGTTCTAGTCATCAAGACAGCC
  CACGCCAACACTACCAAGCTTCAAGAGTCTACTATATCATGGCAGAAGAGATAGAGTGGGATTACTGCCC
  TGATAGAAGCTGGGAACTGGAATGGCATAACACATCTGAGAAAGACAGCTATGGCCATGTTTTCCTGAGC
  AATAAAGATGGGCTCCTGGGTTCCAAATATAAGAAAGCTGTATTCAGGGAATACACTGATGGTACTTTCA
  GAATACCTCGGCCAAGGTCTGGACCAGAGGAGCACTTGGGAATCCTGGGTCCACTTATCAGAGGAGAGGT
  TGGTGATATCTTGACTGTAGTGTTCAAGAATAAGGCCAGTCGACCATATTCTATACATGCCCATGGAGTT
  CTAGAATCTAACACTGGCGGGCCACAGGCTGCTGAGCCTGGTGAAGTACTTACTTACCAGTGGAACATCC
  CAGAAAGATCTGGTCCTGGTCCTAGTGACTCTGCTTGTGTTTCCTGGATTTATTATTCTGCAGTGGATCC
  CATCAAGGACATGTATAGTGGTCTGGTTGGACCCCTAGTCATCTGCAGAAATGGTATCTTGGAACCCAAT
  GGAGGCCGGAATGATATGGACCGGGAATTTGCCTTGTTGTTTTTGATCTTTGATGAGAACCAATCTTGGT
  ATCTGAAGGAGAATATTGCAACATATGGACCTCAAGAATCAAGTCATGTTAACTTGAAGGATGCCACCTT
  CCTAGAGAGCAATAAAATGCATGCTATCAATGGGAAACTCTATGCAAACCTCAGGGGTCTTACTGTATAC
  CAAGGAGAACGAGTAGCCTGGTACATGCTAGCCATGGGCCAAGATACTGACATTCACACTGTACACTTCC
  ATGCAGAGAGTTTCCTCTATCAGAATGGGCAAAGTTACAGGGCAGATGTGGTGGATCTCTTCCCAGGAAC
  ATTTGAAGTTGTGGAGATGGTAGCCAGCAACCCTGGGACATGGCTGATGCACTGCCATGTGACTGACCAT
  GTTCATGCTGGCATGGAGACCATCTTTACGGTCTTGTCTCATGAAGAACATTTCAGCACTATGACCACTA
  TTACTAAAGAGATTGGAAAAGTGATTCTAAGGGACATTGGAGGTGACAATGTGAAGATGCTGGGCATGAA
  CATTCCCATAAAGGATGTAGAGATTCTGTCTTCTGCTTTGATTGCCATATGTGTGCTTCTGTTGCTCATT
  GCTCTGGCTCTTGGTGGTGTAGTCTGGTACCAGCATCGACAAAGAAAGCTTCGGCGCAACAGGAGGTCCA
  TTCTTGATGATAGCTTCAAGCTTCTCTCTCTCAAGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211720 representing BC048237
  Red=Cloning site Green=Tags(s)
  
  MKAGHLLWALLLMHSLWSIPTDGAIRNYYLGIQDMQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIG
  SSYKKTVYKEYSDGTYTEEIAKPAWLGFLGPLLQAEVGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSL
  YPDGSSGYLKADDSVPPGGSHVYNWSIPESHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGT
  LDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGAFQDSNRMHAINGFVFGNLP
  ELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQMLSIRGHHTDVANIFPATFVTAEMVPQKSGTWLISCEVN
  SHLRSGMQAFYKVDSCSMDPPVDQLTGKVRQYFIQAHEIQWDYGPIGYDGRTGKSLREPGSGPDKYFQKS
  SSRIGGTYWKVRYEAFQDETFQERVHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYE
  KNSEGTVYNDGTSHPKVAKSFEKVTYYWTVPPHAGPTAQDPACLTWMYFSAADPTRDTNSGLVGPLLVCK
  AGALGADGKQKGVDKEFFLLFTVFDENESWYNSANQAAGMLDSRLLSEDVEGFQDSNRMHAINGFLFSNL
  PRLDMCKGDTVAWHLLGLGTETDVHGVMFEGNTVQLQGMRKGAVMLFPHTFVTAIMQPDNPGIFEIYCQA
  GSHREEGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWHNTSEKDSYGHVFLS
  NKDGLLGSKYKKAVFREYTDGTFRIPRPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGV
  LESNTGGPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPN
  GGRNDMDREFALLFLIFDENQSWYLKENIATYGPQESSHVNLKDATFLESNKMHAINGKLYANLRGLTVY
  QGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGQSYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDH
  VHAGMETIFTVLSHEEHFSTMTTITKEIGKVILRDIGGDNVKMLGMNIPIKDVEILSSALIAICVLLLLI
  ALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048237
• ORF Size: 3470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC048237, AAH48237
• RefSeq Size: 4544
• RefSeq ORF: 3470
• Locus ID: 15203
• Gene Summary: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. [UniProtKB/Swiss-Prot Function]