Spag9 (BC060100) Mouse Tagged ORF Clone

CAT#: MG211730

Spag9 (GFP-tagged) - Mouse sperm associated antigen 9 (cDNA clone MGC:57997 IMAGE:6417257)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spag9
• Synonyms: JLP, Jip4, Mapk8ip4, syd1, JSAP2, JSAP2a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211730 representing BC060100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCTGGATGCATGCTGCTGTTTGTGTTTGGCTTTGTTGGCGGGGCGGTGGTCATTAATTCTGCGA
  TCTTAGTATCTCTCTCTGTTTTGCTGCTTGTGCACTTTTCTATTTCTACCGGGGTGCCAGCGCTGACGCA
  GAACCTACCAAGGATACTCAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCTGCTGGAGAT
  GGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAATTTCAAGAAT
  TAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAAGGTGGATCTAAAGC
  TACCACTCCAGCTTCAACAGCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCAAAGGAAGAT
  AATGAAGGATTTGTAAAAGGCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAGAAGTCCAGG
  TTGCCCAAGAGACTAGGAATGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGTTCAAGCAAT
  CATTGAATCCACTCCTGAATTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGCACTCCCACC
  AAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCTCAGCTGGCT
  CGGGCCTTATTGGAGATGTGGATGAAGGAGCGGATTTACTAGGAATGGGTCGTGAGGTGGAGAATCTCAT
  ACTGGAGAACACACAGCTGCTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAATGACTTAATAGCCAAA
  GTGGATGAGCTGACCTGTGAGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGAAGCAAGCCAAGCTGA
  AGCTGGAGGACAAGAACAGAGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGAAGATGCAAG
  GCAAAAAGCAAAAGACGATGATGATAGTGACATTCCCACAGCCCAGAGGAAGAGGTTTACCAGAGTAGAA
  ATGGCCCGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAGCTGTTCGAT
  GGACGGAGATGATCCGGGCCTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAGGTCAAGCATCTGGCA
  ATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCCCCTGTTAACCTGAAG
  TACAACGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTCAGCTCCCTG
  GGGACAAGTCCAAGGCATTTGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGCCTCCCGCAGAGAGCA
  AAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGAGTGCAAGCTTTCGGC
  TGGAGTCTGCCTCAGAAGTACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGATGAAAAATTTACCTG
  TGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGTAGGAGTCAA
  TTTATCTGGTGGCAAGACAAGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTTTACAAGGATATTGCT
  GGTTTGGACACTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACAAGTTGGATCAAGAAC
  TCAAGGAACAGCAGAAAGAATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTGGATCTGTACCAGCAC
  CCACTCAACTACAAAGGTTATCATCATTGATGCTGTTCAGCCTGGCAACATCCTAGATAGTTTCACTGTT
  TGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACTACCCTGCAGGAGAAG
  AACTTTCTGAATCTGGTCAGGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAGTAACAGCTCCGCAGA
  GATGGACAGCTTGCTGGGAGGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTGACAGGAGCTGCCACT
  TCCCCAAGTACCAACGGTGCTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAACTGAAAATAGCGAAG
  TTGATGAAAATATTCCAACAGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGCAGGGTCCACTGAAGA
  CACTGTGGACATCTCCCAGCCTGGCGTGTACACAGAGCATGTGTTTACAGATCCACTGGGAGTTCAGATC
  CCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAATATCAGTAT
  TGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAACCATGTGGCT
  TGGAGCTCAGAATGGCTGTTTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATT
  AAGCTCAAAGACTCAATTCTCAGTATTGTACATGTGAAAGGAATTGTACTAGTGGCCCTGGCGGATGGCA
  CCCTTGCAATCTTCCACAGAGGAGTTGATGGACAATGGGATCTGTCAAACTATCACCTTTTAGACCTTGG
  ACGTCCTCATCATTCCATACGATGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAGGAACAAA
  ATCTATGTGGTTCAACCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGAAGGAGAGCC
  AAGTACGGCAGCTTGCATGGGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGATTCCACGCTCCGCCT
  CTATCATGCACACACATACCAACATCTCCAGGATGTGGATATTGAGCCTTATGTAAGCAAAATGTTAGGT
  ACTGGAAAACTGGGTTTCTCTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTTTGTGGGTGG
  GGACTGGAAATGGTGTCATTATCTCCATCCCACTGACAGAAACAAATAAAACCTCAGGGACTCCAGGTAA
  CCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAGAACAGTGATAAAGTGACTCCAGGGACATTTATA
  CCCTACTGTTCAATGGCACATGCACAGCTTTGTTTCCACGGGCACCGGGATGCTGTGAAATTCTTTGTGG
  CAGTCCCAGGTCAGGTCATTAGCCCACAAAGTAGCAGTGGTGGGGCAGATCTTACAGCTGACAAGGCAGG
  GTCATCTGCACAGGAGCCCAGTAGCCAGACGCCCTTGAAGTCCATGCTTGTCATCAGTGGAGGAGAGGGC
  TACATTGACTTCCGGATGGGTGATGAAGGTGGAGAATCTGAACTTCTGGGAGAGGATCTTCCACTGGAAC
  CTTCAGTCACCAAAGCCGAGAGGAGTCACCTGATAGTGTGGCAAGTGATGTGTGGCAATGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211730 representing BC060100
  Red=Cloning site Green=Tags(s)
  
  MNPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGD
  GLLTPDTQKGGETPGSEQWKFQELSQPRSHTSLKDELSDISQGGSKATTPASTANSDVSAIPPDTPSKED
  NEGFVKGTDTSNKSEISKHIEVQVAQETRNVSTESGENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPT
  KGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNVVKNDLIAK
  VDELTCEKDVLQGELEAVKQAKLKLEDKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVE
  MARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNATKKPEPPVNLK
  YNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFG
  WSLPQKYKQVANGQGETKMKNLPVPVYLRPLDEKDASMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDIA
  GLDTEGSKQRSASQSSLDKLDQELKEQQKEFKNQEELSSQVWICTSTHSTTKVIIIDAVQPGNILDSFTV
  CNSHVLCIASVPGARETDYPAGEELSESGQVDKASLCGSMTSNSSAEMDSLLGGITVVGCSTEGLTGAAT
  SPSTNGASPVIEKPPEMETENSEVDENIPTAEEATEATEGNAGSTEDTVDISQPGVYTEHVFTDPLGVQI
  PEDLSPVFQSSNDSDVYKDQISVLPNEQDLAREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSI
  KLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNK
  IYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLG
  TGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGTPGNRPGSVIRVYGDENSDKVTPGTFI
  PYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSGGADLTADKAGSSAQEPSSQTPLKSMLVISGGEG
  YIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMCGNE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060100
• ORF Size: 3494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC060100, AAH60100
• RefSeq Size: 4221
• RefSeq ORF: 3494
• Locus ID: 70834