Itga2 (NM_008396) Mouse Tagged ORF Clone

CAT#: MG211760

Itga2 (GFP-tagged) - Mouse integrin alpha 2 (Itga2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itga2
• Synonyms: CD49B; DX5; GPIa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211760 representing NM_008396
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCGGGACAGGCAGGGGGCGCGCTCCTGCTGCGGCTGCTAATGCTAGTTCAAGGTATTTTGAATT
  GTCTGGCGTATAATGTTGGCCTCCCAGGAGCGAAAATATTTTCCGGGCCTTCAAGTGAACAGTTTGGGTA
  TTCAGTACAGCAGCTTACGAACCCACAAGGCAACTGGCTACTGGTTGGTTCACCGTGGAGTGGCTTCCCT
  GAGAACCGAATGGGAGATGTGTACAAGTGCCCTGTGGACCTACCCACTGCCACCTGTGAAAAGCTGAATC
  TGCAAAATTCAGCAAGCATCTCAAATGTTACCGAGATAAAGACCAACATGAGCCTGGGATTGACCCTCAC
  CAGGAATCCGGGGACCGGAGGCTTTCTAACCTGTGGTCCTCTCTGGGCACATCAGTGTGGAAATCAGTAT
  TATGCAACTGGGATCTGTTCTGATGTTAGTCCAGACTTTCAGTTCTTGACCAGCTTTTCACCTGCAGTTC
  AGGCTTGCCCTTCCCTCGTGGATGTTGTAGTTGTATGTGATGAATCAAACAGTATTTATCCTTGGGAAGC
  AGTAAAGAACTTTTTGGTAAAGTTTGTGACAGGCCTGGATATAGGACCTAAAAAGACACAGGTGGCGTTA
  ATTCAATATGCCAATGAGCCGAGAATTATATTTAACTTGAACGATTTCGAAACCAAAGAGGATATGGTCC
  AGGCCACATCTGAGACGCGCCAACATGGTGGGGACCTCACAAACACCTTCAGAGCTATCGAATTCGCAAG
  AGACTACGCTTATTCACAGACTTCTGGCGGGCGCCCGGGTGCTACAAAAGTCATGGTAGTTGTGACCGAT
  GGCGAGTCCCATGATGGGTCGAAGCTGAAAACTGTGATCCAGCAATGCAATGATGACGAGATACTGAGGT
  TCGGCATAGCAGTTCTTGGGTATTTAAACAGAAATGCTCTTGATACTAAAAATTTAATCAAAGAAATAAA
  AGCAATTGCTAGTACTCCAACCGAGAGATACTTTTTCAATGTGGCCGACGAAGCGGCTCTTCTGGAGAAG
  GCTGGAACTCTAGGGGAGCAAATATTCAGCATTGAAGGCACTGTTCAAGGAGGAGACAACTTCCAGATGG
  AAATGGCACAAGTTGGATTCAGTGCAGATTACGCTCCTCAAAATGATATTCTGATGTTAGGTGCAGTGGG
  GGCTTTTGACTGGAGTGGAACTCTTGTCCAGGAGACATCTCACAAGCCCGTGATCTTTCCTAAACAAGCC
  TTTGACCAGGTTCTGCAGGATAGAAACCACAGTTCGTTTTTAGGTTACTCTGTGGCTGCAATTTCTACTG
  AAGACGGTGTCCATTTTGTTGCTGGTGCTCCTCGAGCAAATTATACTGGCCAGATAGTGTTGTACAGCGT
  TAACAAGCAAGGCAATGTGACCGTGATTCAGTCTCACAGAGGGGACCAGATTGGCTCCTATTTTGGCAGT
  GTGCTGTGCTCAGTTGATGTAGATAAAGACACCATTACAGACGTGCTCCTGGTAGGTGCTCCTACATACA
  TGAACGACCTCAAGAAAGAAGAGGGAAAAGTCTACCTATTTACCATCACAAAGGGCATCTTGAATCAGCA
  CCAGTTTCTTGAAGGGCCAGAAGGCACTGGAAATGCTCGGTTTGGTTCAGCAATTGCAGCCCTGTCAGAC
  ATCAACATGGATGGCTTTAATGATGTGATTGTCGGTTCACCTGTAGAGAATGAGAACTCTGGAGCTGTGT
  ACATTTACAATGGTCACCAGGGTACCATTCGCACCAAGTACTCCCAGAAAATCCTTGGGTCCAATGGAGC
  CTTTAGGAGACATCTCCAGTTCTTTGGAAGGTCCCTGGATGGCTATGGTGATTTAAATGGGGATTCCATC
  ACTGATGTATCCATTGGTGCTTTGGGGCAAGTGATTCAACTCTGGTCACAAAGCATTGCTGACGTGGCCA
  TAGAAGCTTTGTTCACTCCAGACAAAATCACTTTGCTCAACAAGGATGCTAAGATAACCCTAAAGCTCTG
  CTTCAGAGCAGAGTTTAGACCTGCTGGGCAAAACAATCAAGTAGCCATCCTGTTTAACATGACGCTTGAT
  GCGGACGGACATTCATCCAGGGTAACCTCCAGGGGGGTGTTCAGAGAAAACAGTGAGCGATTCTTGCAGA
  AGAATATGGTGGTAAACGAAGTGCAGAAGTGTTCTGAGCACCACATTAGCATACAGAAGCCCTCTGATGT
  TGTCAACCCTTTGGATCTGCGTGTGGACATCAGTTTGGAAAACCCAGGCACTAGCCCTGCCCTCGAAGCC
  TATTCTGAAACTGTCAAAGTCTTCAGTATCCCTTTCTATAAGGAGTGTGGCAGCGATGGAATTTGCATCT
  CTGACCTGATCCTGGATGTCCAACAGCTGCCAGCCATTCAAACTCAATCCTTTATTGTCAGTAACCAAAA
  CAAGAGGTTAACATTTTCAGTCATCCTGAAAAACAGAGGGGAAAGTGCATACAACACTGTGGTCCTGGCC
  GAGTTTTCTGAGAACTTGTTTTTTGCTTCTTTCTCCATGCCGGTCGATGGAACAGAAGTAACATGTGAGG
  TTGGTTCATCGCAGAAATCTGTTACCTGTGATGTGGGATACCCTGCCTTAAAGAGTGAACAGCAGGTGAC
  TTTTACCATTAACTTCGACTTTAATCTTCAAAACCTCCAGAACCAGGCGGCCATCAATTTCCAAGCCTTC
  AGTGAGAGCCAAGAAACAAACAAAGCAGACAATTCGGTCAGTCTCACGATTCCTCTCCTGTATGATGCCG
  AACTTCACTTAACCCGATCCACCAACATCAACTTCTATGAGATCTCTTCGGATGAGAACGCTCCTTCTGT
  CATCAAGAGTGTCGAAGATATTGGTCCTAAATTCATCTTCTCCCTCAAGGTAACAGCCGGAAGTGCCCCA
  GTGAGCATGGCATTGGTGACTATCCACATTCCTCAGTACACCAAGGAAAAGAACCCACTCCTGTATCTGA
  CTGGGATACAAACAGACCAGGCTGGCGACATCAGCTGCACAGCAGAAATAAATCCACTCAAGCTACCACA
  CACCGCCCCTTCTGTATCTTTCAAGAATGAAAACTTCCGGCATACGAAAGAATTGGACTGCAGAACCACT
  TCCTGCAGTAACATCACCTGCTGGCTGAAAGACCTTCACATGAAAGCAGAGTACTTCATCAATGTGACAA
  CCAGAGTTTGGAACAGGACCTTTGCTGCTTCGACTTTCCAGACTGTGCAGCTGACAGCAGCGGCAGAGAT
  CGATACACATAACCCTCAGCTCTTCGTGATTGAGGAAAATGCTGTCACGATTCCCCTCATGATAATGAAA
  CCCACGGAGAAAGCAGAAGTACCGACAGGGGTTATCATAGGCAGCATAATTGCGGGAATCCTTTTGCTGT
  TGGCTATGACTGCAGGTTTGTGGAAGCTCGGCTTCTTCAAAAGACAATATAAGAAAATGGGCCAAAATCC
  AGATGAGATGGATGAGACCACAGAACTCAACAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211760 representing NM_008396
  Red=Cloning site Green=Tags(s)
  
  MGPGQAGGALLLRLLMLVQGILNCLAYNVGLPGAKIFSGPSSEQFGYSVQQLTNPQGNWLLVGSPWSGFP
  ENRMGDVYKCPVDLPTATCEKLNLQNSASISNVTEIKTNMSLGLTLTRNPGTGGFLTCGPLWAHQCGNQY
  YATGICSDVSPDFQFLTSFSPAVQACPSLVDVVVVCDESNSIYPWEAVKNFLVKFVTGLDIGPKKTQVAL
  IQYANEPRIIFNLNDFETKEDMVQATSETRQHGGDLTNTFRAIEFARDYAYSQTSGGRPGATKVMVVVTD
  GESHDGSKLKTVIQQCNDDEILRFGIAVLGYLNRNALDTKNLIKEIKAIASTPTERYFFNVADEAALLEK
  AGTLGEQIFSIEGTVQGGDNFQMEMAQVGFSADYAPQNDILMLGAVGAFDWSGTLVQETSHKPVIFPKQA
  FDQVLQDRNHSSFLGYSVAAISTEDGVHFVAGAPRANYTGQIVLYSVNKQGNVTVIQSHRGDQIGSYFGS
  VLCSVDVDKDTITDVLLVGAPTYMNDLKKEEGKVYLFTITKGILNQHQFLEGPEGTGNARFGSAIAALSD
  INMDGFNDVIVGSPVENENSGAVYIYNGHQGTIRTKYSQKILGSNGAFRRHLQFFGRSLDGYGDLNGDSI
  TDVSIGALGQVIQLWSQSIADVAIEALFTPDKITLLNKDAKITLKLCFRAEFRPAGQNNQVAILFNMTLD
  ADGHSSRVTSRGVFRENSERFLQKNMVVNEVQKCSEHHISIQKPSDVVNPLDLRVDISLENPGTSPALEA
  YSETVKVFSIPFYKECGSDGICISDLILDVQQLPAIQTQSFIVSNQNKRLTFSVILKNRGESAYNTVVLA
  EFSENLFFASFSMPVDGTEVTCEVGSSQKSVTCDVGYPALKSEQQVTFTINFDFNLQNLQNQAAINFQAF
  SESQETNKADNSVSLTIPLLYDAELHLTRSTNINFYEISSDENAPSVIKSVEDIGPKFIFSLKVTAGSAP
  VSMALVTIHIPQYTKEKNPLLYLTGIQTDQAGDISCTAEINPLKLPHTAPSVSFKNENFRHTKELDCRTT
  SCSNITCWLKDLHMKAEYFINVTTRVWNRTFAASTFQTVQLTAAAEIDTHNPQLFVIEENAVTIPLMIMK
  PTEKAEVPTGVIIGSIIAGILLLLAMTAGLWKLGFFKRQYKKMGQNPDEMDETTELNS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008396
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008396.2, NP_032422.2
• RefSeq Size: 4235
• RefSeq ORF: 3537
• Locus ID: 16398
• Gene Summary: Integrin alpha-2/beta-1 is a collagen receptor, being responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides and E-cadherin. Mice homozygous for a null mutation in the alpha-2 die very early in embryogenesis. [UniProtKB/Swiss-Prot Function]