Nos3 (BC052636) Mouse Tagged ORF Clone

CAT#: MG211798

Nos3 (GFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nos3
• Synonyms: eNOS, ecNOS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211798 representing BC052636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGTTTAG
  GGCTGTGCGGCAAGCAGGGCCCAGCCTCTCCAGCACCGGAGCCTAGCCAGGCGCCAGCACCCCCGTCCCC
  AACCCGACCAGCACCAGACCACAGCCCCCCGCTAACCCGGCCCCCAGACGGACCCAGGTTTCCTCGAGTA
  AAGAATTGGGAAGTGGGCAGCATCACCTACGACACCCTCAGTGCCCAGGCTCAGCAGGATGGGCCCTGTA
  CCTCAAGACGCTGCTTGGGATCCCTGGTGTTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC
  ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCCATCAAAAGGAGT
  GGCTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAGGCTGAGGTGGCAGCCACAGGCACCTACCAGC
  TCCGGGAGAGCGAGCTGGTGTTTGGGGCCAAGCAGGCCTGGCGCAATGCTCCCCGCTGTGTGGGCCGGAT
  CCAGTGGGGAAAGCTGCAGGTATTTGATGCTCGGGACTGCAGGACTGCACAGGAAATGTTCACCTACATC
  TGTAACCACATTAAATACGCAACAAATAGAGGCAATCTTCGTTCAGCCATCACAGTGTTCCCCCAGCGCT
  GCCCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGATACGCTATGCGGGCTATAGGCAGCAGGA
  TGGCTCCGTGCGAGGGGACCCCGCCAACGTGGAGATCACTGAGCTCTGTATCCAACATGGCTGGACCCCA
  GGAAATGGCCGCTTTGATGTGCTGCCCCTGTTACTCCAGGCTCCTGATGAGCCCCCAGAACTCTTCACTC
  TGCCCCCAGAGATGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTCGAGTGGTTTGCTGCCCTTGGCCT
  GCGCTGGTATGCCCTCCCAGCTGTGTCCAACATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCTGCTGCC
  CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGCTACAACA
  TACTTGAGGATGTGGCTGTGTGCATGGATCTGGACACCAGGACAACCTCATCCCTGTGGAAAGACAAGGC
  AGCGGTGGAAATTAATGTGGCCGTGTTGCACAGTTACCAGCTGGCCAAAGTGACCATAGTGGACCACCAC
  GCCGCCACAGCCTCCTTCATGAAGCACCTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCTGCCGATT
  GGGCCTGGATTGTGCCCCCCATCTCAGGCAGCCTAACTCCTGTCTTCCATCAAGAGATGGTCAACTATTT
  CCTGTCCCCTGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGAAGTGCAGCAAAGGGGGCAGGCATCACC
  AGGAAGAAGACCTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG
  CGAAGCGTGTGAAGGCAACCATTCTGTATGGCTCTGAGACTGGCCGGGCCCAGAGCTACGCACAGCAGCT
  GGGAAGACTCTTCCGGAAGGCGTTTGATCCCCGGGTCCTGTGCATGGATGAGTATGATGTGGTGTCCCTA
  GAGCACGAGGCACTGGTGTTGGTGGTGACCAGCACATTTGGCAATGGGGATCCTCCGGAGAATGGAGAGA
  GCTTTGCAGCAGCGCTCATGGAAATGTCAGGCCCGTACAACAGCTCCCCTAGGCCTGAGCAGCACAAGAG
  CTACAAAATCCGATTCAACAGTGTCTCCTGCTCAGACCCACTGGTATCCTCTTGGCGGCGCAAGAGGAAG
  GAGTCTAGCAACACAGACAGTGCAGGAGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCC
  GAGCATACCCCCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG
  ACTACTGCAGCTGGGCCAAGGTGATGAGCTCTGTGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCC
  GCCTTCCAGGCTGCCTGTGAAACCTTCTGTGTGGGAGAAGATGCCAAAGCTGCTGCCCGAGATATCTTCA
  GCCCCAAACGCAGCTGGAAGCGCCAGAGGTACCGGCTGAGTACCCAGGCTGAGAGCCTGCAATTACTACC
  AGGGCTGACTCACGTGCACAGGCGGAAGATGTTCCAGGCTACAATCCTCTCTGTGGAAAACCTACAGAGC
  AGCAAATCCACCCGAGCCACGATCCTGGTGCGTCTGGACACCGGAGGCCAGGAGGGACTGCAGTACCAGC
  CAGGGGACCACATAGGTGTGTGCCCACCCAACCGTCCTGGCCTAGTGGAGGCACTGCTGAGCCGAGTGGA
  GGACCCTCCGCCATCCACAGAACCTGTGGCTGTGGAACAACTGGAGAAAGGCAGCCCTGGTGGCCCTCCC
  CCCGGCTGGGTACGGGACCCCCGGCTACCCCCATGTACGCTGCGGCAGGCTCTCACCTACTTCCTGGACA
  TCACTTCCCCGCCTAGTCCTCGCCTCCTTCGACTGCTCAGCACCCTGGCAGAAGAGTCCAGCGAACAGCA
  GGAGCTAGAGGCTCTCAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCAGCTGCCCCACACTG
  CTAGAGGTGCTGGAGCAATTTCCTTCAGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCTTGC
  TCCAGCCCCGGTACTACTCTGTCAGTTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACCATAGC
  TGTGCTGGCTTACAGAACCCAGGATGGGCTGGGCCCTCTGCACTACGGTGTCTGCTCCACGTGGATGAGC
  CAGCTCAAGGCGGGAGATCCAGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC
  CTAACTTGCCCTGCATCCTGGTGGGCCCAGGGACTGGCATTGCACCCTTCCGGGGATTCTGGCAAGACAG
  ACTACACGACATTGAGATCAAAGGGCTACAACCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC
  CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGGGTGTTTGGACAAGTCCTCA
  CCGCCTTTTCCAGGGATCCTGGCAGCCCCAAGACCTACGTGCAAGACCTCCTGAGGACAGAGCTAGCCGC
  GGAGGTTCACCGTGTGCTGTGCCTTGAGCAAGGACATATGTTTGTCTGCGGCGATGTCACTATGGCAACC
  AGCGTCCTGCAAACCGTGCAGAGAATTCTGGCAACAGAGGGCGGCATGGAGCTGGATGAAGCCGGTGACG
  TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA
  GGTGACAAGCCGCATACGCACCCAGAGCTTTTCTTTGCAGGAGCGACAGCTGAGGGGCGCAGTGCCCTGG
  TCCTTTGACCCGCCTGGCCCAGAAATACCTGGTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211798 representing BC052636
  Red=Cloning site Green=Tags(s)
  
  MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPAPPSPTRPAPDHSPPLTRPPDGPRFPRV
  KNWEVGSITYDTLSAQAQQDGPCTSRRCLGSLVFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRS
  GSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYI
  CNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLIRYAGYRQQDGSVRGDPANVEITELCIQHGWTP
  GNGRFDVLPLLLQAPDEPPELFTLPPEMVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAA
  PFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHH
  AATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGAGIT
  RKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSL
  EHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRK
  ESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQA
  AFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPGLTHVHRRKMFQATILSVENLQS
  SKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPP
  PGWVRDPRLPPCTLRQALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFSCPTL
  LEVLEQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTIAVLAYRTQDGLGPLHYGVCSTWMS
  QLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHDIEIKGLQPAPMTLVFGCRCS
  QLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQDLLRTELAAEVHRVLCLEQGHMFVCGDVTMAT
  SVLQTVQRILATEGGMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPW
  SFDPPGPEIPGS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052636
• ORF Size: 3606 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052636.1
• RefSeq Size: 3982
• RefSeq ORF: 3608
• Locus ID: 18127