Npc1 (NM_008720) Mouse Tagged ORF Clone

CAT#: MG211885

Npc1 (GFP-tagged) - Mouse Niemann Pick type C1 (Npc1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Npc1
• Synonyms: A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; spm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211885 representing NM_008720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCGCACCACCCGGCCCTCGGCCTGCTGCTGCTGCTGCTGTGCCCTGCGCAGGTGTTTTCGCAAT
  CCTGTGTTTGGTATGGAGAGTGTGGAATTGCGACTGGAGATAAGAGGTACAACTGTAAATATTCTGGCCC
  ACCAAAACCCCTCCCAAAGGACGGCTATGACTTAGTGCAGGAACTCTGTCCAGGACTCTTCTTTGACAAT
  GTCAGTCTCTGCTGTGACATTCAACAGCTTCAGACGCTGAAGAGTAACCTGCAGCTGCCCCTGCAGTTCC
  TGTCCAGGTGTCCGTCATGTTTTTATAACCTAATGACCCTGTTTTGTGAGCTAACATGTAGCCCACACCA
  GAGTCAGTTTCTGAATGTGACAGCAACTGAGGATTATTTTGATCCTAAGACACAGGAGAATAAAACAAAT
  GTAAAGGAATTAGAGTACTTTGTCGGACAGAGCTTCGCGAATGCGATGTACAATGCCTGCCGTGATGTGG
  AGGCGCCTTCCAGTAACGAGAAGGCCTTAGGACTCTTGTGTGGGAGGGATGCCCGTGCCTGCAATGCCAC
  CAACTGGATTGAGTACATGTTCAATAAAGACAACGGACAAGCGCCATTTACCATCATTCCTGTGTTTTCA
  GATCTTTCAATCCTTGGGATGGAGCCCATGAGAAATGCCACCAAAGGCTGCAATGAGTCTGTAGATGAGG
  TCACGGGGCCATGTAGCTGCCAGGACTGCTCCATCGTCTGCGGCCCCAAGCCCCAGCCCCCACCCCCTCC
  TATGCCCTGGAGGATCTGGGGCTTGGATGCCATGTATGTCATCATGTGGGTCACCTACGTGGCATTTCTG
  TTTGTGTTTTTTGGAGCACTGTTGGCAGTGTGGTGCCACAGAAGGCGGTACTTTGTGTCTGAGTACACTC
  CCATTGACAGTAACATCGCCTTTTCTGTGAATAGCAGTGACAAAGGGGAAGCCTCATGCTGTGACCCACT
  TGGTGCAGCATTTGATGACTGTCTGAGGCGCATGTTCACAAAGTGGGGGGCTTTCTGTGTCCGAAATCCC
  ACCTGCATCATTTTCTTCTCATTGGCCTTCATCACTGTGTGCTCTTCTGGCCTGGTATTTGTCCAGGTCA
  CCACCAATCCTGTAGAGCTCTGGTCAGCCCCTCACAGTCAGGCCCGCTTGGAAAAGGAGTACTTTGACAA
  GCACTTTGGGCCTTTCTTTCGCACGGAGCAGCTTATCATCCAGGCCCCCAACACCAGTGTTCATATCTAC
  GAACCGTACCCCGCAGGAGCCGATGTGCCCTTCGGGCCTCCATTGAACAAAGAGATTCTGCACCAGGTTC
  TGGACTTACAGATCGCCATTGAAAGCATCACCGCATCTTACAACAATGAAACCGTGACACTGCAGGACAT
  CTGTGTGGCCCCCCTCTCTCCATACAACAAGAACTGCACCATTATGAGTGTGTTAAATTACTTCCAGAAC
  AGCCATGCGGTGCTGGACAGCCAAGTAGGCGACGACTTCTATATCTACGCTGATTACCACACACACTTTC
  TGTACTGTGTACGGGCTCCCGCCTCCTTGAATGATACGAGTTTGCTCCACGGTCCTTGCCTGGGTACATT
  TGGAGGACCGGTGTTCCCGTGGCTTGTGTTGGGTGGCTATGATGATCAGAACTACAACAATGCCACCGCG
  CTTGTGATCACCTTCCCCGTGAATAATTACTACAATGACACAGAGAGGCTCCAGAGGGCCTGGGCCTGGG
  AGAAAGAGTTTATTAGTTTTGTGAAAAACTACAAGAATCCAAATCTGACCATTTCTTTCACTGCTGAGCG
  AAGCATCGAAGATGAGCTCAATCGGGAAAGTAACAGTGACGTGTTCACCGTCATCATCAGCTACGTCGTG
  ATGTTTCTGTACATTTCCCTCGCCCTGGGTCACATCCAGAGCTGCAGCAGGCTCCTGGTGGATTCTAAGA
  TCTCGCTGGGCATTGCGGGGATCCTGATCGTGCTAAGCTCGGTGGCCTGCTCTCTGGGCATCTTCAGCTA
  CATGGGGATGCCGCTGACCCTCATCGTCATTGAGGTCATCCCATTCCTGGTGCTGGCTGTCGGGGTGGAC
  AACATCTTCATTCTAGTGCAGACCTACCAGAGAGATGAGCGTCTTCAGGAGGAAACGCTGGATCAGCAGC
  TGGGCAGGATCCTTGGAGAAGTGGCCCCGACCATGTTCCTTTCATCCTTTTCTGAGACCTCAGCATTTTT
  CTTTGGGGCGCTGTCCTCGATGCCAGCTGTGCACACCTTCTCTCTGTTTGCGGGAATGGCCGTCCTCATT
  GACTTCCTCCTTCAGATTACCTGCTTTGTGAGCCTGTTGGGGTTAGATATTAAGAGGCAAGAGAAAAACC
  ATCTGGACATCCTGTGCTGTGTCAGAGGCGCTGACGACGGACAAGGTAGCCACGCCTCCGAAAGCTACCT
  GTTTCGCTTCTTCAAAAACTACTTTGCCCCCCTTCTGCTGAAGGACTGGCTGAGGCCAATTGTGGTAGCG
  GTGTTTGTGGGCGTTCTGTCATTCAGTGTTGCGGTGGTGAACAAAGTAGACATCGGGTTGGATCAGTCTC
  TCTCAATGCCAAACGATTCGTATGTGATTGACTATTTCAAATCACTCGCTCAGTACCTGCACTCGGGCCC
  ACCCGTGTACTTTGTCCTGGAGGAAGGCTATAACTACAGTTCACGCAAAGGGCAGAACATGGTGTGCGGC
  GGCATGGGCTGTGACAATGACTCCCTGGTGCAGCAGATATTTAACGCAGCTGAGCTGGACACCTACACCC
  GAGTAGGCTTCGCCCCCTCGTCCTGGATCGATGACTACTTTGACTGGGTCTCGCCACAGTCGTCCTGCTG
  CAGACTCTACAACGTCACTCACCAGTTCTGCAATGCTTCTGTGATGGACCCAACCTGTGTCCGCTGCAGA
  CCTCTGACTCCAGAGGGTAAACAGAGGCCTCAGGGGAAAGAATTCATGAAATTCCTGCCCATGTTCCTTT
  CTGATAACCCCAACCCCAAGTGCGGCAAAGGGGGACATGCTGCTTACGGTTCAGCTGTTAACATTGTGGG
  AGATGACACTTACATTGGGGCCACTTACTTCATGACCTACCACACCATACTTAAGACCTCCGCTGACTAT
  ACTGATGCCATGAAGAAAGCTCGGCTAATAGCCAGTAACATCACGGAAACCATGCGTTCTAAGGGGAGTG
  ACTACCGCGTATTCCCTTACAGTGTGTTCTACGTCTTCTATGAACAGTACCTGACCATTATTGATGACAC
  CATCTTTAACCTCAGTGTGTCTCTGGGCTCCATATTTCTGGTGACCTTGGTGGTTCTGGGCTGTGAGCTG
  TGGTCTGCGGTCATCATGTGTATCACCATAGCCATGATCCTGGTCAACATGTTCGGTGTCATGTGGCTGT
  GGGGCATCAGTCTGAATGCGGTCTCCTTGGTCAACTTGGTGATGAGCTGCGGCATTTCTGTGGAGTTCTG
  CAGCCATATAACGAGAGCATTCACCATGAGTACCAAAGGAAGCCGAGTGAGCCGGGCGGAAGAGGCACTG
  GCCCACATGGGTAGTTCTGTATTCAGTGGAATCACACTTACGAAATTTGGAGGGATCGTGGTGTTAGCCT
  TTGCCAAATCTCAAATTTTTGAGATATTTTACTTCAGGATGTACTTAGCCATGGTCTTACTCGGAGCCAC
  TCATGGACTAATATTTCTTCCCGTCTTACTCAGTTACATAGGGCCGTCGGTGAATAAAGCTAAAAGACAC
  ACCACTTACGAGCGCTACAGAGGGACAGAGAGAAAACGGCTCCTCAATTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211885 representing NM_008720
  Red=Cloning site Green=Tags(s)
  
  MGAHHPALGLLLLLLCPAQVFSQSCVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLVQELCPGLFFDN
  VSLCCDIQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPKTQENKTN
  VKELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQAPFTIIPVFS
  DLSILGMEPMRNATKGCNESVDEVTGPCSCQDCSIVCGPKPQPPPPPMPWRIWGLDAMYVIMWVTYVAFL
  FVFFGALLAVWCHRRRYFVSEYTPIDSNIAFSVNSSDKGEASCCDPLGAAFDDCLRRMFTKWGAFCVRNP
  TCIIFFSLAFITVCSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNTSVHIY
  EPYPAGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTIMSVLNYFQN
  SHAVLDSQVGDDFYIYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATA
  LVITFPVNNYYNDTERLQRAWAWEKEFISFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVIISYVV
  MFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVD
  NIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLI
  DFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVA
  VFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPPVYFVLEEGYNYSSRKGQNMVCG
  GMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVMDPTCVRCR
  PLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVGDDTYIGATYFMTYHTILKTSADY
  TDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLGCEL
  WSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEAL
  AHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKRH
  TTYERYRGTERKRLLNF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008720
• ORF Size: 3831 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008720.2, NP_032746.2
• RefSeq Size: 5209
• RefSeq ORF: 3834
• Locus ID: 18145
• Gene Summary: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. [UniProtKB/Swiss-Prot Function]