Npc1 (NM_008720) Mouse Untagged Clone

CAT#: MC224120

Npc1 (untagged) - Mouse Niemann Pick type C1 (Npc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Npc1
• Synonyms: A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; spm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224120 representing NM_008720
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCGCACCACCCGGCCCTCGGCCTGCTGCTGCTGCTGCTGTGCCCTGCGCAGGTGTTTTCGCAAT
  CCTGTGTTTGGTATGGAGAGTGTGGAATTGCGACTGGAGATAAGAGGTACAACTGTAAATATTCTGGCCC
  ACCAAAACCCCTCCCAAAGGACGGCTATGACTTAGTGCAGGAACTCTGTCCAGGACTCTTCTTTGACAAT
  GTCAGTCTCTGCTGTGACATTCAACAGCTTCAGACGCTGAAGAGTAACCTGCAGCTGCCCCTGCAGTTCC
  TGTCCAGGTGTCCGTCATGTTTTTATAACCTAATGACCCTGTTTTGTGAGCTAACATGTAGCCCACACCA
  GAGTCAGTTTCTGAATGTGACAGCAACTGAGGATTATTTTGATCCTAAGACACAGGAGAATAAAACAAAT
  GTAAAGGAATTAGAGTACTTTGTCGGACAGAGCTTCGCGAATGCGATGTACAATGCCTGCCGTGATGTGG
  AGGCGCCTTCCAGTAACGAGAAGGCCTTAGGACTCTTGTGTGGGAGGGATGCCCGTGCCTGCAATGCCAC
  CAACTGGATTGAGTACATGTTCAATAAAGACAACGGACAAGCGCCATTTACCATCATTCCTGTGTTTTCA
  GATCTTTCAATCCTTGGGATGGAGCCCATGAGAAATGCCACCAAAGGCTGCAATGAGTCTGTAGATGAGG
  TCACGGGGCCATGTAGCTGCCAGGACTGCTCCATCGTCTGCGGCCCCAAGCCCCAGCCCCCACCCCCTCC
  TATGCCCTGGAGGATCTGGGGCTTGGATGCCATGTATGTCATCATGTGGGTCACCTACGTGGCATTTCTG
  TTTGTGTTTTTTGGAGCACTGTTGGCAGTGTGGTGCCACAGAAGGCGGTACTTTGTGTCTGAGTACACTC
  CCATTGACAGTAACATCGCCTTTTCTGTGAATAGCAGTGACAAAGGGGAAGCCTCATGCTGTGACCCACT
  TGGTGCAGCATTTGATGACTGTCTGAGGCGCATGTTCACAAAGTGGGGGGCTTTCTGTGTCCGAAATCCC
  ACCTGCATCATTTTCTTCTCATTGGCCTTCATCACTGTGTGCTCTTCTGGCCTGGTATTTGTCCAGGTCA
  CCACCAATCCTGTAGAGCTCTGGTCAGCCCCTCACAGTCAGGCCCGCTTGGAAAAGGAGTACTTTGACAA
  GCACTTTGGGCCTTTCTTTCGCACGGAGCAGCTTATCATCCAGGCCCCCAACACCAGTGTTCATATCTAC
  GAACCGTACCCCGCAGGAGCCGATGTGCCCTTCGGGCCTCCATTGAACAAAGAGATTCTGCACCAGGTTC
  TGGACTTACAGATCGCCATTGAAAGCATCACCGCATCTTACAACAATGAAACCGTGACACTGCAGGACAT
  CTGTGTGGCCCCCCTCTCTCCATACAACAAGAACTGCACCATTATGAGTGTGTTAAATTACTTCCAGAAC
  AGCCATGCGGTGCTGGACAGCCAAGTAGGCGACGACTTCTATATCTACGCTGATTACCACACACACTTTC
  TGTACTGTGTACGGGCTCCCGCCTCCTTGAATGATACGAGTTTGCTCCACGGTCCTTGCCTGGGTACATT
  TGGAGGACCGGTGTTCCCGTGGCTTGTGTTGGGTGGCTATGATGATCAGAACTACAACAATGCCACCGCG
  CTTGTGATCACCTTCCCCGTGAATAATTACTACAATGACACAGAGAGGCTCCAGAGGGCCTGGGCCTGGG
  AGAAAGAGTTTATTAGTTTTGTGAAAAACTACAAGAATCCAAATCTGACCATTTCTTTCACTGCTGAGCG
  AAGCATCGAAGATGAGCTCAATCGGGAAAGTAACAGTGACGTGTTCACCGTCATCATCAGCTACGTCGTG
  ATGTTTCTGTACATTTCCCTCGCCCTGGGTCACATCCAGAGCTGCAGCAGGCTCCTGGTGGATTCTAAGA
  TCTCGCTGGGCATTGCGGGGATCCTGATCGTGCTAAGCTCGGTGGCCTGCTCTCTGGGCATCTTCAGCTA
  CATGGGGATGCCGCTGACCCTCATCGTCATTGAGGTCATCCCATTCCTGGTGCTGGCTGTCGGGGTGGAC
  AACATCTTCATTCTAGTGCAGACCTACCAGAGAGATGAGCGTCTTCAGGAGGAAACGCTGGATCAGCAGC
  TGGGCAGGATCCTTGGAGAAGTGGCCCCGACCATGTTCCTTTCATCCTTTTCTGAGACCTCAGCATTTTT
  CTTTGGGGCGCTGTCCTCGATGCCAGCTGTGCACACCTTCTCTCTGTTTGCGGGAATGGCCGTCCTCATT
  GACTTCCTCCTTCAGATTACCTGCTTTGTGAGCCTGTTGGGGTTAGATATTAAGAGGCAAGAGAAAAACC
  ATCTGGACATCCTGTGCTGTGTCAGAGGCGCTGACGACGGACAAGGTAGCCACGCCTCCGAAAGCTACCT
  GTTTCGCTTCTTCAAAAACTACTTTGCCCCCCTTCTGCTGAAGGACTGGCTGAGGCCAATTGTGGTAGCG
  GTGTTTGTGGGCGTTCTGTCATTCAGTGTTGCGGTGGTGAACAAAGTAGACATCGGGTTGGATCAGTCTC
  TCTCAATGCCAAACGATTCGTATGTGATTGACTATTTCAAATCACTCGCTCAGTACCTGCACTCGGGCCC
  ACCCGTGTACTTTGTCCTGGAGGAAGGCTATAACTACAGTTCACGCAAAGGGCAGAACATGGTGTGCGGC
  GGCATGGGCTGTGACAATGACTCCCTGGTGCAGCAGATATTTAACGCAGCTGAGCTGGACACCTACACCC
  GAGTAGGCTTCGCCCCCTCGTCCTGGATCGATGACTACTTTGACTGGGTCTCGCCACAGTCGTCCTGCTG
  CAGACTCTACAACGTCACTCACCAGTTCTGCAATGCTTCTGTGATGGACCCAACCTGTGTCCGCTGCAGA
  CCTCTGACTCCAGAGGGTAAACAGAGGCCTCAGGGGAAAGAATTCATGAAATTCCTGCCCATGTTCCTTT
  CTGATAACCCCAACCCCAAGTGCGGCAAAGGGGGACATGCTGCTTACGGTTCAGCTGTTAACATTGTGGG
  AGATGACACTTACATTGGGGCCACTTACTTCATGACCTACCACACCATACTTAAGACCTCCGCTGACTAT
  ACTGATGCCATGAAGAAAGCTCGGCTAATAGCCAGTAACATCACGGAAACCATGCGTTCTAAGGGGAGTG
  ACTACCGCGTATTCCCTTACAGTGTGTTCTACGTCTTCTATGAACAGTACCTGACCATTATTGATGACAC
  CATCTTTAACCTCAGTGTGTCTCTGGGCTCCATATTTCTGGTGACCTTGGTGGTTCTGGGCTGTGAGCTG
  TGGTCTGCGGTCATCATGTGTATCACCATAGCCATGATCCTGGTCAACATGTTCGGTGTCATGTGGCTGT
  GGGGCATCAGTCTGAATGCGGTCTCCTTGGTCAACTTGGTGATGAGCTGCGGCATTTCTGTGGAGTTCTG
  CAGCCATATAACGAGAGCATTCACCATGAGTACCAAAGGAAGCCGAGTGAGCCGGGCGGAAGAGGCACTG
  GCCCACATGGGTAGTTCTGTATTCAGTGGAATCACACTTACGAAATTTGGAGGGATCGTGGTGTTAGCCT
  TTGCCAAATCTCAAATTTTTGAGATATTTTACTTCAGGATGTACTTAGCCATGGTCTTACTCGGAGCCAC
  TCATGGACTAATATTTCTTCCCGTCTTACTCAGTTACATAGGGCCGTCGGTGAATAAAGCTAAAAGACAC
  ACCACTTACGAGCGCTACAGAGGGACAGAGAGAGAACGGCTCCTCAATTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008720
• ORF Size: 3834 bp
• Insert Size: 3834
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_008720.2, NP_032746.2
• RefSeq Size: 5209
• RefSeq ORF: 3834
• Locus ID: 18145
• Gene Summary: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. [UniProtKB/Swiss-Prot Function]