Wrn (NM_011721) Mouse Tagged ORF Clone

CAT#: MG211966

Wrn (GFP-tagged) - Mouse Werner syndrome homolog (human) (Wrn)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wrn
• Synonyms: AI846146
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211966 representing NM_011721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCACTTCACTACAGCGGAAATTTCCAGAATGGATGTCTATGCAGAGTCAAAGATGTGCTACAG
  AAGAAAAGGCCTGCGTTCAGAAGAGTGTTCTTGAAGATAACCTCCCATTCTTAGAATTCCCTGGATCCAT
  TGTTTACAGTTATGAAGCTAGTGATTGCTCCTTCCTGTCTGAAGACATTAGCATGCGTCTGTCTGATGGC
  GATGTGGTGGGATTTGACATGGAATGGCCGCCCATATACAAGCCAGGGAAAAGAAGCAGAGTCGCAGTGA
  TCCAGTTGTGTGTGTCTGAGAGCAAATGTTACTTGTTTCACATTTCTTCCATGTCAGTTTTCCCCCAGGG
  ATTAAAAATGTTACTAGAAAACAAATCAATTAAGAAGGCAGGGGTTGGGATTGAAGGGGACCAGTGGAAA
  CTTCTGCGTGATTTTGACGTCAAGTTGGAGAGTTTTGTGGAGCTGACGGATGTTGCCAATGAAAAGTTGA
  AGTGCGCAGAGACCTGGAGCCTCAATGGTCTGGTTAAACACGTCTTAGGGAAACAACTTTTGAAAGACAA
  GTCCATCCGCTGCAGCAATTGGAGTAATTTCCCCCTCACTGAGGACCAGAAACTGTATGCAGCCACTGAT
  GCTTATGCTGGTCTTATCATCTATCAAAAATTAGGAAATTTGGGTGATACTGCGCAAGTGTTTGCTCTAA
  ATAAAGCAGAGGAAAACCTACCTCTGGAGATGAAGAAACAGTTGAATTCAATCTCCGAAGAAATGAGGGA
  CCTAGCCAATCGTTTTCCTGTCACTTGCAGAAATTTGGAAACTCTCCAGAGGGTTCCTGTAATATTGAAG
  AGTATTTCAGAAAATCTCTGTTCATTGAGAAAAGTGATCTGTGGTCCTACAAACACTGAGACTAGACTGA
  AGCCGGGCAGTAGTTTTAATTTACTGTCATCAGAGGATTCAGCTGCTGCTGGAGAAAAAGAGAAACAGAT
  TGGAAAACATAGTACTTTTGCTAAAATTAAAGAAGAACCATGGGACCCAGAACTTGACAGTTTAGTGAAG
  CAAGAGGAGGTTGATGTATTTAGAAATCAAGTGAAGCAAGAAAAAGGTGAATCTGAAAATGAAATAGAAG
  ACAATCTGTTGAGAGAAGATATGGAAAGAACTTGTGTGATTCCTAGTATTTCAGAAAATGAACTCCAAGA
  TTTGGAACAGCAAGCTAAAGAAGAAAAATATAATGATGTTTCTCACCAACTTTCTGAGCATTTATCTCCC
  AATGATGATGAGAATGACTCCTCCTATATAATTGAAAGTGATGAAGATTTGGAAATGGAGATGCTGAAGT
  CTTTAGAAAACCTAAATAGTGACGTGGTGGAACCCACTCACTCTACATGGTTGGAAATGGGAACCAATGG
  GCGTCTTCCTCCTGAGGAGGAAGATGGACACGGAAATGAAGCCATCAAAGAGGAGCAGGAAGAAGAGGAC
  CATTTATTGCCGGAACCCAACGCAAAGCAAATTAATTGCCTCAAGACCTATTTCGGACACAGCAGTTTTA
  AACCGGTTCAGTGGAAAGTCATCCATTCTGTATTAGAAGAGAGAAGAGATAATGTTGTTGTCATGGCAAC
  TGGATATGGGAAGAGTCTGTGCTTCCAGTATCCGCCTGTTTATACAGGCAAGATTGGCATTGTCATTTCA
  CCTCTCATTTCCTTAATGGAAGACCAAGTCCTCCAGCTTGAGCTGTCCAATGTTCCAGCCTGTTTACTTG
  GATCTGCACAGTCAAAAAATATTCTAGGAGATGTTAAATTAGGCAAATATAGGGTCATCTACATAACTCC
  AGAGTTCTGTTCTGGTAACTTGGATCTACTCCAGCAACTTGACTCTAGTATTGGCATCACTCTCATTGCT
  GTGGATGAGGCTCACTGCATTTCAGAGTGGGGCCATGATTTCAGAAGTTCATTCAGGATGCTGGGCTCTC
  TTAAAACAGCGCTCCCATTGGTTCCAGTCATTGCACTCTCCGCTACTGCAAGCTCTTCCATCCGGGAAGA
  CATTATAAGCTGCTTAAACCTGAAAGACCCTCAGATCACCTGCACTGGATTTGATCGGCCAAATCTGTAC
  TTAGAAGTTGGACGGAAAACAGGGAACATCCTTCAGGATCTAAAGCCGTTTCTCGTCCGAAAGGCAAGTT
  CTGCCTGGGAATTTGAAGGTCCAACCATCATCTATTGTCCTTCGAGAAAAATGACAGAACAAGTTACTGC
  TGAACTTGGGAAACTGAACTTAGCCTGCAGAACATACCACGCTGGCATGAAAATTAGCGAAAGGAAGGAC
  GTTCATCATAGGTTCCTGAGAGATGAAATTCAGTGTGTTGTAGCTACTGTAGCTTTTGGAATGGGCATTA
  ATAAAGCTGACATTCGCAAAGTTATTCATTATGGTGCGCCTAAGGAAATGGAATCCTATTACCAGGAAAT
  TGGTAGAGCTGGCCGGGATGGACTTCAGAGTTCCTGTCACTTGCTCTGGGCTCCAGCAGACTTTAACACA
  TCCAGGAATCTCCTTATTGAGATTCACGATGAAAAGTTCCGGTTATATAAATTAAAGATGATGGTAAAGA
  TGGAAAAATACCTTCACTCCAGTCAGTGTAGGCGACGAATCATCTTGTCCCATTTTGAGGACAAATGTCT
  GCAGAAGGCCTCCTTGGACATTATGGGAACTGAAAAATGCTGTGATAATTGCAGGCCCAGGCTGAATCAT
  TGCCTTACTGCTAACAACTCAGAGGACGCATCCCAAGACTTTGGGCCACAAGCATTCCAGCTACTGTCTG
  CTGTGGACATCCTGCAGGAGAAATTTGGAATTGGGATTCCGATCTTATTTCTCCGAGGATCTAATTCTCA
  GCGTCTTCCTGATAAATATCGGGGTCACAGGCTCTTTGGTGCTGGAAAGGAGCAAGCAGAAAGTTGGTGG
  AAGACCCTTTCTCACCATCTCATAGCTGAAGGATTCTTGGTAGAAGTTCCCAAGGAAAACAAATATATAA
  AGACATGTTCCCTCACAAAAAAGGGTAGAAAGTGGCTTGGAGAAGCCAGTTCGCAGTCTCCTCCGAGCCT
  TCTCCTTCAAGCTAATGAAGAGATGTTTCCAAGGAAAGTTCTGCTACCAAGTTCTAATCCTGTATCTCCA
  GAAACGACGCAACATTCCTCTAATCAAAACCCAGCTGGATTAACTACCAAGCAGTCTAATTTGGAGAGAA
  CGCATTCTTACAAAGTGCCTGAGAAAGTTTCTTCTGGGACTAACATTCCTAAAAAAAGTGCCGTGATGCC
  GTCACCAGGAACATCTTCCAGCCCCTTAGAACCTGCCATCTCAGCCCAAGAGCTGGACGCTCGGACTGGG
  CTATATGCCAGGCTGGTGGAAGCAAGGCAGAAACACGCTAATAAGATGGATGTACCTCCAGCTATTTTAG
  CAACAAACAAGGTTCTGCTGGACATGGCTAAAATGAGACCGACTACTGTTGAAAACATGAAACAGATCGA
  CGGTGTCTCTGAAGGCAAAGCTGCTCTGTTGGCCCCTCTGTTGGAAGTCATCAAACATTTCTGTCAAGTA
  ACTAGTGTTCAGACAGACCTCCTTTCCAGTGCCAAACCTCACAAGGAACAGGAGAAAAGTCAGGAGATGG
  AAAAGAAAGACTGCTCACTCCCCCAGTCTGTGGCCGTCACATACACTCTATTCCAGGAAAAGAAAATGCC
  CTTACACAGCATAGCTGAGAACAGGCTCCTGCCTCTCACAGCAGCCGGCATGCACTTAGCCCAGGCGGTG
  AAAGCCGGCTACCCCCTGGATATGGAGCGAGCTGGCCTGACCCCAGAGACTTGGAAGATTATTATGGATG
  TCATCCGAAACCCTCCCATCAACTCAGATATGTATAAAGTTAAACTCATCAGAATGTTAGTTCCTGAAAA
  CTTAGACACGTACCTCATCCACATGGCGATTGAGATTCTTCAGAGTGGTTCCGACAGCAGAACCCAGCCT
  CCTTGTGATTCCAGCAGGAAGAGGCGTTTCCCCAGCTCTGCAGAGAGTTGTGAGAGCTGTAAGGAGAGCA
  AAGAGGCGGTCACCGAGACCAAGGCATCATCTTCAGAGTCAAAGAGAAAATTACCCGAGTGGTTTGCCAA
  AGGAAATGTGCCCTCAGCTGATACCGGCAGCTCATCATCAATGGCCAAGACCAAAAAGAAAGGTCTCTTT
  AGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211966 representing NM_011721
  Red=Cloning site Green=Tags(s)
  
  METTSLQRKFPEWMSMQSQRCATEEKACVQKSVLEDNLPFLEFPGSIVYSYEASDCSFLSEDISMRLSDG
  DVVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWK
  LLRDFDVKLESFVELTDVANEKLKCAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD
  AYAGLIIYQKLGNLGDTAQVFALNKAEENLPLEMKKQLNSISEEMRDLANRFPVTCRNLETLQRVPVILK
  SISENLCSLRKVICGPTNTETRLKPGSSFNLLSSEDSAAAGEKEKQIGKHSTFAKIKEEPWDPELDSLVK
  QEEVDVFRNQVKQEKGESENEIEDNLLREDMERTCVIPSISENELQDLEQQAKEEKYNDVSHQLSEHLSP
  NDDENDSSYIIESDEDLEMEMLKSLENLNSDVVEPTHSTWLEMGTNGRLPPEEEDGHGNEAIKEEQEEED
  HLLPEPNAKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYTGKIGIVIS
  PLISLMEDQVLQLELSNVPACLLGSAQSKNILGDVKLGKYRVIYITPEFCSGNLDLLQQLDSSIGITLIA
  VDEAHCISEWGHDFRSSFRMLGSLKTALPLVPVIALSATASSSIREDIISCLNLKDPQITCTGFDRPNLY
  LEVGRKTGNILQDLKPFLVRKASSAWEFEGPTIIYCPSRKMTEQVTAELGKLNLACRTYHAGMKISERKD
  VHHRFLRDEIQCVVATVAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHLLWAPADFNT
  SRNLLIEIHDEKFRLYKLKMMVKMEKYLHSSQCRRRIILSHFEDKCLQKASLDIMGTEKCCDNCRPRLNH
  CLTANNSEDASQDFGPQAFQLLSAVDILQEKFGIGIPILFLRGSNSQRLPDKYRGHRLFGAGKEQAESWW
  KTLSHHLIAEGFLVEVPKENKYIKTCSLTKKGRKWLGEASSQSPPSLLLQANEEMFPRKVLLPSSNPVSP
  ETTQHSSNQNPAGLTTKQSNLERTHSYKVPEKVSSGTNIPKKSAVMPSPGTSSSPLEPAISAQELDARTG
  LYARLVEARQKHANKMDVPPAILATNKVLLDMAKMRPTTVENMKQIDGVSEGKAALLAPLLEVIKHFCQV
  TSVQTDLLSSAKPHKEQEKSQEMEKKDCSLPQSVAVTYTLFQEKKMPLHSIAENRLLPLTAAGMHLAQAV
  KAGYPLDMERAGLTPETWKIIMDVIRNPPINSDMYKVKLIRMLVPENLDTYLIHMAIEILQSGSDSRTQP
  PCDSSRKRRFPSSAESCESCKESKEAVTETKASSSESKRKLPEWFAKGNVPSADTGSSSSMAKTKKKGLF
  S
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011721
• ORF Size: 6380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011721.2
• RefSeq Size: 6476
• RefSeq ORF: 4206
• Locus ID: 22427