Ank3 (NM_170729) Mouse Tagged ORF Clone

CAT#: MG215345

Ank3 (GFP-tagged) - Mouse ankyrin 3 epithelial (Ank3) transcript variant 8, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ank3
• Synonyms: 2900054D09Rik; AI314020; Ank-3; AnkG; Ankyrin-3; Ankyrin-G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215345 representing NM_170729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAGAGCCAAAGGAGAAGCCCGCCAAGCCTGCTCATAGGAAGAGGAAAGGAAAAAAGTCTGATG
  CCAACGCAAGTTACTTAAGAGCAGCTCGGGCAGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGG
  AGTGGACGTCAACATCTGTAACCAGAATGGATTGAATGCACTCCATCTTGCTTCCAAAGAAGGCCATGTG
  GAAGTGGTCTCTGAGCTGCTGCAGAGGGAAGCCAATGTTGATGCCGCCACAAAGAAAGGAAACACGGCCT
  TACACATCGCATCTTTGGCTGGGCAAGCGGAAGTGGTCAAGGTCTTGGTTACGAACGGAGCGAATGTCAA
  CGCACAATCTCAGAATGGCTTCACACCATTGTATATGGCAGCCCAGGAGAACCACCTGGAAGTCGTCAGG
  TTTCTTCTGGACAATGGCGCCAGCCAAAGCCTGGCCACAGAGGACGGCTTCACGCCATTGGCCGTGGCTC
  TGCAACAAGGTCATGACCAAGTCGTGTCCCTCCTGCTCGAGAACGACACGAAGGGAAAAGTGCGCCTCCC
  AGCCCTCCACATCGCAGCCCGGAAAGACGACACCAAGGCAGCAGCTCTGCTCCTGCAGAATGACACAAAC
  GCGGACGTGGAGTCAAAGAGTGGCTTCACCCCGCTCCACATAGCTGCCCACTATGGGAACATCAATGTGG
  CCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGACTTCACCGCACGGAATGACATCACTCCCTTACACGT
  TGCCTCGAAGCGAGGAAATGCAAATATGGTGAAGCTATTGCTGGACCGGGGTGCGAAGATCGATGCCAAG
  ACCAGGGACGGTCTGACTCCGTTGCACTGTGGGGCGAGAAGTGGCCATGAGCAGGTGGTAGAGATGTTGC
  TTGACAGATCCGCCCCCATCCTTTCAAAAACCAAGAATGGATTGTCGCCACTGCACATGGCCACACAAGG
  AGACCATTTAAACTGCGTCCAACTCCTCCTCCAGCACAACGTGCCCGTGGACGACGTCACCAACGACTAC
  CTGACTGCCCTCCATGTGGCTGCCCACTGCGGCCATTACAAAGTTGCCAAGGTTCTTTTGGATAAGAAAG
  CTAGCCCCAATGCCAAAGCCCTGAATGGCTTCACCCCTCTCCATATCGCCTGCAAAAAGAACCGCATCCG
  AGTAATGGAACTCCTTTTGAAGCACGGTGCATCTATTCAAGCCGTAACCGAGTCGGGCCTTACCCCAATC
  CATGTTGCTGCCTTCATGGGACATGTAAATATCGTGTCACAGCTAATGCATCATGGAGCCTCCCCAAACA
  CCACCAATGTGAGAGGAGAGACGGCATTGCATATGGCGGCTCGGTCCGGACAAGCAGAAGTGGTGCGGTA
  TCTGGTCCAAGATGGGGCTCAGGTAGAAGCAAAAGCTAAGGATGACCAGACTCCACTCCACATCTCAGCC
  CGACTTGGGAAAGCTGACATAGTGCAACAACTGTTACAGCAAGGAGCATCCCCCAATGCAGCAACAACTT
  CTGGGTACACCCCCCTTCACCTTGCGGCCAGAGAGGGGCATGAGGATGTAGCTGCGTTCCTCCTGGATCA
  TGGAGCATCTTTATCCATAACAACAAAGAAGGGATTCACCCCTCTGCACGTGGCAGCCAAATACGGAAAG
  CTTGAAGTCGCAAGTCTCCTGCTGCAGAAGAGTGCGTCTCCCGATGCCGCAGGGAAGAGCGGGCTAACTC
  CACTGCATGTAGCAGCGCATTACGATAATCAGAAAGTGGCCCTTCTGCTCTTGGACCAGGGAGCCTCACC
  CCACGCAGCCGCAAAGAATGGCTATACACCACTGCACATCGCGGCCAAGAAGAACCAGATGGACATAGCC
  ACGTCCCTGCTGGAGTACGGTGCTGATGCAAACGCGGTTACCCGGCAAGGGATTGCGTCCGTCCATCTTG
  CGGCACAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTGAGTAGAAACGCGAATGTCAACCTGAGCAA
  TAAGAGCGGTCTCACCCCACTCCACCTGGCTGCTCAAGAAGACCGAGTGAATGTGGCCGAGGTCCTTGTC
  AACCAGGGGGCCCATGTGGATGCTCAGACAAAGATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATG
  GAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGAATGGATA
  CACAGCACTGCACCAGGCTGCTCAGCAGGGCCACACGCATATCATCAATGTCTTGCTTCAGAACAACGCC
  TCCCCCAATGAACTCACTGTGAATGGGAACACAGCTCTGGCCATCGCCCGGCGCCTTGGTTACATCTCGG
  TGGTTGACACACTGAAGGTCGTGACGGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAAT
  GAATGTCCCAGAAACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGGTGAAGATGCCATCACAGGG
  GACACTGACAAGTATCTCGGGCCACAGGACCTTAAGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTT
  ACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTT
  GAACAGAAGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCAC
  CTGACGTTCACGAGGGAGTTTGATTCTGACTCCCTCAGACACTACAGTTGGGCAGCGGACACGTTAGATA
  ATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGG
  CTCCATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACC
  CGCATCACGTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGAT
  TAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCC
  TCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAACTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGG
  AAGGAACATCAGTTTGACAGTAAAAACGAAGACCTCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCG
  ACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTGCAGAATTATCACAAAGGATTTCCCCCAGTATTTTGC
  CGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACC
  GTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCGCCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTC
  AGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCTTGGGAACAAAGCAACATTTAGCCCAATTGTCACGGT
  AGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATCACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAA
  GGCGTGTCCAATGGGTACAAGGGGGATGCCACGCCCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCA
  CCTCACCAGCTCAATGGGAAGACATCACAGGAACAACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTT
  CACAACCAACGTTTCAGCCAGATTCTGGCTGGCGGACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCC
  TCCCAGCTGTACAGAGAGCTGATATGCGTTCCCTACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACG
  ACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTATGACAGACGACAGGGTGGACAAAACCCTGGAGCAGCA
  GGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGACATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGAT
  TGCTATGGAAACCTGGCCCCTCTGACCAAAGGAGGACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAG
  AAAACAGACTGCCATTTTCCATCAAGATCAGAGACACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCT
  GAAGGAGCCAAAGACAACAAAGGGATTACCCCAAACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCA
  CATAAAAAGGCTGAGAAGGCAGACAGACGCCAGAGCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCT
  ACTTGACTGAACCCAGCATGAGTCCGCAGAGTCCTTGTGAGCGGACGGATATCAGGATGGCGATAGTAGC
  CGATCACCTGGGACTTAGTTGGACAGAGCTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAACCAA
  ATACGTGTGGAAAATCCCAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAG
  ACGGAAAGAATGCCACAACTGATGCCTTAACTTCGGTCTTAACGAAGATTAACCGGATAGACATTGTAAC
  TCTGCTGGAAGGACCAATATTTGATTATGGGAATATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAAT
  GTTTTCCATGACCCAGTTGATGGTCACCCTTCCTTTCAAGTGGAGCTGGAGACCCCCATGGGGTTGTACT
  GCACACCACCCAACCCTTTCCAGCAAGATGACCATTTTAGTGATATCTCTAGCATAGAGTCTCCCTTTAG
  GACCCCCAGTAGACTGAGTGACGGGCTGGTGCCTTCCCAGGGAAACATAGAGCATCCAACAGGTGGACCT
  CCAGTGGTAACCGCAGAGGACACTTCTTTAGAAGACAGCAAAATGGACGATTCTGTAACTGTAACAGACC
  CGGCCGACCCACTGGACGTAGATGAGAGCCAGTTGAAGGACCTGTGTCAGAGCGAGTGTGCTCAGTGCTG
  GGCGAGTGTGCCCGGGATCCCAAACGACGGTCGGCAGGCAGAGCCACTGAGACCGCAGACTAGAAAAGTA
  GGCATGAGCTCTGAACAGCAGGAAAAAGGAAAATCTGGTCCTGATGAGGAAGTGACAGAAGACAAGGTCA
  AATCTCTGTTTGAGGACATTCAACTTGAAGAAGTAGAGGCTGAGGAGATGACAGAAGACCAGGGGCAGGC
  TATGCTTAACCGTGTTCAGCGAGCAGAACTGGCAATGTCTTCACTTGCAGGTTGGCAGAACGAGACGCCA
  AGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCGAAGACTAACTGGTGGGTTACTGGACCGTCTGGATGACA
  GCTCTGACCAGGCTCGGGATTCTATTACCTCATACCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGG
  AAACCACACAGCGGAAGTCATTCCAGAAGCAAAGGCAAAACCCTACTTCCCGGAATCCCAAAACGATATA
  GGGAAACAGAGCATCAAGGAGAACCTGAAACCAAAAACACACGGATGTGGTCGCACTGAGGAACCAGTGT
  CGCCCCTCACAGCCTACCAGAAATCTCTGGAAGAAACCAGCAAGCTTGTCATAGAAGACGCACCTAAACC
  CTGTGTGCCTGTCGGCATGAAAAAGATGACCAGGACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAG
  GAAGAAGAGGGGTCCACCAGGTCAGAGCCTAAGCAGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAA
  TCCGGAACGTGGAGAAGAAAACCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215345 representing NM_170729
  Red=Cloning site Green=Tags(s)
  
  MSEEPKEKPAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHV
  EVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVR
  FLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTN
  ADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK
  TRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY
  LTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPI
  HVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISA
  RLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGK
  LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIA
  TSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLV
  NQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNA
  SPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEGEDAITG
  DTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMMIEELLVPSKEQH
  LTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPT
  RITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETW
  KEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTT
  VPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGE
  GVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLA
  SQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKDIEVLEGKPIYVD
  CYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPA
  HKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQ
  IRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENN
  VFHDPVDGHPSFQVELETPMGLYCTPPNPFQQDDHFSDISSIESPFRTPSRLSDGLVPSQGNIEHPTGGP
  PVVTAEDTSLEDSKMDDSVTVTDPADPLDVDESQLKDLCQSECAQCWASVPGIPNDGRQAEPLRPQTRKV
  GMSSEQQEKGKSGPDEEVTEDKVKSLFEDIQLEEVEAEEMTEDQGQAMLNRVQRAELAMSSLAGWQNETP
  SGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIEANGNHTAEVIPEAKAKPYFPESQNDI
  GKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTRTTADGKARLNLQ
  EEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170729
• ORF Size: 5766 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_170729.2, NP_733925.2
• RefSeq Size: 9908
• RefSeq ORF: 5769
• Locus ID: 11735
• Gene Summary: This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]