Ank3 (NM_170687) Mouse Tagged ORF Clone

CAT#: MG215427

Ank3 (GFP-tagged) - Mouse ankyrin 3 epithelial (Ank3) transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ank3
• Synonyms: 2900054D09Rik; AI314020; Ank-3; AnkG; Ankyrin-3; Ankyrin-G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215427 representing NM_170687
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGCCACACAGTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACAGGACCTTA
  AGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAG
  CCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATG
  ATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCACCTGACGTTCACGAGGGAGTTTGATTCTGACTCCC
  TCAGACACTACAGTTGGGCAGCGGACACGTTAGATAATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGG
  GTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGCGAGGAAGCCGCCACCACGGGATGCGG
  ATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACCCGCATCACGTGCCGCCTGGTAAAGAGACATAAAC
  TGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGG
  GGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCCTCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAA
  CTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGGAAGGAACATCAGTTTGACAGTAAAAACGAAGACC
  TCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCGACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTG
  CAGAATTATCACAAAGGATTTCCCCCAGTATTTTGCCGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAG
  ATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACCGTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCG
  CCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTCAGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCT
  TGGGAACAAAGCAACATTTAGCCCAATTGTCACGGTAGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATC
  ACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAAGGCGTGTCCAATGGGTACAAGGGGGATGCCACGC
  CCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCACCTCACCAGCTCAATGGGAAGACATCACAGGAAC
  AACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTTCACAACCAACGTTTCAGCCAGATTCTGGCTGGCG
  GACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCCTCCCAGCTGTACAGAGAGCTGATATGCGTTCCCT
  ACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACGACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTAT
  GACAGACGACAGGGTGGACAAAACCCTGGAGCAGCAGGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGAC
  ATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGATTGCTATGGAAACCTGGCCCCTCTGACCAAAGGAG
  GACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAGAAAACAGACTGCCATTTTCCATCAAGATCAGAGA
  CACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCTGAAGGAGCCAAAGACAACAAAGGGATTACCCCAA
  ACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCACATAAAAAGGCTGAGAAGGCAGACAGACGCCAGA
  GCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAACCCAGCATGAGTCCGCAGAGTCC
  TTGTGAGCGGACGGATATCAGGATGGCGATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAGCTGGCA
  AGGGAACTGAATTTTTCAGTGGATGAAATCAACCAAATACGTGTGGAAAATCCCAATTCTTTAATTTCTC
  AGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAGACGGAAAGAATGCCACAACTGATGCCTTAACTTC
  GGTCTTAACGAAGATTAACCGGATAGACATTGTAACTCTGCTGGAAGGACCAATATTTGATTATGGGAAT
  ATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCCATGACCCAGTTGATGGTCACCCTTCCT
  TTCAAGTGGAGCTGGAGACCCCCATGGGGTTGTACTGCACACCACCCAACCCTTTCCAGCAAGATGACCA
  TTTTAGTGATATCTCTAGCATAGAGTCTCCCTTTAGGACCCCCAGTAGACTGAGTGACGGGCTGGTGCCT
  TCCCAGGGAAACATAGAGCATCCAACAGGTGGACCTCCAGTGGTAACCGCAGAGGACACTTCTTTAGAAG
  ACAGCAAAATGGACGATTCTGTAACTGTAACAGACCCGGCCGACCCACTGGACGTAGATGAGAGCCAGTT
  GAAGGACCTGTGTCAGAGCGAGTGTGCTCAGTGCTGGGCGAGTGTGCCCGGGATCCCAAACGACGGTCGG
  CAGGCAGAGCCACTGAGACCGCAGACTAGAAAAGTAGGCATGAGCTCTGAACAGCAGGAAAAAGGAAAAT
  CTGGTCCTGATGAGGAAGTGACAGAAGACAAGGTCAAATCTCTGTTTGAGGACATTCAACTTGAAGAAGT
  AGAGGCTGAGGAGATGACAGAAGACCAGGGGCAGGCTATGCTTAACCGTGTTCAGCGAGCAGAACTGGCA
  ATGTCTTCACTTGCAGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCGAA
  GACTAACTGGTGGGTTACTGGACCGTCTGGATGACAGCTCTGACCAGGCTCGGGATTCTATTACCTCATA
  CCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGGAAACCACACAGCGGAAGTCATTCCAGAAGCAAAG
  GCAAAACCCTACTTCCCGGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACCTGAAACCAA
  AAACACACGGATGTGGTCGCACTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTGGAAGA
  AACCAGCAAGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCAGG
  ACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAAGAGGGGTCCACCAGGTCAGAGCCTAAGC
  AGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAATCCGGAACGTGGAGAAGAAAACCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215427 representing NM_170687
  Red=Cloning site Green=Tags(s)
  
  MALPHSEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMM
  IEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMR
  IIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERE
  LIVLRSENGETWKEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQ
  IGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPI
  TMTIPVPPPSGEGVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA
  DCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKD
  IEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQ
  TAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADHLGLSWTELA
  RELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGN
  ISGTRSFADENNVFHDPVDGHPSFQVELETPMGLYCTPPNPFQQDDHFSDISSIESPFRTPSRLSDGLVP
  SQGNIEHPTGGPPVVTAEDTSLEDSKMDDSVTVTDPADPLDVDESQLKDLCQSECAQCWASVPGIPNDGR
  QAEPLRPQTRKVGMSSEQQEKGKSGPDEEVTEDKVKSLFEDIQLEEVEAEEMTEDQGQAMLNRVQRAELA
  MSSLAGWQNETPSGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIEANGNHTAEVIPEAK
  AKPYFPESQNDIGKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTR
  TTADGKARLNLQEEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170687
• ORF Size: 3282 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_170687.3, NP_733788.2
• RefSeq Size: 7554
• RefSeq ORF: 3285
• Locus ID: 11735
• Gene Summary: This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]