Tpd52 (NM_001025263) Mouse Tagged ORF Clone

CAT#: MG215470

Tpd52 (GFP-tagged) - Mouse tumor protein D52 (Tpd52) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpd52
• Synonyms: AI043038; mD52
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215470 representing NM_001025263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCGGCGAGCAAGGTCTGCTGAAGACAGAGCCGGTGGCCGAGGAAGGAGAGGATGCTGTTACCA
  TGCTCAGTGCTCCAGAGGCGCTGACGGAAGAGGAGCAAGAGGAGCTGAGGCGGGAGCTTACTAAGGTGGA
  AGAAGAAATCCAGACTCTGTCCCAAGTATTGGCCGCAAAAGAGAAGCATCTCGCCGAGCTCAAGCGGAAG
  CTCGGCATCTCCTCGCTTCAGGAGTTCAAGCAGAACATTGCCAAAGGGTGGCAAGACGTGACGGCAACCA
  ATGCATACAAGAAGACCTCTGAAACTCTATCGCAAGCTGGGCAGAAGGCCTCCGCTGCATTTTCATCGGT
  TGGCTCAGTCATCACCAAAAAGCTGGAAGACGTGAATATCCGCTCCATTCAGCATTCGATTAGCATGCCT
  GCTATGAGAAACTCCCCAACTTTCAAGTCATTTGAAGAAAAAGTTGAAAATTTAAAGTCTAAAGTAGGAG
  GAGCCAAGCCTGCTGGCGGCGATTTTGGAGAAGTCCTGAATTCCACAGCCAACGCTACCAGTACCATGAC
  CACAGAGCCTCCTCCAGAACAGATGACAGAGAGCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215470 representing NM_001025263
  Red=Cloning site Green=Tags(s)
  
  MDRGEQGLLKTEPVAEEGEDAVTMLSAPEALTEEEQEELRRELTKVEEEIQTLSQVLAAKEKHLAELKRK
  LGISSLQEFKQNIAKGWQDVTATNAYKKTSETLSQAGQKASAAFSSVGSVITKKLEDVNIRSIQHSISMP
  AMRNSPTFKSFEEKVENLKSKVGGAKPAGGDFGEVLNSTANATSTMTTEPPPEQMTESP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025263
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025263.1, NP_001020434.1
• RefSeq Size: 2308 bp
• RefSeq ORF: 600 bp
• Locus ID: 21985
• Cytogenetics: 3 A1