Tle2 (NM_019725) Mouse Tagged ORF Clone

CAT#: MG215597

Tle2 (GFP-tagged) - Mouse transducin-like enhancer of split 2 homolog of Drosophila E(spl) (Tle2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tle2
• Synonyms: Grg2; mKIAA4188
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215597 representing NM_019725
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCCTCAGGGAAGGCACCCGACCCCGCTGCAGTCTGGCCAGCCTTTCAAGTTCTCAGTACTGGAAA
  TCTGTGACCGGATCAAAGAGGAATTCCAGTTTCTTCAAGCTCAGTACCACAGCCTCAAGCTAGAATGTGA
  GAAGCTGGCCAGCGAGAAGACAGAAATGCAAAGGCATTATGTGATGGCTGCACCCCATCAGTGTCCCCAG
  GGTGGCACCAGCTATCCACACTGGCCAAGACTGTCTCCTTTGCAGTACTACGAGATGTCCTACGGACTCA
  ACATTGAGATGCATAAACAGGCTGAGATTGTGAAACGCCTCAGTGCGATCTGTGCCCAGATGGTCCCGTT
  CCTCACTCAGGAGCATCAGCAGCAGGTGCTCCAGGCTGTGGACCGAGCCAAGCAGGTGACCGTGGGGGAA
  CTGAACAGCCTCCTGGGGCAGCAGAATCAGCTCCAGCCGCTGTCCCACGCACCCCCCGTGCCTCTCACCC
  CGCGCCCAGCCGGCCTGGTGGGTGCCGGGGCCACTGGGCTGCTGGCCCTATCTGGGGCACTGGCTGCGCA
  GGCCCAGCTGGTGGCTGCCGTAAAGGAAGACCGTGTGGGTGTGGACGCCGAGGGGTCCAGAGTGGACAGA
  GCTGCCAGCAGGAGTTCTTCCCCGTCTCCCCCTGAGAGTCTGGTGGAAGAGGACCATCCCAGCAGCCGAG
  GCGGCAGTGGGAAACAGCAGAGAGCTGAAGACAAGGATCTGTCAGGGCCTTATGACAGTGAGGAAGACAA
  GAGTGACTATAACCTGGTAGTGGATGAGGACCAACCGTCAGAGCCCCCCAGCCCTGTGACCACCCCTTGT
  GGGAAGGCGCCCCTCTGCATTCCTGCCCGCAGGGACCTCACAGACAGTCCAGCCTCCTTGGCCTCCAGTT
  TGGGCTCACCACTCCCCAGAAGCAAAGACATAGCCCTGAACGATCTTCCCACAGGCACTCCTGCCTCCAG
  GTCATGTGGTACCTCTCCACCCCAGGACTCGTCCACCCCGGGGCCCAGCTCAGCCAGTCACCTCTGCCAG
  CTGGCGGCTCAGCCGGCAGCACCCACAGACAGCATCGCCCTGAGGAGTCCCCTGACCTTGTCCAGCCCTT
  TCACCTCATCCTTCAGCCTGGGCTCCCACAGCACCCTCAATGGGGACCTCTCCATGCCTGGCTCCTATGT
  CGGCCTCCACCTGTCCCCCCAGGTCAGCAGCTCTGTCGTGTATGGACGCTCACCTCTGCAGATGGCATTT
  GAATCGCACCCCCATCTCCGAGGCTCGTCTGTCTCCTTGCCTGGCATCCCTGTGGCTAAGCCGGCTTACT
  CCTTCCACGTGTCTGCGGATGGCCAGATGCAGCCTGTGCCCTTCCCGTCTGATGCTCTGGTAGGCACAGG
  CATCCCTCGCCACGCAAGGCAGCTACACACGCTGGCCCACGGTGAGGTGGTGTGTGCCGTCACCATCAGC
  AGCTCCACACAGCACGTGTACACAGGCGGCAAAGGATGCGTGAAGGTGTGGGACGTGGGCCAGCCGGGTA
  GCAAGACCCCTGTGGCACAGCTGGATTGCCTGAACCGAGACAACTACATCCGCTCCTGCAAGCTGCTGCC
  CGACGGGCAGAGCCTGATTGTAGGTGGCGAGGCCAGTACCCTGTCCATTTGGGACCTGGCAGCACCCACA
  CCACGGATCAAAGCAGAGCTGACTTCGTCTGCCCCGGCCTGCTATGCTCTGGCCGTCAGTCCGGACGCCA
  AGGTCTGCTTCTCCTGCTGCAGCGACGGTAACATCGTGGTCTGGGACCTGCAGAACCAGGCCATGGTCAG
  ACAGTTCCAGGGCCACACGGACGGGGCCAGCTGCATCGACATATCAGACTACGGGACCCGGCTGTGGACT
  GGGGGCCTGGACAACACTGTGCGCTGCTGGGACCTGCGCGAGGGCCGCCAGCTTCAGCAGCATGACTTCA
  GTTCCCAGATTTTCTCATTGGGCCACTGTCCCAATCAGGACTGGTTGGCTGTGGGGATGGAGAGCAGCCA
  CGTGGAGGTCCTGCATGTGCGCAAGCCTGAGAAGTACCAGCTCCGCCTCCATGAGAGCTGCGTGCTGTCA
  CTCAAGTTCGCTTCCTGTGGACGCTGGTTTGTGAGCACAGGCAAGGACAACCTGCTCAATGCCTGGAGGA
  CACCCTATGGGGCCAGCATTTTTCAGTCCAAAGAATCATCTTCTGTACTGAGCTGCGACATCTCCAGGAA
  TAATAAGTACATCGTGACAGGCTCAGGGGACAAGAAGGCCACTGTGTATGAGGTGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215597 representing NM_019725
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPTPLQSGQPFKFSVLEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMAAPHQCPQ
  GGTSYPHWPRLSPLQYYEMSYGLNIEMHKQAEIVKRLSAICAQMVPFLTQEHQQQVLQAVDRAKQVTVGE
  LNSLLGQQNQLQPLSHAPPVPLTPRPAGLVGAGATGLLALSGALAAQAQLVAAVKEDRVGVDAEGSRVDR
  AASRSSSPSPPESLVEEDHPSSRGGSGKQQRAEDKDLSGPYDSEEDKSDYNLVVDEDQPSEPPSPVTTPC
  GKAPLCIPARRDLTDSPASLASSLGSPLPRSKDIALNDLPTGTPASRSCGTSPPQDSSTPGPSSASHLCQ
  LAAQPAAPTDSIALRSPLTLSSPFTSSFSLGSHSTLNGDLSMPGSYVGLHLSPQVSSSVVYGRSPLQMAF
  ESHPHLRGSSVSLPGIPVAKPAYSFHVSADGQMQPVPFPSDALVGTGIPRHARQLHTLAHGEVVCAVTIS
  SSTQHVYTGGKGCVKVWDVGQPGSKTPVAQLDCLNRDNYIRSCKLLPDGQSLIVGGEASTLSIWDLAAPT
  PRIKAELTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQAMVRQFQGHTDGASCIDISDYGTRLWT
  GGLDNTVRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSHVEVLHVRKPEKYQLRLHESCVLS
  LKFASCGRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019725
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019725.2, NP_062699.1
• RefSeq Size: 2749
• RefSeq ORF: 2304
• Locus ID: 21886