Diaph1 (NM_007858) Mouse Tagged ORF Clone

CAT#: MG215994

Diap1 (GFP-tagged) - Mouse diaphanous homolog 1 (Drosophila) (Diap1), (10ug). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Diaph1
• Synonyms: D18Wsu154e; Dia1; Diap1; Drf1; p140mDia
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215994 representing NM_007858, codon optimized.
  Due to the complexity of NM_007858, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCATCCGGAGGAGGACTTGGACCCGGTCGGGGAACACGCGACAAGAAGAAAGGTCGGTCCCCTG
  ACGAGCTTCCTGCTACGGGAGGTGACGGTGGTAAGCATAAAAAATTCCTCGAGAGGTTCACTTCAATGCG
  GATTAAGAAAGAAAAGGAAAAGCCCAACTCCGCTCACCGAAACAGTTCTGCCAGTTACGGAGACGACCCC
  ACCGCCCAAAGCCTGCAGGATATTTCCGACGAGCAAGTGCTTGTGCTTTTCGAGCAGATGCTGGTTGACA
  TGAACCTTAATGAAGAGAAGCAGCAGCCCCTGAGAGAGAAAGACATCGTTATTAAGAGAGAAATGGTGAG
  TCAGTACCTGCACACCAGCAAAGCTGGGATGAACCAGAAGGAGAGCTCTAGATCCGCTATGATGTACATT
  CAAGAACTGAGATCTGGCCTGCGCGATATGCATCTCCTGTCTTGTCTGGAGAGCCTGAGAGTGAGCCTGA
  ACAACAACCCAGTAAGTTGGGTCCAAACATTCGGGGCAGAAGGTTTGGCTAGCCTGCTGGACATTCTCAA
  ACGGCTGCACGACGAAAAGGAGGAAACATCCGGGAATTATGATTCCCGCAACCAGCACGAGATCATAAGG
  TGTCTGAAGGCGTTCATGAACAACAAGTTCGGCATAAAGACCATGCTCGAAACCGAGGAGGGCATTCTTT
  TGCTGGTCCGCGCCATGGATCCAGCCGTGCCTAACATGATGATTGATGCTGCGAAACTTCTCTCTGCCCT
  GTGTATCCTGCCTCAGCCTGAGGATATGAACGAAAGGGTGCTCGAAGCTATGACTGAACGGGCGGAAATG
  GACGAGGTCGAGAGGTTCCAGCCCCTTCTGGATGGGTTGAAGTCTGGAACTTCTATCGCACTCAAGGTCG
  GTTGTCTCCAACTGATCAACGCGCTGATTACACCCGCCGAGGAGCTGGATTTCCGCGTGCACATAAGATC
  AGAGCTGATGAGGCTCGGCCTGCATCAGGTACTCCAAGAGCTGCGCGAGATCGAAAACGAAGACATGAAG
  GTGCAGCTCTGTGTGTTCGATGAACAGGGCGACGAGGACTTTTTTGATCTCAAGGGACGCCTGGACGACA
  TCCGCATGGAAATGGATGATTTTGGAGAAGTGTTCCAGATCATCCTGAACACAGTTAAAGATTCTAAAGC
  CGAACCACATTTTTTGAGTATTCTGCAGCATCTGCTCCTGGTGAGGAACGACTACGAAGCACGACCCCAA
  TACTATAAGCTCATTGAAGAATGCGTTTCACAGATTGTCCTGCATAAGAATGGGACCGATCCGGATTTCA
  AGTGTAGGCACCTTCAGATCGATATCGAGCGGCTCGTCGACCAAATGATTGATAAGACCAAGGTGGAAAA
  GAGTGAGGCTAAGGCTACCGAACTGGAGAAGAAGCTTGATAGTGAACTCACTGCCAGGCACGAGCTGCAG
  GTCGAGATGAAGAAGATGGAAAATGACTTTGAACAAAAACTGCAGGACTTGCAGGGGGAGAAGGATGCCC
  TCGACTCAGAAAAGCAACAGATTACCGCTCAGAAACAAGATCTGGAAGCTGAGGTTTCCAAGCTTACGGG
  AGAAGTCGCAAAGCTGAGCAAGGAGCTTGAAGATGCCAAGAACGAGATGGCGAGCCTGTCAGCGGTGGTG
  GTCGCTCCTTCCGTGTCTTCTAGCGCAGCCGTGCCCCCTGCCCCCCCGCTTCCGGGAGATTCTGGGACCG
  TGATACCGCCACCCCCACCCCCCCCACCGCTGCCTGGCGGGGTCGTGCCCCCTTCACCACCACTCCCTCC
  TGGTACATGCATCCCACCTCCCCCTCCCCTTCCTGGCGGTGCGTGTATTCCCCCGCCACCTCAGTTGCCC
  GGTAGTGCTGCCATCCCCCCACCTCCGCCACTCCCCGGGGTCGCAAGCATTCCCCCTCCGCCTCCTCTCC
  CAGGCGCGACCGCAATTCCGCCTCCTCCTCCCCTGCCCGGTGCAACCGCAATCCCACCTCCCCCCCCACT
  TCCTGGCGGTACCGGCATCCCGCCCCCTCCACCTCCTTTGCCCGGCAGTGTCGGAGTGCCTCCACCTCCG
  CCGCTGCCCGGAGGACCCGGGCTCCCTCCTCCGCCTCCGCCCTTTCCAGGGGCCCCAGGCATTCCTCCTC
  CCCCTCCCGGGATGGGAGTCCCCCCGCCTCCTCCATTCGGTTTCGGAGTGCCAGCAGCCCCCGTCCTGCC
  GTTTGGCCTCACCCCCAAAAAAGTTTATAAGCCTGAGGTGCAGCTCAGAAGGCCAAATTGGTCTAAGTTT
  GTGGCGGAAGATCTTAGCCAGGACTGCTTCTGGACAAAGGTGAAGGAAGACCGATTTGAGAATAACGAAC
  TTTTCGCTAAACTCACCCTGGCATTCTCAGCACAGACCAAGACTTCTAAAGCAAAAAAGGATCAGGAGGG
  GGGCGAGGAAAAAAAAAGTGTTCAAAAGAAGAAAGTTAAAGAGCTGAAAGTCTTGGATAGCAAAACTGCC
  CAGAACCTCTCTATCTTCCTCGGGTCATTCCGCATGCCTTACCAGGAGATCAAAAATGTCATTCTTGAGG
  TGAATGAGGCAGTGCTGACGGAGAGCATGATCCAAAATCTTATCAAGCAGATGCCCGAGCCGGAGCAGCT
  CAAGATGCTCTCTGAGCTGAAGGAAGAATACGACGACTTGGCCGAGTCTGAGCAATTCGGTGTGGTAATG
  GGGACCGTACCTCGCCTGCGACCCCGACTTAACGCGATCCTGTTTAAACTCCAGTTTTCAGAGCAAGTTG
  AGAATATCAAACCTGAGATCGTGAGTGTGACTGCAGCTTGCGAGGAACTCAGGAAGTCCGAAAACTTCTC
  CAGCTTGCTGGAGCTCACCCTGCTTGTAGGAAACTATATGAATGCCGGAAGCCGGAACGCGGGCGCCTTT
  GGTTTTAATATCTCATTCCTCTGTAAACTGAGGGACACAAAGTCCGCAGACCAGAAAATGACACTCCTCC
  ACTTCCTCGCCGAACTGTGCGAAAACGACCATCCCGAGGTTCTCAAATTCCCCGACGAACTCGCCCACGT
  CGAGAAGGCAAGCAGAGTCTCAGCTGAGAATCTGCAAAAGTCCCTCGACCAGATGAAAAAGCAGATAGCT
  GATGTGGAGCGCGACGTGCAGAATTTCCCCGCTGCTACCGATGAGAAGGACAAGTTTGTGGAAAAGATGA
  CCTCCTTCGTCAAGGACGCCCAGGAGCAATACAATAAGTTGAGGATGATGCACAGCAACATGGAAACCCT
  GTATAAGGAACTCGGCGATTATTTTGTGTTCGACCCCAAGAAGCTGAGTGTCGAGGAGTTTTTTATGGAC
  CTCCATAATTTTAGAAATATGTTTTTGCAAGCCGTCAAGGAGAATCAGAAACGCAGGGAAACCGAAGAGA
  AAATGAGACGAGCCAAGCTCGCAAAGGAGAAGGCAGAAAAGGAACGCCTTGAGAAGCAGCAGAAAAGGGA
  ACAGCTGATTGATATGAACGCTGAAGGCGACGAAACCGGAGTGATGGATTCCCTGTTGGAGGCCCTCCAA
  AGCGGCGCCGCATTCCGGCGAAAGAGGGGCCCCAGACAGGTAAACCGCAAGGCTGGCTGCGCTGTGACTT
  CTCTGCTTGCCAGCGAGTTGACCAAAGACGATGCCATGGCTCCCGGACCTGTGAAAGTACCCAAAAAGTC
  TGAGGGCGTGCCGACGATTCTGGAAGAAGCCAAGGAGCTGGTTGGGAGAGCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215994 representing NM_007858
  Red=Cloning site Green=Tags(s)
  
  MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDP
  TAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYI
  QELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIR
  CLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEM
  DEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMK
  VQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQ
  YYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQ
  VEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVV
  VAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLP
  GSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPP
  PLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKF
  VAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTA
  QNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVM
  GTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAF
  GFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIA
  DVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMD
  LHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQ
  SGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007858
• ORF Size: 3765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007858.2, NP_031884.1
• RefSeq Size: 5666
• RefSeq ORF: 3768
• Locus ID: 13367
• Gene Summary: This gene encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2016]