Diaph1 (NM_007858) Mouse Tagged ORF Clone
CAT#: MG215994
- TrueORF®
Diap1 (GFP-tagged) - Mouse diaphanous homolog 1 (Drosophila) (Diap1), (10ug). Note: ORF is codon optimized
"NM_007858" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Diaph1 |
Synonyms | D18Wsu154e; Dia1; Diap1; Drf1; p140mDia |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215994 representing NM_007858, codon optimized.
Due to the complexity of NM_007858, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCATCCGGAGGAGGACTTGGACCCGGTCGGGGAACACGCGACAAGAAGAAAGGTCGGTCCCCTG ACGAGCTTCCTGCTACGGGAGGTGACGGTGGTAAGCATAAAAAATTCCTCGAGAGGTTCACTTCAATGCG GATTAAGAAAGAAAAGGAAAAGCCCAACTCCGCTCACCGAAACAGTTCTGCCAGTTACGGAGACGACCCC ACCGCCCAAAGCCTGCAGGATATTTCCGACGAGCAAGTGCTTGTGCTTTTCGAGCAGATGCTGGTTGACA TGAACCTTAATGAAGAGAAGCAGCAGCCCCTGAGAGAGAAAGACATCGTTATTAAGAGAGAAATGGTGAG TCAGTACCTGCACACCAGCAAAGCTGGGATGAACCAGAAGGAGAGCTCTAGATCCGCTATGATGTACATT CAAGAACTGAGATCTGGCCTGCGCGATATGCATCTCCTGTCTTGTCTGGAGAGCCTGAGAGTGAGCCTGA ACAACAACCCAGTAAGTTGGGTCCAAACATTCGGGGCAGAAGGTTTGGCTAGCCTGCTGGACATTCTCAA ACGGCTGCACGACGAAAAGGAGGAAACATCCGGGAATTATGATTCCCGCAACCAGCACGAGATCATAAGG TGTCTGAAGGCGTTCATGAACAACAAGTTCGGCATAAAGACCATGCTCGAAACCGAGGAGGGCATTCTTT TGCTGGTCCGCGCCATGGATCCAGCCGTGCCTAACATGATGATTGATGCTGCGAAACTTCTCTCTGCCCT GTGTATCCTGCCTCAGCCTGAGGATATGAACGAAAGGGTGCTCGAAGCTATGACTGAACGGGCGGAAATG GACGAGGTCGAGAGGTTCCAGCCCCTTCTGGATGGGTTGAAGTCTGGAACTTCTATCGCACTCAAGGTCG GTTGTCTCCAACTGATCAACGCGCTGATTACACCCGCCGAGGAGCTGGATTTCCGCGTGCACATAAGATC AGAGCTGATGAGGCTCGGCCTGCATCAGGTACTCCAAGAGCTGCGCGAGATCGAAAACGAAGACATGAAG GTGCAGCTCTGTGTGTTCGATGAACAGGGCGACGAGGACTTTTTTGATCTCAAGGGACGCCTGGACGACA TCCGCATGGAAATGGATGATTTTGGAGAAGTGTTCCAGATCATCCTGAACACAGTTAAAGATTCTAAAGC CGAACCACATTTTTTGAGTATTCTGCAGCATCTGCTCCTGGTGAGGAACGACTACGAAGCACGACCCCAA TACTATAAGCTCATTGAAGAATGCGTTTCACAGATTGTCCTGCATAAGAATGGGACCGATCCGGATTTCA AGTGTAGGCACCTTCAGATCGATATCGAGCGGCTCGTCGACCAAATGATTGATAAGACCAAGGTGGAAAA GAGTGAGGCTAAGGCTACCGAACTGGAGAAGAAGCTTGATAGTGAACTCACTGCCAGGCACGAGCTGCAG GTCGAGATGAAGAAGATGGAAAATGACTTTGAACAAAAACTGCAGGACTTGCAGGGGGAGAAGGATGCCC TCGACTCAGAAAAGCAACAGATTACCGCTCAGAAACAAGATCTGGAAGCTGAGGTTTCCAAGCTTACGGG AGAAGTCGCAAAGCTGAGCAAGGAGCTTGAAGATGCCAAGAACGAGATGGCGAGCCTGTCAGCGGTGGTG GTCGCTCCTTCCGTGTCTTCTAGCGCAGCCGTGCCCCCTGCCCCCCCGCTTCCGGGAGATTCTGGGACCG TGATACCGCCACCCCCACCCCCCCCACCGCTGCCTGGCGGGGTCGTGCCCCCTTCACCACCACTCCCTCC TGGTACATGCATCCCACCTCCCCCTCCCCTTCCTGGCGGTGCGTGTATTCCCCCGCCACCTCAGTTGCCC GGTAGTGCTGCCATCCCCCCACCTCCGCCACTCCCCGGGGTCGCAAGCATTCCCCCTCCGCCTCCTCTCC CAGGCGCGACCGCAATTCCGCCTCCTCCTCCCCTGCCCGGTGCAACCGCAATCCCACCTCCCCCCCCACT TCCTGGCGGTACCGGCATCCCGCCCCCTCCACCTCCTTTGCCCGGCAGTGTCGGAGTGCCTCCACCTCCG CCGCTGCCCGGAGGACCCGGGCTCCCTCCTCCGCCTCCGCCCTTTCCAGGGGCCCCAGGCATTCCTCCTC CCCCTCCCGGGATGGGAGTCCCCCCGCCTCCTCCATTCGGTTTCGGAGTGCCAGCAGCCCCCGTCCTGCC GTTTGGCCTCACCCCCAAAAAAGTTTATAAGCCTGAGGTGCAGCTCAGAAGGCCAAATTGGTCTAAGTTT GTGGCGGAAGATCTTAGCCAGGACTGCTTCTGGACAAAGGTGAAGGAAGACCGATTTGAGAATAACGAAC TTTTCGCTAAACTCACCCTGGCATTCTCAGCACAGACCAAGACTTCTAAAGCAAAAAAGGATCAGGAGGG GGGCGAGGAAAAAAAAAGTGTTCAAAAGAAGAAAGTTAAAGAGCTGAAAGTCTTGGATAGCAAAACTGCC CAGAACCTCTCTATCTTCCTCGGGTCATTCCGCATGCCTTACCAGGAGATCAAAAATGTCATTCTTGAGG TGAATGAGGCAGTGCTGACGGAGAGCATGATCCAAAATCTTATCAAGCAGATGCCCGAGCCGGAGCAGCT CAAGATGCTCTCTGAGCTGAAGGAAGAATACGACGACTTGGCCGAGTCTGAGCAATTCGGTGTGGTAATG GGGACCGTACCTCGCCTGCGACCCCGACTTAACGCGATCCTGTTTAAACTCCAGTTTTCAGAGCAAGTTG AGAATATCAAACCTGAGATCGTGAGTGTGACTGCAGCTTGCGAGGAACTCAGGAAGTCCGAAAACTTCTC CAGCTTGCTGGAGCTCACCCTGCTTGTAGGAAACTATATGAATGCCGGAAGCCGGAACGCGGGCGCCTTT GGTTTTAATATCTCATTCCTCTGTAAACTGAGGGACACAAAGTCCGCAGACCAGAAAATGACACTCCTCC ACTTCCTCGCCGAACTGTGCGAAAACGACCATCCCGAGGTTCTCAAATTCCCCGACGAACTCGCCCACGT CGAGAAGGCAAGCAGAGTCTCAGCTGAGAATCTGCAAAAGTCCCTCGACCAGATGAAAAAGCAGATAGCT GATGTGGAGCGCGACGTGCAGAATTTCCCCGCTGCTACCGATGAGAAGGACAAGTTTGTGGAAAAGATGA CCTCCTTCGTCAAGGACGCCCAGGAGCAATACAATAAGTTGAGGATGATGCACAGCAACATGGAAACCCT GTATAAGGAACTCGGCGATTATTTTGTGTTCGACCCCAAGAAGCTGAGTGTCGAGGAGTTTTTTATGGAC CTCCATAATTTTAGAAATATGTTTTTGCAAGCCGTCAAGGAGAATCAGAAACGCAGGGAAACCGAAGAGA AAATGAGACGAGCCAAGCTCGCAAAGGAGAAGGCAGAAAAGGAACGCCTTGAGAAGCAGCAGAAAAGGGA ACAGCTGATTGATATGAACGCTGAAGGCGACGAAACCGGAGTGATGGATTCCCTGTTGGAGGCCCTCCAA AGCGGCGCCGCATTCCGGCGAAAGAGGGGCCCCAGACAGGTAAACCGCAAGGCTGGCTGCGCTGTGACTT CTCTGCTTGCCAGCGAGTTGACCAAAGACGATGCCATGGCTCCCGGACCTGTGAAAGTACCCAAAAAGTC TGAGGGCGTGCCGACGATTCTGGAAGAAGCCAAGGAGCTGGTTGGGAGAGCCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215994 representing NM_007858
Red=Cloning site Green=Tags(s) MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDP TAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYI QELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIR CLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEM DEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMK VQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQ YYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQ VEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVV VAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLP GSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPP PLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKF VAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTA QNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVM GTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAF GFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIA DVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMD LHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQ SGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007858 |
ORF Size | 3765 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_007858.2, NP_031884.1 |
RefSeq Size | 5666 |
RefSeq ORF | 3768 |
Locus ID | 13367 |
Gene Summary | This gene encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2016] |
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