Pfkfb2 (NM_001162415) Mouse Tagged ORF Clone

CAT#: MG216027

Pfkfb2 (GFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-26-biphosphatase 2 (Pfkfb2) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pfkfb2
• Synonyms: 4930568D07Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216027 representing NM_001162415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGAATAGTACATTTTCCCCAGAAGACTGCAACAGCAGCTATAAACCCCACGCCTCAAATCTGC
  GAAGGGCAGGGAAGACATGCTCATGGGCTTCCTATATGACCAACTCCCCAACACTCATTGTTATGATTGG
  CTTGCCAGCCCGGGGTAAAACTTATGTGTCTAAGAAACTAACACGCTACCTCAACTGGATTGGAGTACCC
  ACCAAAGTGTTTAATCTTGGGGTATATCGACGTGAAGCAGTCAAGTCCTATCAGTCTTATGATTTCTTTC
  GACATGACAATGAAGAGGCTATGAAGATCCGCAAACAGTGTGCTCTGGTGGCACTGGAAGATGTTAAAGC
  CTATTTTACTGAAGAGAGTGGGCAGATTGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGGGAC
  ATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTTTTTGTAGAATCTGTCTGTGATGATCCTG
  ATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGTCGAGCCCTGACTACCCCGAAAGGAATAGGGAGAA
  TGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGACAAC
  TATGATAAGGATCTTTCTTTCATAAAGGTGATAAATGTAGGCCAGAGATTCCTGGTCAACAGAGTTCAGG
  ACTACATCCAGAGTAAGATTGTCTACTACCTGATGAATATCCACGTCCATCCTCGCACCATCTACCTTTG
  CCGGCACGGCGAGAGCGAGTTCAACCTTTTGGGGAAGATTGGGGGTGACTCTGGCCTCTCCGTGCGAGGA
  AAGCAGTTTGCTCATGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTTAAAGTGTGGACGA
  GCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAGTGGAAGATCCTCAA
  TGAGATTGATGCTGGCGTGTGTGAGGAGATGACATATTCAGAGATTGAGCAACGGTACCCAGAGGAATTT
  GCACTCCGAGATCAAGAGAAGTATCTGTACCGATACCCTGGTGGGGAGTCCTACCAGGACCTGGTGCAGC
  GGCTGGAGCCTGTCATCATGGAGCTAGAGCGTCAAGGCAACATCCTCGTTATCTCTCACCAGGCTGTCAT
  GCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTACCATACTTGAGGTGCCCCCTGCAC
  ATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACTCTGAATGTGGATGCTG
  TAGACACACATCGTGACAAGCCAACTGAAGTGGAGAATTTGCTGGCTGAGCATAGACACCCCTCAATGGC
  ATCCCTCACTTTGCTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216027 representing NM_001162415
  Red=Cloning site Green=Tags(s)
  
  MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVP
  TKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRD
  MILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDN
  YDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRG
  KQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEF
  ALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGADELPYLRCPLH
  IIFKLTPVAYGCKVETITLNVDAVDTHRDKPTEVENLLAEHRHPSMASLTLLS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162415
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001162415.1, NP_001155887.1
• RefSeq Size: 3689
• RefSeq ORF: 1422
• Locus ID: 18640
• Gene Summary: Synthesis and degradation of fructose 2,6-bisphosphate. [UniProtKB/Swiss-Prot Function]