Pum1 (NM_030722) Mouse Tagged ORF Clone

CAT#: MG216598

Pum1 (GFP-tagged) - Mouse pumilio 1 (Drosophila) (Pum1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pum1
• Synonyms: AA517475; mKIAA0099; Pumm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216598 representing NM_030722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAAC
  ATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCA
  GCCACAGCCAGCTGCAAATCAGGCTCTTGCAGCTGGAACACACTCCAGCCCTGTCCCAGGATCCATAGGA
  GTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG
  GGGGAGGAGGAAGTGGTGGAGGCGGCTATAATACTAGCAAACATCGATGGCCTACAGGGGATAACATTCA
  TGCAGAACACCAGGTACGCTCTATGGATGAATTGAATCATGATTTTCAAGCACTTGCTCTGGAAGGGAGA
  GCTATGGGCGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAAGATGGACCAA
  AAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCAGATCATTCAGTTTCCCAGCC
  AATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCA
  CGGTCGGAGAGTGGGGGACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGACTCCT
  GTCTAAGAAAAGGAGGATTTGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAA
  CAAGGGTACGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGAGATGTGATGGACAAGACCAAT
  GGTTTGCCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGA
  ACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAG
  CACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATG
  GAACATGTGGGCATGGAGCCTCTGCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCTGCAGCAG
  CAACTGTGGGACTTTTTGACTACAATTCTCAACAACAGCTATTCCAAAGACCCAATGCACTTGCTGTTCA
  GCAGTTGACAGCTGCCCAGCAGCAGCAGTATGCACTTGCAGCAGCCCACCAGCCTCACATCGGTTTAGCT
  CCCGCTGCGTTTGTCCCCAACCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTG
  GATTGGCTGCAGCAGCGACACTCGGCCCAGCTGTGGTCCCTCACCAATATTATGGAGTCACGCCATGGGG
  TGTCTACCCTGCCAGTCTCTTCCAGCAGCAGGCAGCTGCAGCTGCAGCAGCTACAAACTCTGCTACTCAG
  CAGAGCGCTCCGCAGGCCCAGCAGGGACAGCAGCAGGTTCTTCGTGGAGGAGCTAGCCAACGTCCTTTGA
  CCCCAAATCAGAACCAGCAGGGACAACAAACAGATCCCCTTGTAGCAGCTGCAGCAGTGAATTCTGCTCT
  TGCCTTTGGACAAGGATTGGCTGCAGGTATGCCAGGTTATCCAGTCTTGGCACCTGCTGCTTACTATGAC
  CAAACTGGTGCCCTTGTGGTGAATGCAGGGGCAAGAAATGGCCTCGGAGCTCCAGTCCGGCTTGTTGCTC
  CAGCCCCAGTCATCATTAGCTCCTCAGCTGCACAAGCAGCTGTTGCAGCGGCTGCAGCGTCAGCAAACGG
  AGCAGCTGGTGGTCTTGCCGGAACTACAAATGGACCATTTCGACCTTTAGGCACCCAGCAGCCCCAGCCC
  CAGCCTCAGCAGCAGCCCAGTAACAATCTGGCTTCCAGCTCTTTCTATGGCAACAACTCTCTGAGCAGTA
  ACTCCCAGAGCAGCTCCCTCTTCTCTCAGGGCTCTGCACAGCCTGCCAACACGTCCTTGGGATTCGGAAG
  CAGCAGTTCCCTTGGTGCCACCCTGGGATCGGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAAC
  ACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCT
  TGGCCCCCATTGGACACAGTTTTTATAGCAGCCTTAGCTATTCCTCCTCTCCTGGACCCGTGGGCATGCC
  TCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCA
  AGCTTAAACCTGGGAGGACTCACAAACGGCAGTGGAAGATACATCTCTGCTGCTCCCGGTGCCGAAGCCA
  AGTACCGCAGTGCAAGCAGTGCCTCCAGCCTTTTCAGCCCCAGCAGCACCCTTTTTTCTTCCTCTCGTTT
  GCGATATGGAATGTCTGATGTTATGCCCTCTGGCAGGAGCAGACTTCTGGAAGATTTTCGAAACAACCGG
  TACCCTAATTTACAACTGCGGGAGATTGCTGGGCATATAATGGAATTTTCTCAAGACCAGCATGGCTCTA
  GATTCATTCAGCTGAAACTAGAGCGTGCCACAGCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCA
  GGCTGCGTACCAACTTATGGTGGATGTGTTTGGAAATTACGTCATTCAGAAGTTTTTTGAATTTGGCAGT
  CATGAACAGAAGCTGGCTCTGGCTGAACGGATTCGAGGCCATGTCCTATCATTGGCACTGCAGATGTACG
  GCTGCCGAGTCATCCAGAAAGCTTTGGAGTTTATTCCCTCAGACCAGCAGAACGAGATGGTGCGGGAGCT
  AGATGGCCATGTCCTGAAGTGTGTGAAAGACCAGAACGGCAACCATGTGGTTCAGAAGTGCATTGAGTGT
  GTACAGCCCCAGTCTTTGCAGTTTATCATCGATGCATTCAAGGGGCAGGTGTTTGCTTTGTCTACGCACC
  CGTACGGCTGTAGGGTGATCCAGAGGATCCTAGAGCACTGTCTCCCTGACCAGACCCTCCCCATCCTGGA
  GGAGCTTCACCAGCACACAGAGCAGCTTGTGCAGGATCAGTATGGAAATTATGTAATCCAACATGTACTG
  GAGCATGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAGATCCGAGGCAATGTACTTGTATTGAGTC
  AGCACAAGTTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACAGAGCGTGCTGTGCT
  CATTGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGATCAGTAT
  GCTAACTATGTGGTCCAGAAGATGATCGATGTGGCTGAGCCGGGTCAGCGGAAGATTGTCATGCACAAGA
  TCCGGCCACACATTGCGACGCTTCGAAAGTACACCTATGGCAAGCACATCCTGGCCAAGCTCGAGAAGTA
  CTACATGAAGAATGGCGTGGACTTGGGGCCCATTTGTGGTCCCCCTAATGGTATCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216598 representing NM_030722
  Red=Cloning site Green=Tags(s)
  
  MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIG
  VAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNTSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGR
  AMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSP
  RSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTN
  GLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPM
  EHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLA
  PAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSATQ
  QSAPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYD
  QTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQP
  QPQQQPSNNLASSSFYGNNSLSSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSN
  TGSGSRRDSLTGSSDLYKRTSSSLAPIGHSFYSSLSYSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSS
  SLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNR
  YPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATAAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGS
  HEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQNEMVRELDGHVLKCVKDQNGNHVVQKCIEC
  VQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVL
  EHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQY
  ANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030722
• ORF Size: 3567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030722.2, NP_109647.2
• RefSeq Size: 5370
• RefSeq ORF: 3570
• Locus ID: 80912