Cep250 (NM_177217) Mouse Tagged ORF Clone

CAT#: MG217227

Cep250 (GFP-tagged) - Mouse centrosomal protein 250 (Cep250) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cep250
• Synonyms: AW490617; B230210E21Rik; Cep2; Inmp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217227 representing NM_177217
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAGGGAGCCCCGGGTTGAACATGAAGCCCCAGTCCTTGCAGCTGGTGCTGGAGGGGCAGGTGC
  TGGCGCTGCAGCAGCAGATGGCCGAGAACCAGGCTGCCTCCTGGCGGAAACTGAAGAACTCCCAAGAGGC
  CCAGAAGAGACAAGCAACCCTCGTGAGGAAGCTGCAGGCCAAGGTTTTACAGTACCGGAGCTGGTGTCAA
  GACCTGGAGAAGCGACTAGAAGCCACTGGGGGGCTGATCCCTCAGCGCTGGGAGAGTGTAGAAGAGCCCA
  ACCTGGAACAGTTGCTGATCCGATTGGAGGAGGAGCAGCAGAGGTGTGAGAGTCTAGTGGAGGTGAACAC
  CGAGCTTCGGCTGCACATGGAGAAAGCCGATGTGGTGAATAAAGCCTTGCAGGAGGACGTGGAAAAACTG
  ACAGTGGACTGGAGTCGGGCCCGAGATGAGTTAGTGAGGAAGGAGAGCCAGTGGCGGATGGAGCAGGAGT
  TCTTCAAGGGCTATTTAAGAGGTGAACACGGTCGTCTTCTCAATCTATGGCGAGAGGTGGTAACCTTCCG
  ACGTCACTTCCTGAAAATGAAGTCAGCTACTGACAGAGACCTGACAGAGCTAAAGGCTGAACACGCAAGG
  CTTTCAGGGTCCCTGCTGACCTGCTGTCTGCGCCTGACCCTGAGAGCACAGTCTCGGGAGTCCAGTGGAT
  CTGGGAGAACGGAGGAGAGTGAGCCAGCCCGGCTGCTGCTGCTCGTCGCTAAGACCCAGGCGCTGGAGAA
  GGAAGCCCATGAGAAGAGCCAGGAGTTAATGCAGCTCAAGAGTCACGGGGATCTAGAGAAGGCAGAGCTG
  CAGGACCGAGTGACGGAACTCTCTGCTCTGCTAACCCAGTCTCAGAAGCAAAATGAAGATTATGAGAAGA
  TGGTAAAGGCTCTAAGAGAAACAATGGAGATCCTGGAGACAAACCATGCAGAGTTAATGGAGCATGAGGC
  ATCTCTCAGTAGGAATGCTCAAGAGGAAAAGTTGTCTCTGCAGCAGGTGATCAAGGCTATAACCCAGGCA
  CTGGCCTCAGTGGAAGAAGAAGACACTGTGACCCAGTCCTCAGGCCATGAGGACTTGCTGCAGTCAGACT
  GTAATGGCCTTTCCCAGTTTGATCCCCAGGACCCAGACAGGGCTCTTACCCTGGTGCAGTCAGTGCTGAC
  TCGGAGACAGCAGGCTGTGCAGGACCTCAGGCAGCAGCTTTCAGGCTGCCAGGAGGCTATGAGCTTCTTG
  CAGCAGCAGCGCGATCAGTGGGAGGAAGAGGGCAGGGCCCTGAGGGAAAGGCTGCAGAAGCTCACTGGGG
  AGCGGGATGCTCTGGCAGGCCAGACTGTGGACCTTCAGGGAGAGGTGGACTCTCTCAGCAGGGAGCGGGA
  GCTGCTTCAGAAGGCCAGAGGAGAGCTGCAGCAGCAACTGGAAGTGCTGGAGCAGGAAGCGTGGCGACTG
  CGGAGGATGAACATGGAGCTGCAGCTGCAGGGGGACTCAGCTCAAGGGGAGAGGCTGGAGCAGCAGGAGG
  AGCTGCACTTGGCTGTCCGGGAGAGGGAGCGCCTTCAGGAGACGCTGGTGGGCCTGGAAGCCAAGCAGTC
  AGAATCACTAAGTGAGCTGCTCACTCTTCGAGAAGCCCTGGAGTCAAGTCGCCTAGAAGGGGAGTTACTG
  AAGCAGGAGCGAGTGGAAGTGGCTGCAGCCTTGGCCAGGGCAGAACAGTCCATTGTGGAGCTGTCAGGTT
  CTGAGAACAGCCTGAAGGCAGAGGTAGCTGATCTTCGAGCTGCAGCTGTCAAGCTTGGGGCCTTAAATGA
  GGCTTTGGCTTTAGATAAAGTTGGACTGAACCAGCAGCTTCTCCAGTTGGAGCAGGAGAACCAGTCTCTG
  TGCAGCAGAGTGGAGGCCGCAGAGCAGCTGAGAAGCGCTTTGCGGGTGGACCTGGCGGAGGCGGAAAGGC
  GCAGGGAAGCCCTGTGGGAGAAGAAAACTCAGCTGGAGACTCAGCTGCAGAAGGCAGAGGAAGCGGGGGC
  TGAGCTACAGGCAGAGCTGAGGGGCACCCGAGAAGAAAAGGAAGAGCTTAAAGATAAATTAAGTGAGGCA
  CATCACCAGCAAGAAACAGCTACAGCTCACCTAGAACAGCTGCATCAGGACGCAGAAAGACAGGAAGAAA
  CACTTGCTAGGGCAGTCCAGGAGAAGGAAGCCCTAGTACGGGAGAGGGCAGCTCTTGAGGTGCGCCTGCA
  GGCTGTGGAGCGGGACCGACAGGACCTCACTGAACACGTCCTGGGGCTCAGGTCAGCCAAGGAGCAACTG
  GAGAGCAATCTGTTTGAGGCCCAACAGCAAAATTCTGTGATACAGGTCACCAAGGGGCAGCTGGAGGTCC
  AGATTCAAACTATTATCCAAGCCAAGGAAGTAATTCAAGGGGAAGTGAAATGCCTGAAGCTGGAACTGGA
  TGCTGAGAGGACCCGGGCAGAGCAGGAGCGGGATGCAGTAGCCAGGCAGCTAGCCCAAGCTGAGCAGGAG
  GGGCAAGCATCTCTGGAGCGACAGAAGGTGGCCCACGAGGAGGAGGTGAACAGGCTGCAAGAGAAATGGG
  AGAAAGAGCGCTCCTGGCTGCAGCAGGAGCTGGATAAGACCCTGGAAACCCTCGAGAGGGAGAGAGCAGA
  GCTGGAGACAAAGCTGAGAGAGCAGCAGACAGAAATGGAAGCCATCCGTACACAGAGGGAAGAGGAACGA
  AGCCAGGCTGACAGCGCCCTGTATCAAATGCAGCTAGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGA
  CACTGCTGAGGACCCAGAAGGAGCTGGCAGATGCTAGTCAACAGCTGGAAAGGCTGAGACAGGACATGAA
  GATTCAGAAGTTAAAGGAACAGGAGACCACTGGCATGCTGCAGGCCCAGCTTCAGGAAACACAGCAGGAG
  CTGAAGGAGGCAGCCCAGCAGCACAGGGACGACCTTGCTGCCTTCCAGAAGGACAAGCTGGACCTGCAGA
  AGCAGGTAGAGGACCTAATGTCTCAGCTGGTAGCCCACGATGACTCCCAGAGGCTGGTGAAAGAAGAGAT
  TGAAGAGAAGGTGAAAGTGGCTCAAGAATGCAGCCGGATTCAGAAGGAACTGGAGAAAGAGAACGCCAGC
  CTGGCTCTGTCGCTGGTGGAAAAGGAAAAACGACTTCTCATTTTACAAGAAGCTTACTCTGTAAGACAGC
  AGGAGCTGAGCTCCTTGCGCCAGGACATACAGGAGGCCCAGGAAGGGCAAAGAGAGCTGGGTGTACAGGT
  GGAACTATTGAGGCAGGAGGTGAAGGAAAAGGAGGCTGACTTTGTGGCCCGGGAAGCACAGCTGCTGGAG
  GAGTTGGAGGCCTCACGGGTTGCAGAGCAGCAACTTCGAGCGTCTTTGTGGGCCCAGGAAGCCAAGGCAA
  CCCAGCTGCAGCTGCAGCTGCGCAGCACTGAGAGCCAGCTAGAGGCATTGGTTGCAGAACAACAGCCGGA
  GAACCAGGCACAGGCACAGCTGGCTAGCCTCTGCTCTGTTCTGCAGCAGGCCCTGGGGTCTGCATGTGAG
  AGTAGGCCTGAGCTGAGGGGTGGAGGCGACTCTGCTCCTACCTTATGGGGCCCTGATCCAGATCAGAATG
  GAGCTTCCAGACTCTTTAAGAGAGGGTCGCTCCCAACGGCACTGTCACCGGAGGCTGTGGCACTAGCTCT
  CCAGAAGCTCCATCAGGACGTGTGGAAGGCTCGGCAGGCCCGGGATGACCTAAGGGACCAGGTCCAGAAG
  CTGGTGCAGCGCCTAACTGACACGGAGGCCCAGAAGAGCCAGGTTCACTCGGAGCTGCAGGACCTGCAGA
  GACAGCTCTCTCAGAGCCAGGAAGAGAAATCCAAGTGGGAAGGGAGGCAGAACTCCCTGGAGTCTGAGCT
  GAGGGACTTGCACGAGACCGCGGCATCCTTACAGAGTCGCCTCCGGCAGGCAGAACTGCAAAAAATGGAA
  GCTCAGAATGATCGAGAGTTGCTTCAGGCATCCAAGGAGAAGCTGTCAGCCCAGGTTGAACATCTACAAG
  CATGTGTTGCAGAAGCCCAGGCTCAGGCAGGTGCAGCTCCTGTCCTGGAAGAAGATCTGCGAACTGCTCG
  CTCTGCACTGAAACTGAAAAACGAGGAGCTAGAGAGTGAACGGGAGAGAGCCCAGGCTTTACAAGAACAG
  GGTGAGCTGAAGGTGGCTCAGGGGAAAGCTTTGCAGGAGAACCTAGCCCTGCTGGCACAGACCCTGTCTA
  ACAGAGAGCGGGAGGTCGAGACGTTGCAGGCTGAGGTTCAGGAACTGGAGAAGCAGCGGGAGATGCAGAA
  GGCTGCTCTGGAGCTGCTCTCCCTCGACCTGAAGAAGCGCAGTCGGGAGGTAGACCTGCAACAAGAACAG
  ATTCAGGAGCTGGAGCAGTGCAGGTCAGTCTTAGAGCACTTGCCCATGGCCGTCCAAGAGCGAGAGCAGA
  AACTGAGTGTGCAGCGGGACCAGATCAGGGAACTGGAGAACGATCGGGAGGCCCAGAGGAGTGTCCTGGA
  ACACCAGCTTCTGGACCTGGAGCAGAAGGCTCAAGTGATTGAGTCCCAGAGGGGACAGATTCAGGATCTG
  AAAAAGCAACTGGGGACACTGGAATGCCTGGCCCTGGAACTGGAGGAAAGCCATCACAAAGTGGAGAGCC
  AACAGAAGATGATCACAGAACTAGAGGGCCAGAGGGAAATACAAAGGGTGGCTCTAACCCACCTCACACT
  GGACCTGGAAGAAAGGAGCCAGGAGCTGCAGGCACAAAGCAGCCAGCTCCATGAGCTGGAGAACCATAGC
  ACCCATCTGGCAAAAGAGCTGCAGGAGAGGGACCAGGAGGTGACGTCCCAACGCCAGCAGATCGATGAGC
  TGCAGAAGCAGCAGGAACAGCTGGCTCAGGCCCTGGAGAGGAAAGGCCAGGAGCTGGTGCTGCAGAAGGA
  AAGGATTCAGGTTCTGGAAGATCAGAGGACGCTGCAGACCAAGATCTTAGAGGAGGACCTAGAACAGATC
  AAGCATTCCTTGAGAGAACGCAGCCAGGAGCTGGCCTCTCAGCGGCAGCTGGTGCATGAGCGTGCCGACG
  ATGGAAAGAGCCCGAGTAAAGGCCAGCGAGGGAGCCTGGAGCACTTGAAGCTGATCCTGCGTGATAAGGA
  GAAGGAGGTAGAGTGCCAGCAGGAGCGCATCCAGGAACTTCAGGGCCACATGGGCCAGCTGGAACAGCAG
  CTGCAGGGTCTGCACAGGAAGGTGGGGGAGACTAGCCTGCTCCTGACCCATCGAGAGCAGGAGACAGCAA
  CCCTGCAGCAGCATCTCCAGGAAGCCAAGGAACAAGGGGAACTGAGAGAGCAGGTACTTCAGGGTCAGCT
  GGAGGAGGCCCAGAGAGATCTGGCCCAGAGGGACCATGAACTAGAGACCCTCCGGCAGGAAAAACAGCAG
  ACCCAGGACCAGGAGGAAAGCATGAAGCTAAAGACAAGTGCTCTGCAGGCCGCTCTGGAGCAGGCCCATG
  CGACACTGAAGGAGCGTCAGGGGGAGCTGGAAGAACACAGGGAACAGGTGCGAAGGCTCCAGGAAGAACT
  AGAAGTGGAGGGACGGCAGGTACGGGCCCTGGAGGAGGTGTTGGGGGACCTAAGGGCTGAGTCTCGGGAG
  CACGAGAAGGCTGTGCTGGCCCTTCAACAACGGTGTGCTGAGCAGGCCCAGGAACATGAGGCAGAAGCCA
  GGACCCTGCAGGACAGCTGGCTGCAGGCCCAGGCCACTCTCACAGAACAAGAACAAGAGCTGGCGGCACT
  GCGAGCGGAAAACCAGTATTCCCGCCGACAGGAAGAGGCTGCAGTTAGCCAGGCAGAGGCGCTGCAGGAG
  GCCCTCAGCAAAGCCCAGGCTGCCCTGCAGGAGAAAGAGCAGAGTCTGCTTGAACAGGCTGAGTTGAGCC
  ACACCCTGGAGGCCAGCACTGCCGCCTTGCAAGCTACCCTGGACACCTGCCAGGCACGTGCCCGGCAGCT
  GGAGGAGGCCCTGAGGATTCGGGAAGGTGAGATCCAGGCCCAGGCTCTCCAACACCACGAGGTCACGCAG
  CACCTCCAGCAGGAACTTTGCCAGAAGGAAGAAGAGCTGAGGCAGCTACTAGAGAAGGCTGGGGCCCGGA
  GGAGTCAAGAAAATGGAATCCAAGAGAAGCAAAGTCTGGAGCAAGAGAGACAAGAGGAAACAAGGAGGCT
  CCTTGAGAGCCTAAAGGAGCTGCAGCTGACTGTAGCCCAAAGGGAAGAAGAGATTCTGATGCTGAGGGAG
  GCCTCGTCCCCAAGCCACAGAGCCCTCCCAGCCGAGAAGCCAGCTCTACAACCGCTCCCAGCACAACAGG
  AGTTAGAGCGGCTGCAGACTGCCCTAAGGCAAACAGAGGCCAGGGAGATTGAATGGAGGGAGAAGGCTCA
  GGACCTGGCTCTGTCCCTGGCCCAAAGCAAGGCCAGCATCAGCAGTCTGCAGGAGATAACCATGTTCCTA
  CAAGCCTCTGTTCTAGAGCGGGAATCAGAACAACAAAGACTACAGGAGGAGTTGGTACTCAGCAGACAAG
  CTCTGGAAGAGCAGCAGTCAGGTGGCCCACACTCAACCAGCAGAGCAGACCAGGGACCAAAAGTGGGACA
  GGAAAGCCAGCCTGGAGAGGTAGAGACTGAGCCTAGTCCCGGAGTGGAGGAGAAGGAACGGTTGACACAG
  AGGCTTGAACGTCTGCAGCAAGCGGTGGCAGAGCTGGAAGTTGACCGAAGCAAGCTGCAGTGCCACAATG
  CGCAGCTGCGGACCGCCTTGGAGCAGGTGGAACGAGAACGGAGGAAGCTGAAGAGGGATTCCGTGCGTGC
  ATCCCGGGCAGGATCACTGGAGGCCAGAGAAACTATGACTTCATCCCCCACACAGCAGGATGGGAGAGGC
  AGTCAGAGGGGTTCCTCGGACAGCGTGCTTGTGGTTGAGCTTCAGAGAGAGGTAGCCCTGCTGCGAGCCC
  AGCTGGCCTTGGAGCGAAAGCAGAGGCAGGACTACATTGCACGGTCCGTGCAGACCAGCCGTGAGCTAGC
  AGGCCTGCATCACAGCCTGTCTCACTCCCTCCTCACCGTGGCCCAGGCCCCTGAGGCCACTGTTCTGGAG
  GCTGAGACCCGAAAGCTGGATGAGTCTCTGAATCAGAGTCTGACATCCCCAGGGCCATGTTTGCTACATC
  CCAGTCTGGACACTACTCAAAATACCCACAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217227 representing NM_177217
  Red=Cloning site Green=Tags(s)
  
  METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQ
  DLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKL
  TVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHAR
  LSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAEL
  QDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQA
  LASVEEEDTVTQSSGHEDLLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFL
  QQQRDQWEEEGRALRERLQKLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRL
  RRMNMELQLQGDSAQGERLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELL
  KQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVGLNQQLLQLEQENQSL
  CSRVEAAEQLRSALRVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEA
  HHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQL
  ESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQERDAVARQLAQAEQE
  GQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRTQREEER
  SQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQE
  LKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENAS
  LALSLVEKEKRLLILQEAYSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLE
  ELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACE
  SRPELRGGGDSAPTLWGPDPDQNGASRLFKRGSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQK
  LVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKME
  AQNDRELLQASKEKLSAQVEHLQACVAEAQAQAGAAPVLEEDLRTARSALKLKNEELESERERAQALQEQ
  GELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQ
  IQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDL
  KKQLGTLECLALELEESHHKVESQQKMITELEGQREIQRVALTHLTLDLEERSQELQAQSSQLHELENHS
  THLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQI
  KHSLRERSQELASQRQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQ
  LQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQ
  TQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESRE
  HEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQE
  ALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQARARQLEEALRIREGEIQAQALQHHEVTQ
  HLQQELCQKEEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLRE
  ASSPSHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFL
  QASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQESQPGEVETEPSPGVEEKERLTQ
  RLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRG
  SQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLE
  AETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177217
• ORF Size: 7242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177217.3, NP_796191.2
• RefSeq Size: 7914
• RefSeq ORF: 7245
• Locus ID: 16328