Cdk10 (NM_194446) Mouse Tagged ORF Clone

CAT#: MG217298

Cdk10 (GFP-tagged) - Mouse cyclin-dependent kinase 10 (Cdk10) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdk10
• Synonyms: BC017131
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217298 representing NM_194446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGTGGACCTGGAGTCGGATCAGATCCGATTGAAGTGTATCCGTAAGGAAGGTTTCTTCACCG
  TGCCTCCAGAACACAGGCTGGGAAGATGCCGAAGCGTTAAGGAGTTTGAGAAGCTGAACCGGATTGGCGA
  GGGCACCTATGGCATCGTGTATCGGGCCAGGGATACCCAGACAGATGAAATTGTCGCCCTGAAGAAGGTG
  CGGATGGACAAAGAGAAGGATGGCATCCCCATCAGCAGCCTGCGTGAGATCACACTGCTCTTGCGTCTCC
  GCCATCCAAACATTGTGGAGCTGAAGGAGGTGGTTGTGGGCAACCACCTGGAGAGCATCTTCCTGGTCAT
  GGGTTACTGCGAACAAGATCTGGCCAGCCTATTGGAAAATATGCCAACACCCTTCTCGGAGGCCCAGGTT
  AAATGCATCATGCTACAGGTGCTTCGTGGCCTTCAGTACCTGCACAGGAACTTCATCATCCACAGGGACC
  TGAAGGTGTCCAACTTGCTCATGACAGACAAGGGCTGTGTAAAGACAGCTGATTTTGGCCTGGCTCGGGC
  CTATGGTGTTCCAGTAAAGCCAATGACTCCCAAGGTTGTTACCCTCTGGTACCGAGCCCCAGAGCTGCTG
  CTTGGAACTACCACCCAGACTACCAGCATTGACATGTGGGCTGTCGGCTGCATCCTGGCAGAGCTGCTGG
  CCCATAAGCCCCTCCTCCCTGGCACTTCCGAGATCCACCAGATCGACTTGATTGTACAGCTGTTGGGGAC
  ACCGAGTGAGAATATCTGGCCGGGTTTCTCCAAGCTGCCGCTGGCCGGCCAGTACAGCTTGAGGAAACAG
  CCCTATAACAACCTCAAGCACAAGTTCCCGTGGCTCTCAGAGGCCGGACTCCGTCTGCTCAACTTCCTCT
  TCATGTATGACCCTAAGAAAAGGGCAACCTCGGGAGACTGCCTGGAGAGCTCCTACTTCAAGGAGAAGCC
  CCTGCCCTGTGAACCGGAGCTCATGCCTACCTTCCCCCACCACCGCAATAAGCGTGCTGCCCCAGCTGCC
  GCTGAAGGGCAGAGCAAACGATGCCGGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217298 representing NM_194446
  Red=Cloning site Green=Tags(s)
  
  MAEVDLESDQIRLKCIRKEGFFTVPPEHRLGRCRSVKEFEKLNRIGEGTYGIVYRARDTQTDEIVALKKV
  RMDKEKDGIPISSLREITLLLRLRHPNIVELKEVVVGNHLESIFLVMGYCEQDLASLLENMPTPFSEAQV
  KCIMLQVLRGLQYLHRNFIIHRDLKVSNLLMTDKGCVKTADFGLARAYGVPVKPMTPKVVTLWYRAPELL
  LGTTTQTTSIDMWAVGCILAELLAHKPLLPGTSEIHQIDLIVQLLGTPSENIWPGFSKLPLAGQYSLRKQ
  PYNNLKHKFPWLSEAGLRLLNFLFMYDPKKRATSGDCLESSYFKEKPLPCEPELMPTFPHHRNKRAAPAA
  AEGQSKRCRP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194446
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_194446.2, NP_919428.1
• RefSeq Size: 1661
• RefSeq ORF: 1083
• Locus ID: 234854
• Gene Summary: The protein encoded by this gene belongs to the CDK (cyclin-dependent kinase) subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. The human ortholog has been shown to play a role in cellular proliferation. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 1. [provided by RefSeq, Jan 2010]