Ccdc50 (NM_001025615) Mouse Tagged ORF Clone

CAT#: MG217752

Ccdc50 (GFP-tagged) - Mouse coiled-coil domain containing 50 (Ccdc50) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccdc50
• Synonyms: C3orf6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217752 representing NM_001025615
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGTGAGTGTAGATCAGTCCAAGTTGCCGGGCGTGAAGGAAGTGTGTAGAGATTTTGCCGTCC
  TGGAGGACCACACCCTGGCCCATAGCCTGCAGGAACAAGAGATTGAGCATCATTTGGCATCCAACATTCA
  GCGGAACCGTCTGGTACAACATGATCTGCAGGTTGCTAAGCAGCTCCAAGAGGAAGACCTCAAAGCCCAA
  GCTCAGCTCCAGAAGCGCTACAAAGCCCTTGAACAACATGATTGTGAAATTGCTCAGGAAATCCAGGAGA
  AGCTAACCATTGAGGCTGAGAGACGACGCATTCAGGAAAAGAAGGATGAGGACATAGCACGTCTTTTGCA
  AGAGAAGGAGCTACAGGAGGAGAAAAGGAGGAAGAAACACACTCCAGAGTTTTCTGGGGGCAGTGTTTTT
  GGAGATAACTACTATCATGAGGATGGAGGAATGAAACCAAGAGGAATAAAAGAAGCTGTCTCTACTCCAG
  CACGAGCAAGCCACAGGGACCAGGAGTGGTATGATGCTGAAATTGCCAGGAAATTGCAAGAAGAAGAACT
  TTTGGAAATTGCTCGACTTCTAATGGCTGAAGAAAAAAAAGCTTACAAGAAAGCCAAAGAGCGAGAAAAG
  TCATCTTTGGACAAAAGGAAACATGACCCTGAATGCAAGTTAAAAGCAAAGTCAGCCCACTCAAAGTCAA
  AAGAGGGTGATGAAGCACACCGCTCCAAGATTGACAGGCCATCAAGACCACCACCACCTACCATGATGGG
  CCTTGAGGACACAGATCCCACCCATTTTACAAACCAGCACAGTACAACATGGCATCTTCCAAAGTCAGAG
  TCCTCACAGAAAGGCTTCCATAACAAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217752 representing NM_001025615
  Red=Cloning site Green=Tags(s)
  
  MADVSVDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNIQRNRLVQHDLQVAKQLQEEDLKAQ
  AQLQKRYKALEQHDCEIAQEIQEKLTIEAERRRIQEKKDEDIARLLQEKELQEEKRRKKHTPEFSGGSVF
  GDNYYHEDGGMKPRGIKEAVSTPARASHRDQEWYDAEIARKLQEEELLEIARLLMAEEKKAYKKAKEREK
  SSLDKRKHDPECKLKAKSAHSKSKEGDEAHRSKIDRPSRPPPPTMMGLEDTDPTHFTNQHSTTWHLPKSE
  SSQKGFHNKQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025615
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025615.2, NP_001020786.1
• RefSeq Size: 6936 bp
• RefSeq ORF: 873 bp
• Locus ID: 67501
• Cytogenetics: 16 18.98 cM