Aptx (NM_025545) Mouse Tagged ORF Clone

CAT#: MG218020

Aptx (GFP-tagged) - Mouse aprataxin (Aptx) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aptx
• Synonyms: 2410016G21Rik; AA388047; FHA-HIT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218020 representing NM_025545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAAGCAGTAGCTAAGATGCGAGTGTGCTGGTTGGTGAGACAGGACAGCAGGCACCAGCGAATCA
  AACTTCCCCATTTGGAAGCTGTTGTGATTGGTCGAAGCCCAGAGACCAAGATCACAGATAAGAAATGTTC
  CCGACAGCAAGTACAGTTGAAAGCAGAGTGTAACAAGGGATATGTCAAAGTACAGCAGATGGGGGTCAAC
  CCCACCAGCATTGACTCGGGCGTCATCGGGAAGGACCAAGAGAAGAAGCTGCTGCCTGGTCAGGTTCTCC
  ACATGGTGAATGGACTTTATCCATACATCGTAGAGTTTGAGGAAGTGGCAGAGAGCCCTAACCTAACACA
  GAGGAAGAGAAAGAGGTCAGACTGTGATAGTGAGGAGATGGAAGCTGAGTCTGGGACAGGGCTGGCACCT
  GGGAGCAGCCCCAGCCAGTGCTCTGTGTCCCCTAAGAAGGACAAGAATGGAGCCACCAAAAAGGAATCAC
  TGGGCCACTGGAGTCAAGGCTTGAAGATGTCTATGAAAGACCCCAAAATGCAGGTTTACAAAGACGACCA
  GGTGGTGGTGATTAAGGATAAATACCCCAAGGCCCGTCACCACTGGCTGGTCTTACCGTGGGCCTCCATT
  TCCAGTCTGAAGGTTGTGACCAGTGAACACCTTGAACTTCTCAAACATATGCACGCTGTGGGGGAGAAGG
  TGATAGCAGATTTTGCTGGATCCAGCAAACTGCGCTTCCGATTGGGCTACCATGCCATTCCCAGCATGAG
  CCACGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAAAAGCATTGGAAT
  TCTTTTAATACAGAATACTTTCTGGAATCACAAGCTGTGATCAAGATGGTTCAGGAAGCCGGCAGAGTGA
  CTGTTAAAGATGGCACTTGTGAGCTCTTGAAGCTGCCTCTCCGTTGCCATGAGTGTCAGCAGCTGCTGCC
  TTCCATCCCGCAGCTGAAAGAGCACCTCAGGAAGCACTGGGGCGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218020 representing NM_025545
  Red=Cloning site Green=Tags(s)
  
  MPEAVAKMRVCWLVRQDSRHQRIKLPHLEAVVIGRSPETKITDKKCSRQQVQLKAECNKGYVKVQQMGVN
  PTSIDSGVIGKDQEKKLLPGQVLHMVNGLYPYIVEFEEVAESPNLTQRKRKRSDCDSEEMEAESGTGLAP
  GSSPSQCSVSPKKDKNGATKKESLGHWSQGLKMSMKDPKMQVYKDDQVVVIKDKYPKARHHWLVLPWASI
  SSLKVVTSEHLELLKHMHAVGEKVIADFAGSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWN
  SFNTEYFLESQAVIKMVQEAGRVTVKDGTCELLKLPLRCHECQQLLPSIPQLKEHLRKHWGG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025545
• ORF Size: 1026 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025545.4, NP_079821.3
• RefSeq Size: 5689
• RefSeq ORF: 1029
• Locus ID: 66408