Gcat (NM_001161712) Mouse Tagged ORF Clone

CAT#: MG218051

Gcat (GFP-tagged) - Mouse glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) (Gcat) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gcat
• Synonyms: AI526977; Kbl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218051 representing NM_001161712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGCCAGCTTCATGTGGCACGGGGCGCTGTCCCCTGGCCGCCGCGCACATTCAGCGCTGGCTCAGT
  TGCGCTGCATCCTGGACAGCGAACTGGAAGGGATCCGCGGAGCCGGCACCTGGAAGAGTGAGCGTGTGAT
  CACGTCCCGCCAGGGACCGAGCATCCGCGTGGACGGCATCTCGGGAGGAATCCTCAACTTCTGTGCCAAT
  AACTACCTGGGCCTGAGCAGCCACCCTGCAGTGATCCAGGCAGGTCTGCAGACTCTGGAGGAGTTTGGAG
  CTGGACTCAGTTCTACTCGATTTATCTGTGGGACCCAGAGCATCCATAAGAATCTAGAAGCCAAGATAGC
  CCACTTCCACCAGCGTGAGGACGCCATCCTCTATCCCAGCTGTTTCGATGCCAACGCTGGCCTATTTGAG
  GCTTTGCTGACCCCAGAGGATGCGGTTCTGTCAGATGAGCTGAACCATGCTTCCATCATTGACGGCATCC
  GACTGTGCAAGGCCCACAAGTACCGTTACCGCCACCTGGACATGGCTGACCTGGAAGCCAAGCTGAAGGA
  GGCTCAGAAGCACAGGCTGCGCCTGGTGGCCACAGATGGAGCTTTCTCCATGGATGGTGACATCGCTCCC
  CTACAGGACATCTGCCGCCTTGCCGCCCAATATGGTGCCCTGGTCTTTGTGGATGAATGCCATGCCACTG
  GCTTTCTTGGGCCCACAGGACGGGGCACAGATGAGCTGCTAGGTGTCATGGACCAGGTCACCATCATCAA
  CTCCACCCTGGGGAAGGCGCTGGGTGGAGCATCAGGGGGATATACCACCGGGCCTGAGCCACTGGTGTCC
  CTGCTACGGCAGCGTTCTCGGCCCTACCTCTTCTCCAACAGCCTGCCCCCTGCTGTAGTCGGCTGTGCTT
  CTAAGGCCCTGGACCTGCTCATGGAGAGCAATGCCATCATCCAGTCTATGGCTGCCAAGACCCGGCGGTT
  CCGCAGTAAGATGGAAGCTGCTGGTTTTACTGTCTCAGGAGCCGATCACCCCATCTGCCCTGTGATGCTG
  GGAGATGCACGGTTGTCTTCTCAAATGGCAGATGACATGTTAAAGAAAGGAAAATGGCTGACATCCAGAA
  CATTTCGCTGGACAGCCCAGGGAGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218051 representing NM_001161712
  Red=Cloning site Green=Tags(s)
  
  MWASFMWHGALSPGRRAHSALAQLRCILDSELEGIRGAGTWKSERVITSRQGPSIRVDGISGGILNFCAN
  NYLGLSSHPAVIQAGLQTLEEFGAGLSSTRFICGTQSIHKNLEAKIAHFHQREDAILYPSCFDANAGLFE
  ALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLKEAQKHRLRLVATDGAFSMDGDIAP
  LQDICRLAAQYGALVFVDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPEPLVS
  LLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQSMAAKTRRFRSKMEAAGFTVSGADHPICPVML
  GDARLSSQMADDMLKKGKWLTSRTFRWTAQGA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161712
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161712.1, NP_001155184.1
• RefSeq Size: 1763
• RefSeq ORF: 1149
• Locus ID: 26912