Nell1 (NM_001037906) Mouse Tagged ORF Clone

CAT#: MG218170

Nell1 (GFP-tagged) - Mouse NEL-like 1 (chicken) (Nell1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nell1
• Synonyms: B230343H07Rik; l7R6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218170 representing NM_001037906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGATGGATGTGATTTTAGTTTTGTGGTTCTGTGTGTGCACCGCCAGGACAGTGCTGGGCTTTGGGA
  TGGACCCTGACCTTCAGATGGACATCATCACTGAACTTGACCTTGTGAACACCACCCTGGGCGTCACTCA
  GGTGGCTGGACTACACAATGCCAGTAAGGCATTTCTGTTTCAAGATGTACAGAGAGAGATCCACTCAGCC
  CCTCATGTGAGTGAGAAGCTGATCCAGCTATTCCGGAATAAGAGTGAGTTTACCTTTTTGGCTACAGTGC
  AGCAGAAGCCGTCCACCTCAGGGGTGATACTGTCGATCCGGGAGCTGGAACACAGCTATTTTGAACTGGA
  GAGCAGTGGCCCAAGAGAAGAGATACGCTATCATTACATCCATGGCGGCAAGCCCAGGACTGAGGCCCTT
  CCCTACCGCATGGCCGATGGACAGTGGCACAAGGTCGCGCTGTCTGTGAGCGCCTCTCACCTCCTACTCC
  ATGTCGACTGCAATAGGATTTATGAGCGTGTGATAGATCCTCCGGAGACCAACCTTCCTCCAGGAAGCAA
  TCTATGGCTTGGGCAACGTAATCAAAAGCATGGCTTTTTCAAAGGAATCATCCAAGATGGCAAGATCATC
  TTCATGCCGAACGGCTTCATCACACAGTGCCCCAACCTAAATCGCACTTGCCCAACATGCAGTGATTTCC
  TGAGCCTGGTTCAAGGAATAATGGATTTGCAAGAGCTTTTGGCCAAGATGACTGCAAAACTGAATTATGC
  AGAGACGAGACTTGGTCAACTGGAAAATTGCCACTGTGAGAAGACCTGCCAAGTGAGTGGGCTGCTCTAC
  AGGGACCAAGACTCCTGGGTAGATGGTGACAACTGCAGGAACTGCACATGCAAAAGTGGTGCTGTGGAGT
  GCCGAAGGATGTCCTGTCCCCCACTCAACTGTTCCCCAGACTCACTTCCTGTGCATATTTCTGGCCAATG
  TTGTAAAGTTTGCAGACCAAAATGTATCTATGGAGGAAAAGTTCTTGCTGAGGGCCAGCGGATTTTAACC
  AAGACCTGCCGGGAATGTCGAGGTGGAGTCTTGGTAAAAATCACAGAAGCTTGCCCTCCTTTGAACTGCT
  CAGAGAAGGATCATATTCTTCCGGAGAACCAGTGCTGCAGGGTCTGCCGAGGTCATAACTTCTGTGCAGA
  AGCACCTAAGTGTGGAGAAAACTCGGAATGCAAAAATTGGAATACAAAAGCGACTTGTGAGTGCAAGAAT
  GGATACATCTCTGTCCAGGGCAACTCTGCATACTGTGAAGATATCGATGAGTGTGCAGCAAAGATGCACT
  ACTGTCATGCCAACACGGTGTGTGTCAACTTGCCGGGGTTATATCGCTGTGACTGCATCCCAGGATACAT
  CCGTGTGGATGACTTCTCTTGTACGGAGCATGATGATTGTGGCAGCGGACAACACAACTGTGACAAAAAT
  GCCATCTGTACCAACACAGTCCAGGGACACAGCTGTACCTGCCAGCCAGGCTACGTGGGAAATGGTACTG
  TCTGCAAAGCATTCTGTGAAGAGGGTTGCAGATACGGAGGTACCTGTGTGGCCCCTAACAAATGTGTCTG
  TCCTTCTGGATTCACAGGAAGCCACTGTGAGAAAGATATTGATGAATGTGCAGAGGGATTCGTTGAGTGC
  CACAACCACTCCCGCTGCGTTAACCTTCCAGGGTGGTACCACTGTGAGTGCAGAAGCGGTTTCCATGACG
  ATGGGACCTATTCACTGTCCGGGGAGTCCTGCATTGATATTGATGAATGTGCCTTAAGAACTCACACTTG
  TTGGAATGACTCTGCCTGCATCAACTTAGCAGGAGGATTTGACTGCCTGTGTCCCTCTGGGCCCTCCTGC
  TCTGGTGACTGTCCCCACGAAGGGGGGCTGAAGCATAATGGGCAGGTGTGGATTCTGAGAGAAGACAGGT
  GTTCAGTCTGTTCCTGTAAGGATGGGAAGATATTCTGCCGGCGGACAGCTTGTGATTGCCAGAATCCAAA
  TGTTGACCTTTTCTGCTGCCCAGAGTGTGACACCAGGGTCACTAGCCAATGTTTAGATCAAAGCGGACAG
  AAGCTCTATCGAAGTGGAGACAACTGGACCCACAGCTGCCAGCAGTGCCGATGTCTGGAAGGAGAGGCAG
  ACTGCTGGCCTCTAGCTTGCCCTAGTTTGAGCTGTGAATACACAGCCATCTTTGAAGGAGAGTGTTGTCC
  CCGCTGTGTCAGTGACCCCTGCCTGGCTGATAATATTGCCTATGACATCAGAAAAACTTGCCTGGACAGC
  TCTGGTATTTCGAGGCTGAGCGGCGCAGTGTGGACAATGGCTGGATCTCCCTGTACAACCTGTCAATGCA
  AGAATGGGAGAGTCTGCTGCTCTGTGGATCTGGTGTGTCTTGAGAATAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218170 representing NM_001037906
  Red=Cloning site Green=Tags(s)
  
  MPMDVILVLWFCVCTARTVLGFGMDPDLQMDIITELDLVNTTLGVTQVAGLHNASKAFLFQDVQREIHSA
  PHVSEKLIQLFRNKSEFTFLATVQQKPSTSGVILSIRELEHSYFELESSGPREEIRYHYIHGGKPRTEAL
  PYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPETNLPPGSNLWLGQRNQKHGFFKGIIQDGKII
  FMPNGFITQCPNLNRTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLGQLENCHCEKTCQVSGLLY
  RDQDSWVDGDNCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHISGQCCKVCRPKCIYGGKVLAEGQRILT
  KTCRECRGGVLVKITEACPPLNCSEKDHILPENQCCRVCRGHNFCAEAPKCGENSECKNWNTKATCECKN
  GYISVQGNSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCIPGYIRVDDFSCTEHDDCGSGQHNCDKN
  AICTNTVQGHSCTCQPGYVGNGTVCKAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECAEGFVEC
  HNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSC
  SGDCPHEGGLKHNGQVWILREDRCSVCSCKDGKIFCRRTACDCQNPNVDLFCCPECDTRVTSQCLDQSGQ
  KLYRSGDNWTHSCQQCRCLEGEADCWPLACPSLSCEYTAIFEGECCPRCVSDPCLADNIAYDIRKTCLDS
  SGISRLSGAVWTMAGSPCTTCQCKNGRVCCSVDLVCLENN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037906
• ORF Size: 2430 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037906.2, NP_001032995.1
• RefSeq Size: 2812 bp
• RefSeq ORF: 2433 bp
• Locus ID: 338352
• Cytogenetics: 7 B4