Prodh (NM_011172) Mouse Tagged ORF Clone

CAT#: MG218491

Prodh (GFP-tagged) - Mouse proline dehydrogenase (Prodh) nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prodh
• Synonyms: Pro-1; Pro1; Ym24d07
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218491 representing NM_011172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCAAGCGCGTCTTCTTGCTGCGGTCGGTGGCACCACGCGTCGCTGCCCTCTCAACCAAACCGC
  AAGCCCAGGAACAGCCTCCCGCGAGCCCTGAGGCTCTTCGGGGATGTGGGGCGGCCAAGGCTGTGCGGCC
  GCCTGTGCCAGCCGTGGACTTCACCAACACGCAGGAGGCGTATCGCAGCCGGCGGAGTTGGGAGTTGGTG
  CGCAACCTGCTAGTGCTGCGGCTGTGTGCGTCGCCGGTGCTGCTAGCGCACCACGAGCAGTTGTTCCAAG
  TTGCCAGGAAGCTTCTGGGGCAAAGGATGTTCGAGAGATTGATGAAGATGACCTTCTATGGCCATTTTGT
  GGCTGGCGAGGACCAGGAGTCTATCAGGCCTCTGATCCGGCACAACAAAGCCTTTGGTGTTGGCTTTATC
  CTGGACTATGGAGTGGAGGAAGATCTGAGCCCTGAGGAGGCGGAGCGCAAAGAGATGGAGTCATGCACTT
  CTGAAGCAGAGAGAGATGGCAGTGGAGCAAATAAGAGGGAGAAGCAGTATCAGGTGCACCCCGCCTTTGG
  AGACCGCAGAGATGGTGTCATCAGTGCCCGCACCTACTTCTATGCCAATGAAGCCAAGTGTGACAACTAC
  ATGGAGAACTTACTGCAGTGCATCAAGGCCTCAGGTGGAGCCAGTGATGGTGGTTTCTCAGCCATTAAGC
  TCACTGCACTGGGGAGACCACAGTTTCTGCTGCAGTTCTCAGACGTGCTGACCAGGTGGAGACGGTTCTT
  CCATCAAATGGCTGCAGAGCAGGGACAGGCTGGGCGTGCTGCTGTAGACACAAAGCTGGAGGTGGCGGTG
  CTCCAGGACAGCATCGCAAAGATGGGCATCGCATCCAGGGCTGAGATTGAAGGGTGGTTCACGCCAGAGA
  CGCTGGGAGTGTCTGGCACCGTGGACTTGCTGGACTGGAACAGCCTCATTGACAGCAGGACCCGGCTCTC
  CAGGCACTTGGTGGTCCCCAATGTGCAGACTGGCCAGCTGGAGCCCCTGCTGTCACGGTTCACTGAGGAG
  GAAGAGCAGCAGATGAAAAGGATGCTGCAGAGGATGGATGTACTGGCCAAGAAAGCAAAAGAGGCAGGTG
  TGCGCCTGATGATTGATGCTGAGCAGAGCTACTTCCAACCAGCCATCAGCCGCCTGACCCTGGAGATGCA
  GCGCAGGTTCAATGTGGATAAGCCGTTCATCTTCAACACATTCCAGTGCTACCTCAAGGATGCCTATGAC
  AATGTGACCTTGGATATGGAACTGGCTCGCCGTGAGGGCTGGTGTTTTGGGGCCAAGCTGGTACGTGGTG
  CATACATGGCCCAAGAGCGTGTCAGGGCAGCAGAGATCGGTTATGAGGACCCCATCAACCCTACATATGA
  AGCCACCAATGCTATGTACCACAGGTGCCTTAACTATGTTCTGGAGGAGCTGAAGCACAGCACCAAGGCA
  GAGGTGATGGTGGCTTCCCACAACGAGGACACCGTGCACTTCACGTTGTGCAGGATGAAGGAGATAGGCC
  TGCATCCTGCTGATGGTCAGGTGTGCTTCGGACAGCTGCTGGGGATGTGTGACCAAATCAGCTTCCCACT
  AGGCCAGGCAGGCTTTCCTGTGTACAAGTATGTGCCCTATGGCCCTGTGATGGAGGTACTCCCTTACCTG
  TCCCGCCGTGCCCTGGAGAACAGCAGCATCATGAAGGGTGCTCAGCGAGAGAGGCAGCTGCTATGGCAGG
  AGCTCCGCAGGCGGCTGCGCACTGGCAGCCTCTTCCACCATCCGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218491 representing NM_011172
  Red=Cloning site Green=Tags(s)
  
  MALKRVFLLRSVAPRVAALSTKPQAQEQPPASPEALRGCGAAKAVRPPVPAVDFTNTQEAYRSRRSWELV
  RNLLVLRLCASPVLLAHHEQLFQVARKLLGQRMFERLMKMTFYGHFVAGEDQESIRPLIRHNKAFGVGFI
  LDYGVEEDLSPEEAERKEMESCTSEAERDGSGANKREKQYQVHPAFGDRRDGVISARTYFYANEAKCDNY
  MENLLQCIKASGGASDGGFSAIKLTALGRPQFLLQFSDVLTRWRRFFHQMAAEQGQAGRAAVDTKLEVAV
  LQDSIAKMGIASRAEIEGWFTPETLGVSGTVDLLDWNSLIDSRTRLSRHLVVPNVQTGQLEPLLSRFTEE
  EEQQMKRMLQRMDVLAKKAKEAGVRLMIDAEQSYFQPAISRLTLEMQRRFNVDKPFIFNTFQCYLKDAYD
  NVTLDMELARREGWCFGAKLVRGAYMAQERVRAAEIGYEDPINPTYEATNAMYHRCLNYVLEELKHSTKA
  EVMVASHNEDTVHFTLCRMKEIGLHPADGQVCFGQLLGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYL
  SRRALENSSIMKGAQRERQLLWQELRRRLRTGSLFHHPA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011172
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011172.2, NP_035302.2
• RefSeq Size: 2283
• RefSeq ORF: 1800
• Locus ID: 19125
• Gene Summary: Converts proline to delta-1-pyrroline-5-carboxylate. [UniProtKB/Swiss-Prot Function]