Nek3 (NM_001162947) Mouse Tagged ORF Clone

CAT#: MG218615

Nek3 (GFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 3 (Nek3) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nek3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218615 representing NM_001162947
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAACTACACAGTCCTGAGAGTGATTGGCCAGGGCTCCTTCGGCAGGGCTCTTTTGGTTCTGCAGG
  AAAGCAGCAATCAGACATTTGCCATGAAGGAAATCAGACTGCTCAAGTCTGATACACAGACTTCTAGGAA
  AGAGGCTGTTCTGCTCGCCAAAATGAAGCACCCCAATATTGTTGCCTTTAAGGAGTCATTTGAAGCTGAA
  GGGTACCTGTACATTGTGATGGAGTATTGTGACGGAGGGGATCTGATGCAGAGGATTAAACAGCAAAAAG
  GGAAGTTATTCCCTGAAGACACGATACTTAATTGGTTTATACAAATTTGTCTTGGAGTAAATCACATTCA
  TAAGAGACGTGTTTTACACAGAGATATCAAGTCGAAAAATGTCTTCCTCACCCACAATGGAAAAGTGAAA
  TTGGGAGATTTTGGCTCTGCCCGTCTTCTCTCCAGTCCCATGGCGTTTGCATGCACGTACGTGGGAACAC
  CTTATTATGTGCCTCCAGAAATCTGGGAGAACCTGCCTTACAACAATAAAAGTGACATCTGGTCCTTGGG
  ATGCATTCTGTATGAACTCTGCGCTCTGAAGCATCCATTCCAGGCAAATAGTTGGAAAAACCTGATCCTG
  AAAATATGCCAGGGGCCCATCCACCCGCTGCCGGCTCTGTACTCCTGCAAGCTTCAGGGCCTGGTCAAGC
  AGATGCTGAAAAGGAACCCTTCCCACCGCCCCTCCGCCACAACCCTGCTCTGCAGGGGTAGCTTGGCTCC
  ACTGGTCCCGAAGTGCTTGCCCCCTCAGATCATCAGAGAATATGGAGAACAGATATTAGATGAAATCAAA
  ATATCCACGCCTAAGAATATGAAAAAACAGGACTCCAACAGAGTCAGGAGAGCTCTGGGAGAAGCAAACT
  CTGCCTCTATGCAAGAGGAAGAACGTGGTAGAAAGTGTAGCCATACTGAATTGGAAAGCACTGGTACAAC
  CCCAGCTGGGAATGCATTGGAAAGAGCAGCCAGAGGAAATCCAGAATCAGGTAACCCACAGGAGCACGGG
  AGGCATACCAGTCCGGCAAGTCCTCACAGGCCGTGGTGGGAGAGACACGGTCCCAGTTCCAATGTCGAAG
  CTTTGGAGAAAGCATCTATCCTTACCTCCAGTTTCACGGCAGAGGATGACAGAGGTGGGTCTGTGATAAA
  GTATGAAGAAAATGCCCGAAGGCAGTGGGTCAGAGAGCCCCCTGAGGCCTTGCTGTCAATGCTGAAGGAC
  GCTGATCTCAGTCAGGCATTTCAGACATACACAATATATAGACCAGGTGCAGAAGGATTCCTGAAAGGTC
  CCCTTTCTGAAGACACGGCATCGGACAGCGTCGACGGTGATCTCGATTCTGTCATGTTAGACCCAGAGAG
  ATTTGAACCTCGGCTGGATGAAGAAGACACGGACTTTGAGGAAGATAACGAAAACCCTGATTGGGTGTCA
  GAACTAAAGAAGCACGTAGGATACGGGGATGGACCTGGTGGACAACTCCTCGGAGAACGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218615 representing NM_001162947
  Red=Cloning site Green=Tags(s)
  
  MDNYTVLRVIGQGSFGRALLVLQESSNQTFAMKEIRLLKSDTQTSRKEAVLLAKMKHPNIVAFKESFEAE
  GYLYIVMEYCDGGDLMQRIKQQKGKLFPEDTILNWFIQICLGVNHIHKRRVLHRDIKSKNVFLTHNGKVK
  LGDFGSARLLSSPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCALKHPFQANSWKNLIL
  KICQGPIHPLPALYSCKLQGLVKQMLKRNPSHRPSATTLLCRGSLAPLVPKCLPPQIIREYGEQILDEIK
  ISTPKNMKKQDSNRVRRALGEANSASMQEEERGRKCSHTELESTGTTPAGNALERAARGNPESGNPQEHG
  RHTSPASPHRPWWERHGPSSNVEALEKASILTSSFTAEDDRGGSVIKYEENARRQWVREPPEALLSMLKD
  ADLSQAFQTYTIYRPGAEGFLKGPLSEDTASDSVDGDLDSVMLDPERFEPRLDEEDTDFEEDNENPDWVS
  ELKKHVGYGDGPGGQLLGERA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162947
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001162947.1, NP_001156419.1
• RefSeq Size: 1989
• RefSeq ORF: 1536
• Locus ID: 23954