St8sia5 (NM_013666) Mouse Tagged ORF Clone

CAT#: MG218712

St8sia5 (GFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-28-sialyltransferase 5 (St8sia5) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: St8sia5
• Synonyms: Siat8e; ST8SiaV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218712 representing NM_013666
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTACGCAGACCCCTCCGCCAACAGAGATTTGTTGGGGAATCGAACTTTGCTCTTTATCTTCATCT
  GCGCCTTTGCCTTGGTGACCTTGCTTCAGCAGATCCTGTACAGCAAGAGCTACATCAAAAGAGGCTTCCA
  GTTTGGTTGGCAGAGGGGCGACCAACAGGCCAATTGGACTGGGCTCTTTAATGACAGCGACAGCCCAACA
  GAGCAGAACATCACTGGCTCCAGCTCCAGGTACTTCGAGTTTTACAAGGAGCCTTTAGAGTTTAACTCCA
  CGAGATGCCTGGAGCTGAGGCAGGAGATCCTGGAGGTGAAGGTGCTGTCCATGGTGAAGCAGTCTGAACT
  CTTCGAAAGGTGGAAGAGTCTCCAGATATGCAAATGGGCAATGGACACCGCGGAAGCCAGCCTCTTCAAG
  TCCACCTTGTCCAGGTGCTGCAATGCCCCCAGCTTTCTCTTCACCACCCAGAAGAACACCCCGGTGGAGA
  CGAATCTCAGGTATGAGGTGGAATCCAGCGGCTTATATCACATCGACCAGGAGATCTTCAAAATGTTTCC
  CAAGGAAATGCCCTACTACCGCTCTCAGTTTAAGAAGTGTGCTGTGGTGGGCAATGGCGGCATCTTGAAG
  AACAGCGGCTGCGGGAAGGAGATCAACAGTGCTGACTTTGTCTTCCGGTGCAACCTGCCCCCCATCTCAG
  GGATATATACCACAGATGTGGGAGAGAAGACAGATGTAGTCACCGTGAATCCCAGCATCATAATTGACAG
  GTTCCACAAGTTGGAGAAGTGGCGGCGGCCTTTCTTCAGCGTGCTGCAGAGGTACGAGAACGCCTCAGTG
  CTGCTGCCAGCGTTCTACAACGTCCGCAACACCCTGGTATCCTTCCGAGTCAAATACATGCTGGACGACT
  TCCAGTCGCGGCAGCCGGTCTACTTCTTCCACCCGCAGTACCTGAGCAGTGTGTCGCGCTACTGGCTCAG
  CCTGGGTGTGCGCGCGCGGCGTATCAGCACCGGCCTCATCCTGGTCACCGCGGCCCTGGAGCTCTGCGAG
  GAGGTGCACCTGTTCGGTTTCTGGGCCTTCCCCATGAACCCCTCCGGCTTCTTCATCACGCACCATTACT
  ATGACAACGTCAAGCCCAAGCCGGGTTTCCATGCTATGCCCTCGGAGATCTTCACCTTCCTGCGCATGCA
  CAGCCGCGGCATCCTTCGCGTGCACACAGGCACCTGCAATTGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218712 representing NM_013666
  Red=Cloning site Green=Tags(s)
  
  MRYADPSANRDLLGNRTLLFIFICAFALVTLLQQILYSKSYIKRGFQFGWQRGDQQANWTGLFNDSDSPT
  EQNITGSSSRYFEFYKEPLEFNSTRCLELRQEILEVKVLSMVKQSELFERWKSLQICKWAMDTAEASLFK
  STLSRCCNAPSFLFTTQKNTPVETNLRYEVESSGLYHIDQEIFKMFPKEMPYYRSQFKKCAVVGNGGILK
  NSGCGKEINSADFVFRCNLPPISGIYTTDVGEKTDVVTVNPSIIIDRFHKLEKWRRPFFSVLQRYENASV
  LLPAFYNVRNTLVSFRVKYMLDDFQSRQPVYFFHPQYLSSVSRYWLSLGVRARRISTGLILVTAALELCE
  EVHLFGFWAFPMNPSGFFITHHYYDNVKPKPGFHAMPSEIFTFLRMHSRGILRVHTGTCNCC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013666
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013666.2, NP_038694.2
• RefSeq Size: 2114
• RefSeq ORF: 1239
• Locus ID: 225742
• Gene Summary: May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively. [UniProtKB/Swiss-Prot Function]