Adar (NM_001038587) Mouse Tagged ORF Clone

CAT#: MG218722

Adar (GFP-tagged) - Mouse adenosine deaminase RNA-specific (Adar) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adar
• Synonyms: Adar1; Adar1p110; Adar1p150; AV242451; mZaADAR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218722 representing NM_001038587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAATCAAGGAGAAGATCTGTGACTATCTGTTCAATGTGTCAAACTCCTCTGCCCTGAACCTGG
  CTAAGAACATTGGCCTCACCAAGGCCCGAGATGTGACCTCAGTGCTGATTGACTTGGAAAGGCAAGGCGA
  TGTCTACAGGCAAGGGGCAACTCCTCCCATCTGGTACTTGACGGACAAGAAGCGTGAGAGGCTGCAGATG
  AAGAGAAGTACACACAGTGCTCCTGCCCCTACCCCGACAGCTGTCCCAGAGGCCACTAGAAGCCCCTCAT
  TCCCTGCCTGCCACCCGCCCCCAGCAGGTGCCTCAAGCAGTGTGGCAGCCTCCAAGAGAGTGGAGAATGG
  GCAGGAGCCTGCGATAAAGCATGAAAGTAGGCATGAGGCCAGACCAGGACCAATGAGACTGCGGCCTCAC
  GCTTATCACAATGGCCCCTCTAGAGCAGGGTATGTGGCCTCTGAAAATGGCCAGTGGGCCACAGATGACA
  TCCCAGATAACTTGAATAGTATCCACACAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCCTCCTT
  CTACAGCCCTACCTTGCCACGGTGTTCACCCTACAAGAAGCTAACTGAGTGCCAGCTGAAGAACCCTGTC
  AGCGGGTTGTTAGAGTATGCTCAGTTCACTAGTCAGACCTGTGATTTCAACCTGATAGAGCAGAGTGGAC
  CGTCCCATGAACCTCGATTTAAATTCCAGGTTGTCATCAATGGGCGGGAATTTCCCCCAGCTGAGGCTGG
  CAGCAAGAAAGTAGCCAAGCAGGACGCAGCAGTGAAAGCCATGGCGATTCTGCTTCGGGAAGCCAAAGCC
  AAAGACAGTGGTCAACCAGAAGACTTGTCCCACTGTCCCATGGAAGAAGACTCGGAGAAACCAGCAGAGG
  CTCAGGCCCCCAGCTCCTCAGCAACATCCTTGTTCTCTGGGAAGAGCCCAGTTACTACACTGCTTGAGTG
  CATGCACAAACTAGGGAACTCCTGTGAATTCCGTCTCCTGTCCAAAGAAGGCCCTGCTCATGACCCCAAG
  TTCCAGTACTGTGTAGCAGTAGGAGCCCAGACCTTCCCCCCTGTGAGCGCCCCCAGCAAGAAGGTAGCAA
  AGCAGATGGCCGCAGAGGAAGCCATGAAGGCGCTGCAGGAGGAGGCAGCCAGTTCAGCGGATGACCAGTC
  TGGAGGTGCGAACACAGACTCACTTGATGAATCTATGGCTCCCAACAAGATCAGGAGGATTGGTGAGCTC
  GTCAGGTACCTGAACACCAACCCCGTAGGCGGCTTGTTGGAGTACGCCCGATCTCATGGCTTTGCTGCTG
  AGTTCAAGCTCATTGACCAGTCTGGACCTCCTCACGAACCCAAGTTTGTTTACCAAGCAAAAGTTGGGGG
  CCGCTGGTTTCCAGCCGTGTGTGCACACAGCAAGAAACAGGGCAAGCAGGATGCAGCGGATGCAGCCCTC
  CGGGTCTTGATCGGGGAGAGCGAGAAGGCAGAGCAGTTGGGTTTCGCAGAGGTAACCCCAGTAACAGGGG
  CCAGTCTCAGAAGAACTATGCTCCTCCTTTCCAGGTCCCCAGATGCACATCCAAAGACACTTCCTCTCTC
  TGGCAGCACCTTCCACGACCAGATAGCTATGCTGAGCCACAGGTGCTTCAATGCTCTGACCAACAGTTTC
  CAGCCCTCCCTGCTCGGCCGCAAGATCCTGGCTGCCATTATTATGAAAAGAGATCCAGAGGACATGGGTG
  TTGTCGTGAGTTTGGGGACAGGCAATCGCTGTGTGAAAGGGGACTCTCTCAGCCTGAAGGGAGAGACGGT
  CAATGACTGCCATGCCGAAATCATCTCCCGGAGGGGCTTCATCAGGTTTCTCTACAGTGAACTGATGAAG
  TACAACCACCACACTGCCAAGAACAGCATATTTGAGCTTGCCAGGGGAGGAGAGAAGCTGCAGATAAAGA
  AGACGGTTTCTTTTCATCTCTACATCAGCACGGCGCCATGTGGAGATGGGGCCCTCTTTGACAAATCCTG
  CAGTGACCGTGCCGTGGAAAGCACAGAGTCCCGCCATTACCCTGTCTTTGAAAATCCCAAGCAAGGCAAG
  CTTCGCACCAAGGTGGAGAATGGGGAAGGCACAATTCCTGTGGAGTCCAGTGATATTGTACCCACGTGGG
  ATGGCATCCGCCTTGGGGAAAGACTCCGTACCATGTCCTGTAGTGACAAAATCCTACGCTGGAATGTGCT
  GGGCCTGCAAGGGGCGTTGTTGACGCACTTCCTACAGCCTGTGTACCTGAAATCTGTAACCTTAGGTTAC
  CTTTTCAGCCAAGGGCATCTGACCCGTGCTATTTGCTGCCGCGTGACCAGAGATGGGAAAGCATTTGAGG
  ATGGACTACGCTATCCCTTTATTGTCAACCACCCCAAGGTCGGCCGAGTCAGTGTTTATGATTCCAAAAG
  ACAGTCCGGAAAGACCAAGGAAACAAGCGTCAACTGGTGCATGGCTGATGGCTATGACCTAGAGATCCTG
  GATGGCACCAGAGGCACTGTGGATGGACCAGGGAAAGAGTTGTCTCGGGTGTCCAAGAAGAATATTTTCC
  TTCAGTTTAAGAAGCTCTGCTCCTTCCGAGCCCGCAGAGATTTACTGCAGCTCTCTTATGGTGAAGCCAA
  GAAAGCTGCCCGTGACTATGACTTAGCCAAGAACTACTTCAAGAAAAGCCTGCGAGACATGGGCTATGGG
  AATTGGATCAGCAAACCCCAGGAGGAAAAGAACTTTTACCTCTGTCCAGTACCCAATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218722 representing NM_001038587
  Red=Cloning site Green=Tags(s)
  
  MAEIKEKICDYLFNVSNSSALNLAKNIGLTKARDVTSVLIDLERQGDVYRQGATPPIWYLTDKKRERLQM
  KRSTHSAPAPTPTAVPEATRSPSFPACHPPPAGASSSVAASKRVENGQEPAIKHESRHEARPGPMRLRPH
  AYHNGPSRAGYVASENGQWATDDIPDNLNSIHTAPGEFRAIMEMPSFYSPTLPRCSPYKKLTECQLKNPV
  SGLLEYAQFTSQTCDFNLIEQSGPSHEPRFKFQVVINGREFPPAEAGSKKVAKQDAAVKAMAILLREAKA
  KDSGQPEDLSHCPMEEDSEKPAEAQAPSSSATSLFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHDPK
  FQYCVAVGAQTFPPVSAPSKKVAKQMAAEEAMKALQEEAASSADDQSGGANTDSLDESMAPNKIRRIGEL
  VRYLNTNPVGGLLEYARSHGFAAEFKLIDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQDAADAAL
  RVLIGESEKAEQLGFAEVTPVTGASLRRTMLLLSRSPDAHPKTLPLSGSTFHDQIAMLSHRCFNALTNSF
  QPSLLGRKILAAIIMKRDPEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMK
  YNHHTAKNSIFELARGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAVESTESRHYPVFENPKQGK
  LRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPVYLKSVTLGY
  LFSQGHLTRAICCRVTRDGKAFEDGLRYPFIVNHPKVGRVSVYDSKRQSGKTKETSVNWCMADGYDLEIL
  DGTRGTVDGPGKELSRVSKKNIFLQFKKLCSFRARRDLLQLSYGEAKKAARDYDLAKNYFKKSLRDMGYG
  NWISKPQEEKNFYLCPVPND
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001038587
• ORF Size: 2790 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038587.4, NP_001033676.2
• RefSeq Size: 5936 bp
• RefSeq ORF: 2793 bp
• Locus ID: 56417
• Cytogenetics: 3 F1