Dcp1b (NM_001033379) Mouse Tagged ORF Clone

CAT#: MG218839

Dcp1b (GFP-tagged) - Mouse DCP1 decapping enzyme homolog b (S. cerevisiae) (Dcp1b), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dcp1b
• Synonyms: B930050E02Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218839 representing NM_001033379
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCGGCGGCAGGTGGCCTGCCTGGGAAGGGACACGATATCAGCCTGGCAGCCCTGCGGCGCC
  ACGACCCCTACATCAGTCGCATCGTGGACGTGGCCAGCCAGGTGGCTCTGTACACTTTCGGCCATCGGGC
  CAACGAGTGGGAGAAGACTGGAGTGGAGGGAACCTTGTTTGTTTATACGAGGTCTGCCTCCCCAAAGCAC
  GGATTCACCATTATGAATAGGCTCAGCATGGAAAACAGAACAGAACCCATTACTAAAGACCTGGACTTCC
  AGCTCCAGAACCCTTTCCTTCTCTACAGAAACGGCACACTGTCCATTTATGGAATTTGGTTTTATGATAA
  GGAAGAGTGCCAGAGAATTGCAAAACTAATGAAAAACCTCACTCAGAGCGAACAGTTGAAAGCCTGTCAT
  GGAGCCGGATCCTCCCCCGTGACCTTGAGCTCAGGAGAGGGCCAAGAAGTAGATATCCTGCAGATGCTCA
  CCAAGGCCAAGGATGAGTACACCAAGTGTAAGACCTGTTCCGAGCCAAAACAGATGACCAATTCCTCTGC
  CATCTGTGACAACCCTAAACTTATCAAACCTGTCCCCGTGAGACCCAGCAGCAGCCAGAGGCTGCAAGGA
  CCTGCGCCCAGCAAGACCTCGGACCCTGAGCCTCAGCACTTATCTTTAACAGCACTATTTGGGAAACAAG
  ACAAAGCTCCCTGTCAGGAAACTGTAAAGCCCTCCCGGACCTTTGCCCACCACCACCACCATCACCACCA
  GCAGCAGCAAGAGACGCGTCCAGTTCATCACGGGGTCGCATGCTCCCTGTCCTGTGAGGAGCCCAGGAAG
  CTCTCACTCCCGGTGGAGAAGCAGCTCTGCCCGGCCATTCAGAAACTCATGCTCGGGAGCCCGGGGCTGC
  ACCCCCTGCCTCAGCACCCTGAACAGTGGTCCTGCAAAAGTGGCAGCCCCAGTCCTGCCGGGGGAATTCT
  CCCTGGTCCTGTCCAGCTGGGGTCCCCCTGGAATGGCAGGGTTGCACACTGCACACAGAGCACTTGCAGA
  GGCCACAAGCTATTAGAGCAGCTTCAAGGTGCCCCTGGGGCAGTGCATAAGTATAACCCCTGTGCGCCTA
  CCGGTCCAGCTGTGGCCACTCAGGTGGCTCCGGGCCAGAGTGTGGCCCAATCACAGCTGGTGTATTTCAG
  TGGCCCCCTTCAACCTCCCGCCCCAGGACACCAAGCTCTCAGGAAGGAGCAGGGTGCACTGCCTGCACAA
  GCGGTGTCCCTCTCTGGCAGCCAGGAGAGCAGCCCCACCGTGCTCCCTACGCAGGAGCTGCTAAGGAAGC
  TCCAGGTAGTACACCAGGAGCAGCAGGCGGCCCCCCGGCCAGCCTTGGCAGCCAGGTTCCCTGTGTCAGC
  CCAGGGATCAGGGACAGAAAAGCCCTTGGAAGCCTGGGTCAGCAAGACAGCCAGCATGGAGAAGCAGGCT
  CCTCTTCTGCAGTCTACCTGTGCCCCACTGAGGGAGACTGATAATGGGCTCATGGCCCTAGGAGGCCAAG
  AACTTCCTGCTGCCTCCTCCAGCCTCCTCCTGCCCCTGCAGAACTGGGAGTCCTCCACCGTGGCCAGCAG
  GCCCCTCACCAGGCTGCAGCTCCAGGAGGCACTGCTGAACCTCATCCAGAATGATGACAACTTCTTAAGC
  ATAATCTATGAAGCCTATCTCTTCAGCGTGACACAAGCAGCCATGAGAACCACG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218839 representing NM_001033379
  Red=Cloning site Green=Tags(s)
  
  MAAAAAGGLPGKGHDISLAALRRHDPYISRIVDVASQVALYTFGHRANEWEKTGVEGTLFVYTRSASPKH
  GFTIMNRLSMENRTEPITKDLDFQLQNPFLLYRNGTLSIYGIWFYDKEECQRIAKLMKNLTQSEQLKACH
  GAGSSPVTLSSGEGQEVDILQMLTKAKDEYTKCKTCSEPKQMTNSSAICDNPKLIKPVPVRPSSSQRLQG
  PAPSKTSDPEPQHLSLTALFGKQDKAPCQETVKPSRTFAHHHHHHHQQQQETRPVHHGVACSLSCEEPRK
  LSLPVEKQLCPAIQKLMLGSPGLHPLPQHPEQWSCKSGSPSPAGGILPGPVQLGSPWNGRVAHCTQSTCR
  GHKLLEQLQGAPGAVHKYNPCAPTGPAVATQVAPGQSVAQSQLVYFSGPLQPPAPGHQALRKEQGALPAQ
  AVSLSGSQESSPTVLPTQELLRKLQVVHQEQQAAPRPALAARFPVSAQGSGTEKPLEAWVSKTASMEKQA
  PLLQSTCAPLRETDNGLMALGGQELPAASSSLLLPLQNWESSTVASRPLTRLQLQEALLNLIQNDDNFLS
  IIYEAYLFSVTQAAMRTT
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033379
• ORF Size: 1734 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033379.3, NP_001028551.2
• RefSeq Size: 3311 bp
• RefSeq ORF: 1737 bp
• Locus ID: 319618
• Cytogenetics: 6 F1